Millipore water purification system

Manufactured by Merck Group

Sourced in United States, Germany, Ireland, Italy, France

The Millipore water purification system is a laboratory equipment designed to produce high-quality, ultrapure water. It utilizes a series of filtration and purification steps to remove impurities, ensuring the water meets specific purity standards for use in various applications within the research and scientific community.

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Lab products found in correlation

Methanol, by Merck Group (13 mentions) Formic acid, by Merck Group (12 mentions) Acetonitrile, by Merck Group (9 mentions) Methanol, by Thermo Fisher Scientific (6 mentions) Potassium chloride (kcl), by Merck Group (4 mentions) Acetonitrile of hplc grade, by Merck Group (4 mentions) Milli q, by Merck Group (4 mentions) Gallic acid, by Merck Group (4 mentions) Acetonitrile, by Thermo Fisher Scientific (4 mentions) Acetic acid, by Merck Group (3 mentions) Sucrose, by Merck Group (3 mentions) Potassium hydroxide, by Merck Group (3 mentions) Hplc grade acetonitrile, by Merck Group (3 mentions) Hydrochloric acid (hcl), by Merck Group (3 mentions) Hepes, by Merck Group (3 mentions) Acetone, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions) Chloroform, by Merck Group (2 mentions) Phosphoric acid, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)

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77 protocols using millipore water purification system

Quantification of Pesticides and Pharmaceuticals

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Ametryn and
atrazine were acquired
from Riedel de Haën (Seelze, Germany). Isoprocarb was purchase
from Chem Service (West Chester, PA). Methylamine was purchased from
Alfa Aesar (Ward Hill, MA). Acetonitrile, acetone, methanol, and toluene
were obtained from Merck (Darmstadt, Germany), Macron Fine Chemicals
(Center Valley, PA), Echo Chemical, and Avantor Performance Materials
(Corporate Parkway, PA), respectively. Ultrapure water (18.2 Ω)
was obtained from a Merck Millipore water purification system (Billerica,
MA). Mint, ginger, garlic, catmint, banana, tomato, coffee beans,
mothballs, plumeria, Inderal, and Panadol tablets were purchased from
local shops.

Huang D.Y., Tsai J.J, & Chen Y.C. (2022). Direct Mass Spectrometric Analysis of Semivolatiles Derived from Real Samples at Atmospheric Pressure. ACS Omega, 7(12), 10255-10261.

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Lipid Oxidation Characterization Protocol

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Lipoid S75, Lipoid S90 and Lipoid S100 were purchased from Lipoid GmbH, Ludwigshafen, Germany.
Methanol (99%) was supplied by Carl Roth GmbH, Karlsruhe, Germany.
Potassium chloride, hydrogen peroxide, phosphoric acid were supplied by Merck, Darmstadt, Germany.
Ethanol (96%) was purchase from Vilnius degtine, Vilnius, Lithuania.
TBA (thiobarbituric acid), tris-HCl (tris-hydrochloride), DTNB (5,5-dithiobis-(2-nitrobenzoic acid) were supplied by Serva, Heidelberg, Germany.
Formic acid was purchased from Fluka Chemie, Buchs, Switzerland.
Purified water was produced using a Millipore water purification system (Merck, Rahway, NJ, USA).
Acetonitrile, chloroform (99.8%), acetic acid (99.8%), thymol (99%) and CA (98%) were supplied by Sigma-Aldrich, Buchs, Switzerland.
Helium (99.999%) was supplied by AGA, Vilnius, Lithuania.

Kubiliene A., Munius E., Songailaite G., Kokyte I., Baranauskaite J., Liekis A, & Sadauskiene I. (2023). A Comparative Evaluation of Antioxidant Activity of Extract and Essential Oil of Origanum onites L. In Vivo. Molecules, 28(14), 5302.

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Analytical Methods for Cannabinoid Determination

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Potassium chloride, phosphoric acid, and hydrogen peroxide were purchased from Merck (Darmstadt, Germany). Ethanol (96%) was supplied from Vilnius Degtine (Vilnius, Lithuania). Purified water was produced using a Millipore water purification system (Merck, Kenilworth, NJ, USA). Ammonium molybdate, ammonium formate ABTS (2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate), and DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) reagents were purchased from Sigma-Aldrich (Schnelldorf, Germany). Acetonitrile was purchased from Sigma-Aldrich (Buchs, Switzerland). Formic acid was purchased from Fluka Chemie (Buchs, Switzerland). DTNB (5,5-dithiobis-(2-nitrobenzoic acid), tris-HCl (tris-hydrochloride), and TBA (thiobarbituric acid) were purchased from Serva (Heidelberg, Germany). Standard solutions of cannabinoids (CBD, CBDA, CBG) and AlCl₃ (>99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Kubiliene A., Mickute K., Baranauskaite J., Marksa M., Liekis A, & Sadauskiene I. (2021). The Effects of Cannabis sativa L. Extract on Oxidative Stress Markers In Vivo. Life, 11(7), 647.

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Enzyme-Based Electrochemical Biosensor Fabrication

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Poly(vinyl alcohol) (PVA, M_w ~195000), maleic acid (MA) and ascorbic acid were purchased from Aladdin Reagent Company (Shanghai, China). Chloroplatinic acid hexahydrate (H₂PtCl₆∙6H₂O) and palladium chloride (PdCl₂) were bought from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). Alcohol oxidase enzyme (AOX) extracted from Pichia pastoris (10-40 units/mg protein), glucose oxidase (GOX), 2-(N-morpholino)-ethanesulphonic acid (MES, ≥99%), potassium acetate (KAc) and Pluronic F-127 were supplied by Sigma Chemical Co. (St. Louis, MO, USA). Hydrogen peroxide (H₂O₂, 30%), hydrochloric acid and sulfuric acid were obtained from Beijing Chemical Works (Beijing, China). 3,3,5,5-tetramethylbenzidine (TMB, >99.0%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). The double deionized water (hereinafter denoted as H₂O for short) was used in all experiments (Millipore water purification system, Merck, Darmstadt, Germany). All the chemicals and reagents were used without further purification.

Zhang Y., Liu Q., Ma C.B., Wang Q., Yang M, & Du Y. (2020). Point-of-care assay for drunken driving with Pd@Pt core-shell nanoparticles-decorated ploy(vinyl alcohol) aerogel assisted by portable pressure meter. Theranostics, 10(11), 5064-5073.

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Nanoparticle Synthesis for Bioactive Compound Extraction

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Ginsenoside Rg₁ (Rg₁), ginsenoside Rb₁ (Rb₁), notoginsenoside R₁ (R₁), ursolic acid (UA), oleanolic acid (OA) were supplied by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Poly (ethylene glycol)-block-poly (propylene glycol)-block-poly (ethyiene glycol) (P123), tetraethoxy silane (TEOS), tetrahydrofuran, ethylene glycol dimethacrylate (EGDMA) and 4-vinylpyridine (4-VP) were purchased from Sigma-Aldrich (Steinheim, Germany). Acrylamide (AM), methacrylic acid (MAA) and 2,2-azobisisobutyronitrile (AIBN) from Sinopharm (Shanghai, China). 3-Methacryloxypropyltrimethoxysilane (MPS) was obtained from Aladdin (Shanghai, China). Acetonitrile of HPLC grade was prepared from Merck (Darmstadt, Germany). Ultrapure water (18.2 MΩ cm) was produced using a Millipore water purification system (Merck Millipore, Darmstadt, Germany) and used for the entire experiment. All other chemicals used in this study were of the analytical reagent grade.
The powder of Panax notoginseng was donated by the Department of Pharmacognosy, Nanjing Medical University (Nanjing, China).

Sun C., Wang J., Huang J., Yao D., Wang C.Z., Zhang L., Hou S., Chen L, & Yuan C.S. (2017). The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples. Polymers, 9(12), 653.

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Microchip Fabrication Protocol

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All chemicals were of molecular biology grade and purchased from Sigma-Aldrich. Ultrapure water used in these processes came from a Millipore water purification system (Merck Millipore, USA). Oligonucleotides were provided by STABVIDA, Portugal. SU-8 2050 photoresist, propylene glycolmethyl ether acetate developer (PGMEA) and customized masks, used during the lithography process of the microchip fabrication, were obtained from Microchem (Microchem, USA) and JDPhoto (JDPhoto-tools, UK), respectively. Epoxy resin ES562, used as a master mold for PDMS soft lithography, was ordered from Permabond (Permabond, UK). For the PDMS microchips fabrication, a Sylgard 184 Silicone Elastomer Kit was used (DowCorning, Spain). To improve separation between the PDMS and SU-8 mold, a silanization step was performed onto the SU-8 mold using tridecafluoro-1,1,2,2-tetrahydrooctyl trichlorosilane (Microchem, USA). Microfluidic connections between chip and reservoirs were made using an optimized Teflon tubing kit, ordered from Elveflow (ElveSys SAS, France).

Alves P.U., Vinhas R., Fernandes A.R., Birol S.Z., Trabzon L., Bernacka-Wojcik I., Igreja R., Lopes P., Baptista P.V., Águas H., Fortunato E, & Martins R. (2018). Multifunctional microfluidic chip for optical nanoprobe based RNA detection – application to Chronic Myeloid Leukemia. Scientific Reports, 8, 381.

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Antibody-Targeted Liposomal Drug Delivery

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Soybean phosphatidylcholine (SPC) was purchased from Taiwei Pharmaceutical Co, Ltd (Shanghai, People’s Republic of China). N-[(3-maleimide-1-oxopropyl)aminopropyl polyethyleneglycol-carbamyl] distearoylphosphatidyl-ethanolamine (DSPE-PEG-MAL) was purchased from NOF America Corporation (White Plains, NY, USA). DTX was supplied by LC Laboratories (Woburn, MA, USA). The rabbit monoclonal anti-CA IX antibody targeting the N-terminal region of the antigen protein that is exposed to the extracellular side was obtained from Abcam (Cambridge, UK). The goat antirabbit horseradish peroxidase (HRP)-conjugated secondary antibody was obtained from Bio-rad Laboratories (Hercules, CA, USA). Dithiotreitol (DTT) was purchased from Sigma-Aldrich (St Louis, MO, USA). The lipophilic near-infrared fluorescent dye 1,1′-dioctadecyltetramethyl indotricarbocyanine iodide (DiR) used for labeling liposomes was supplied by Caliper LifeSciences (Hopkinton, MA, USA). Acetonitrile of HPLC grade and ethyl acetate were obtained from Anaqua Chemicals Supply (Houston, TX, USA). Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich. Ultrapure water was generated by a Millipore water purification system (EMD Millipore, Billerica, MA, USA).

Wong B.C., Zhang H., Qin L., Chen H., Fang C., Lu A, & Yang Z. (2014). Carbonic anhydrase IX-directed immunoliposomes for targeted drug delivery to human lung cancer cells in vitro. Drug Design, Development and Therapy, 8, 993-1001.

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Cantharidin-loaded Lipid Nanocarriers

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Cantharidin was purchased from Chengdu Biopurify Phytochemicals Ltd (Sichuan, China); Soy phosphatidylcholine (SPC) was purchased from Taiwei Pharaceutical Co. Ltd (Shanghai, China); 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG₂₀₀₀) was purchased from Avanti Polar Lipids Inc. (Alabaster, AL, USA); 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) was purchased from Invitrogen. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich. Near-infrared (NIR) lipophilic carbocyanine dye 1,1′-dioctadecyltetramethyl indotricarbocyanine iodide (XenoLight DiR) was purchased from Caliper Life Sciences (Hopkinton, MA, USA). Ultrapure water was generated by a Millipore water purification system (EMD Millipore, Billerica, MA, USA). All reagents and chemicals were of analytical grade.

Zhang X., Lin C.C., Chan W.K., Liu K.L., Yang Z.J, & Zhang H.Q. (2017). Augmented Anticancer Effects of Cantharidin with Liposomal Encapsulation: In Vitro and In Vivo Evaluation. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(7), 1052.

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Quantification of Sulforaphane Metabolites

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LC–MS-grade acetonitrile was from Burdick and Jackson (Muskegon, MI, USA). LC–MS-grade formic acid (Optima^TM) was from Fisher Chemical (Thermo Fisher Scientific, Waltham, MA, USA). Reverse osmosis purified MilliQ water used in LC–MS analysis was from Millipore water purification system (Merck, Darmstadt, Germany). Analytical standards SFN, SFN-d₈, SFN-NAC-d₈, SFN-GSH, SFN-NAC, SFN-Cys were from Toronto Research Chemicals (Toronto Research Chemicals, Toronto, ON, Canada). CysGly (>85%) and pyridine were purchased from Sigma Aldrich (St. Louis, MI, USA). HF Bond Elute^TM SPE cartridges C18 (6 mL tube, 500 mg bed) were purchased from Agilent Technologies (Colorado Springs, CO, USA). Myrosinase-activated broccoli sprout extract capsules, Broccomax^TM, were sourced from Jarrow Formulas (Los Angeles, CA, USA).

Langston-Cox A., Anderson D., Creek D.J., Palmer K., Wallace E.M, & Marshall S.A. (2020). Measuring Sulforaphane and Its Metabolites in Human Plasma: A High Throughput Method. Molecules, 25(4), 829.

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Chemical Standards for UHPLC-Q-TOF-MS Analysis

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Deionized water was prepared by using a Millipore water purification system (Merck, Hong Kong, China). LC-MS grade acetonitrile, methanol, and formic acid were bought from Anaqua Global International Inc., Limited (United States). LPS (Escherichia coli 0127: B8) and dexamethasone were obtained from Sigma Chemical Co. (St. Louis, MO, United States). Chemical standards for UHPLC-Q-TOF-MS analyses were purchased from Chengdu Must Bio-Technology Co., Ltd. (Apigenin ≥ 98%, Baicalein ≥ 98%, Chrysin ≥ 98%, scutellarein ≥ 98%, Wogonin ≥ 98%, Oroxylin A ≥ 98%, and Baicalin ≥ 98% in purity), and Innochem (Isoscutellarein ≥ 95%, and Wogonoside ≥ 98% in purity), respectively.

Liu J., Meng J., Li R., Jiang H., Fu L., Xu T., Zhu G.Y., Zhang W., Gao J., Jiang Z.H., Yang Z.F, & Bai L.P. (2022). Integrated network pharmacology analysis, molecular docking, LC-MS analysis and bioassays revealed the potential active ingredients and underlying mechanism of Scutellariae radix for COVID-19. Frontiers in Plant Science, 13, 988655.

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