Axioscope light microscope

Manufactured by Zeiss

Sourced in Germany, United States

The Axioscope light microscope is a high-quality optical instrument designed for scientific and industrial applications. It provides a clear and detailed view of small-scale specimens, enabling users to observe and analyze their structure and characteristics. The Axioscope is equipped with advanced optics and illumination systems to deliver precise and consistent imaging performance.

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Close spelling variants of this product

Axioscope (349 citations) Axioscope microscope (153 citations) AxioScope A1 light microscope (33 citations) Axioscope 40 light microscope (2 citations)

16 protocols using axioscope light microscope

Quantitative Muscle Fiber Analysis

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The images were obtained by a Zeiss™ Axioscope light microscope. For the procedure of image acquisition, we use random labeling of the histological slides to photograph and quantify the images with low bias. Subsequently, the morphometry was processed with the ImageJ™ software applied to slides with ATPase in each pH. We quantified the cross-section area (CSA) of Type I, Type II, and Type IIx myofibers (n=100/fiber type/group) in 200X magnification images. Data were analyzed using Kruskal- Wallis with Dunn’s post-hoc test (p<0.05) by GraphPad Prism™ 8.0.1.
The stereology analysis of numerical density (%) was performed using the ImageJ™ software in 200X magnification images of Type I, II, and IIx muscle fibers (n=10 images/group); they were measured by counting the number of myofibers per photographed field, and their group means percentage was calculated.^{7 (link)}

Pimentel Neto J., Rocha L.C., dos Santos Jacob C., Klein Barbosa G, & Ciena A.P. (2021). Postsynaptic cleft density changes with combined exercise protocols in an experimental model of muscular hypertrophy. European Journal of Histochemistry : EJH, 65(Suppl 1), 3274.

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Quantifying Mast Cell Dynamics in Muscle

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Mast cell counts and degranulation was assessed based on previously published methods^{37 (link)}. Briefly, longitudinal sections (20um) of tibialis anterior muscle were made from fresh frozen tissue. Sections were washed and hydrated 2 times in distilled water for 10 minutes and embedded in 1% toluidine blue solution (Fisher Scientific, T16125) for 10–20 minutes. Slides were washed in distilled water and dehydrated in 70% ethanol, 95% ethanol, and in 100% ethanol. Slides were cleared in xylene and were mounted in ProLong mounting media (Invitrogen). Images were acquired using a Zeiss Axioscope light microscope. The number of toluidine blue-positive metachromatic mast cells were counted in six individual entire muscle section for each animal. The number of degranulating mast cells was also counted in each of these sections for each animal and were identified by extensive metachromatic granules being released by an isolated cell as described previously^{37 (link)}.

Granucci E.J., Griciuc A., Mueller K.A., Mills A.N., Le H., Dios A.M., McGinty D., Pereira J., Elmaleh D., Berry J.D., Paganoni S., Cudkowicz M.E., Tanzi R.E, & Sadri-Vakili G. (2019). Cromolyn sodium delays disease onset and is neuroprotective in the SOD1G93A Mouse Model of amyotrophic lateral sclerosis. Scientific Reports, 9, 17728.

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Quantitative Histological Analysis of Osteoarthritis

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Following micro-CT, the limbs were placed in 4% paraformaldehyde solution for 2 days and then decalcified for 12 days in 20% ethylene diamine tetra acetic acid(EDTA) in PBS (PH 7.0), processed, and embedded in paraffin wax, and 5μm coronal sections were obtained following the OARSI [44 (link)]. Serial sections were harvested every 75μm to encompass all weight-bearing areas of the knee joint. The sections were stained with 0.1% Safranin O/0.02% fast green to detect proteoglycans and glycosaminoglycans. Immunohistochemistry staining for Collagen II (Col2a1) (1:100, bs-10589R; Bioss, Beijing, China), Aggrecan (Acan) (1:200, bs-11655R; Bioss), MMP3(1:50, Ab52915; Abcam, Cambridge, UK), MMP13 (1:200, Ab39012; Abcam), IL6 (1:100, ab290735; Abcam) and TNFα(1:1000, ab307164; Abcam) were conducted on paraffin sections following appropriate antigen retrieval methods. The sections were imaged via an Axio Scope Light Microscope (Zeiss). The tibial plateau quadrants of the knee joint were scored by two independent blinded observers according the mouse recommendations of OARSI [44 (link)]. OA severity is expressed by the mean maximum score.

Wang H., Zhang C., Zhu S., Gao C., Gao Q., Huang R., Liu S., Wei X., Zhang H., Wei Q, & He C. (2023). Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints. PLOS ONE, 18(8), e0270074.

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Quantitative Assessment of Alveolar Epithelial Cells

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Lungs were analyzed using an Axioscope light microscope (Zeiss, Oberkochen, Germany) equipped with a computer-assisted stereology tool box (Cast 2.0; Olympus, Ballerup, Denmark).
Using a multipurpose test system stereological methods were performed on the light and electron microscopic level according to the guidelines for quantitative assessment of lung structure [40 (link)]. Alveolar epithelial cells type II (AEII) were collected according to the systematic random sampling [41 (link)] using a transmission electron microscope (TEM) (Morgagni II 268, Fa. FFEI, Oregon USA) provided with a digital camera (Veleta CCD, Olympus SIS, Münster, Germany). Stereological evaluation was performed using the software Stepanizer^®stereology tool Version 1 [42 (link)].
The following parameters were determined using the point (P) and intersection (IS) counting method [43 (link),44 (link)].

Schmiedl A., Roolfs T., Tutdibi E., Gortner L, & Monz D. (2017). Influence of prenatal hypoxia and postnatal hyperoxia on morphologic lung maturation in mice. PLoS ONE, 12(4), e0175804.

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Immunohistochemical Analysis of CUX1 and α-SMA

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For formalin-fixed paraffin-embedded specimens, tissues were fixed and hydrated as previously described [10] (link). For immunohistochemistry, sections were pre-treated with methanol (VWR, Lutterworth, UK), followed by antigen retrieval in heated 10 mM citrate buffer (pH 6). The primary antibodies were CUX1 antibody (0.2 mg/ml) and monoclonal anti-α-SMA clone 1a Cy3-conjugated antibody (0.7 mg/ml). Sections were sequentially incubated with a 1/200 dilution of an Alexa 488 secondary antibody. Slides were viewed and photographed using a Zeiss Axioscope light microscope with Axiovision software.

Ikeda T., Fragiadaki M., Shi-wen X., Ponticos M., Khan K., Denton C., Garcia P., Bou-Gharios G., Yamakawa A., Morimoto C, & Abraham D. (2016). Transforming growth factor-β-induced CUX1 isoforms are associated with fibrosis in systemic sclerosis lung fibroblasts. Biochemistry and Biophysics Reports, 7, 246-252.

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TEM Analysis of Noise-Induced Cochlear Damage

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Morphological damage related to noise exposure was investigated using TEM of the basal cochlear region. Animals were decapitated under deep anesthesia, and their cochleae were prepared according to a standard protocol for fixation and plastic embedding. Semi-thin sections were observed under a Zeiss Axioscope light microscope, and ultrathin radial sections of the organ of Corti were analyzed using TEM (n = 5–6 cochleae per time point).

Gratias P., Nasr J., Affortit C., Ceccato J.C., François F., Casas F., Pujol R., Pucheu S., Puel J.L, & Wang J. (2021). Impulse Noise Induced Hidden Hearing Loss, Hair Cell Ciliary Changes and Oxidative Stress in Mice. Antioxidants, 10(12), 1880.

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Generation and Characterization of A. citrulli M6 lip1 Mutant

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The M6-lip1^- mutant was screened out of a random transposon library that contains about 10,000 mutants, and was generated in the background of A. citrulli M6 using the Ez-Tn5 kit (Epicentre, Madison, WI, USA), as described [11 (link),16 (link)]. Mutants altered in twitching motility were directly screened on NA/Km selection plates by the naked eye, and verified by colony microscopy observations using an Axio Scope light microscope (Carl Zeiss, Jena, Germany) equipped with a DXM1200F digital camera (Nikon, Tokyo, Japan). The M6-lip1^- mutant was tested by Southern blot hybridization to verify insertion of the EZ-Tn5 cassette, using the ECL Direct Nucleic Acid Labeling and Detection System (Amersham Biosciences, Buckinghamshire, UK) according to the manufacturers’ instructions, and using a region of the EZ-Tn5 cassette as probe [11 (link)]. The insertion site of the EZ-Tn5 cassette was identified following sequencing of the mutant genome by Illumina MiSeq at the Center for Genomic Technologies of the Hebrew University of Jerusalem. Quality trimming and genome assembly were conducted as described [14 (link)].

Rosenberg T., Jiménez-Guerrero I., Tamir-Ariel D., Yarnitzky T, & Burdman S. (2022). The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium Acidovorax citrulli. Microorganisms, 10(5), 1016.

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Histological Analysis of Cell Samples

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For this purpose, the samples were stained with Hematoxylin/Eosin and analyzed under an AxioScope light microscope (Carl Zeiss, Jena, Germany)

Bustamante-Barrientos F.A., Méndez-Ruette M., Molina L., Koning T., Ehrenfeld P., González C.B., Wyneken U., Henzi R, & Bátiz L.F. (2023). Alpha-SNAP (M105I) mutation promotes neuronal differentiation of neural stem/progenitor cells through overactivation of AMPK. Frontiers in Cell and Developmental Biology, 11, 1061777.

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Crustacean Specimen Collection and Preservation

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The animals were sampled using a baited trap. The trap consists of a clear 500 ml screw-cap wide mouthed polythene jars with a dozen 8 mm holes on the bottle cap. Fresh fish used as bait were wrapped in cheese cloth. Animals caught were fixed in 4% formaldehyde sea water solution and later transferred into glycerol. Drawings were made using a camera lucida on a Zeiss Axioscope light microscope. The specimens were dissected and appendages and mouthparts mounted onto slides in glycerol.
Type materials were deposited in the South China Sea Research and Repository Centre, Institute of Oceanography and Environment, Universiti Malaysia Terengganu, 21030 Kuala Terengganu, Terengganu, Malaysia.

Othman B.H., Toda T, & Kikuchi T. (2016). A new species of Nebalia (Crustacea, Leptostraca) from coral reefs at Pulau Payar, Malaysia. ZooKeys.

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Immunohistochemical Analysis of CXCR4 and CD133

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Formalin-fixed and paraffin-embedded sections were subjected to high-temperature antigen retrieval and stained using Histostain-Plus Streptavidin-Peroxidase Detection kit (Life Technologies). Primary antibodies for IHC used were: Monoclonal Anti-Human CXCR4 (mab172, clone 44716, diluition 1:1000 R&D system), and Monoclonal Anti-human CD133/1 (clone AC133, diluition 1:100, Miltenyi Biotec, CA, USA,). Staining for CXCR4 and CD133 was categorized into semiquantitative classes based on the rate of stained (positive) tumour cells in 10 high power tumour field (400x)/slide: rated as negative moderate (<50% of cancer cells) and high expression (>50% of cancer cells) for CXCR4; rated as negative, focally low (<10% cancer cells) and focally high CD133 expression (>10% stained cancer cells). Semiquantitative classes were chosen by our pathologists after consensus discussion and careful revision of all slides. The distribution of the CXCR4 and CD133 protein was analyzed by live imaging using a Zeiss AxioScope light microscope.

Cioffi M., D’Alterio C., Camerlingo R., Tirino V., Consales C., Riccio A., Ieranò C., Cecere S.C., Losito N.S., Greggi S., Pignata S., Pirozzi G, & Scala S. (2015). Identification of a distinct population of CD133+CXCR4+ cancer stem cells in ovarian cancer. Scientific Reports, 5, 10357.

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