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Z series coulter counter

Manufactured by Beckman Coulter
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The Z Series Coulter Counter is a laboratory instrument used for counting and sizing particles, such as cells, in a liquid sample. It utilizes the Coulter principle to detect and measure the changes in electrical impedance as particles pass through a small aperture, providing accurate and precise particle count and size data.

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Lab products found in correlation

70μm filtration, by BD (4 mentions) Ficoll paque, by GE Healthcare (4 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (4 mentions) Giemsa, by Merck Group (2 mentions) Rbc lysis buffer, by Thermo Fisher Scientific (2 mentions) Transwell permeable support, by Corning (1 mentions) 6 well culture plate, by Greiner (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) 2470 wizard2, by PerkinElmer (1 mentions) Hamamatsu slide scanner, by Hamamatsu Photonics (1 mentions) Elisa, by R&D Systems (1 mentions) Minicollect, by Greiner (1 mentions) Lymphocyte separation medium, by Corning (1 mentions) Rosettesep human nk cell enrichment cocktail, by STEMCELL (1 mentions) Crystal violet, by Merck Group (1 mentions) Epoch microplate spectrophotometer, by Agilent Technologies (1 mentions) Coulter isoton 2 diluent, by Beckman Coulter (1 mentions) Hsc 3, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Ca9 22, by Japanese Collection of Research Bioresources Cell Bank (1 mentions) Hsc 2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)

28 protocols using z series coulter counter

1

Monitoring Trypanosome Cell Growth

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Wild-type 29-13 and TbSPHK RNAi cells were seeded at an initial density of 106 cells/ml, and RNAi induction was initiated by the addition of 1 µg/ml tetracycline. Cell density was monitored daily using a Z-series Coulter Counter with a lower threshold set to 3 µm and an upper threshold set to a 10-µm diameter. Cultures were diluted to 106 cells/ml with fresh medium every 2 days to maintain parasites in the logarithmic phase of growth.
Pasternack D.A., Sharma A.I., Olson C.L., Epting C.L, & Engman D.M. (2015). Sphingosine Kinase Regulates Microtubule Dynamics and Organelle Positioning Necessary for Proper G1/S Cell Cycle Transition in Trypanosoma brucei. mBio, 6(5), e01291-15.
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2

Co-culture Cell Proliferation Assay

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WI-38 cells were growth to 70–80% confluency on transwell permeable supports (Costar, Cat# 3412), treated with control siRNA or siRNA against G3BP1 and irradiated. Eight days following irradiation, 50,000 A549 or CBSF-4T cell lines were plated on 6-well dishes and supports containing no cells, or either control siRNA or siRNA against G3BP1 treated cells were added above the cells. Total cell number was counted every 24 h using a Beckman Z-series Coulter Counter after trypsinization.
Omer A., Barrera M.C., Moran J.L., Lian X.J., Di Marco S., Beausejour C, & Gallouzi I.E. (2020). G3BP1 controls the senescence-associated secretome and its impact on cancer progression. Nature Communications, 11, 4979.
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3

Nutritional Stress Induces Trypanosoma Epimastigote Proliferation

Cited 2 times
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Nutritional stress was performed as previously described (36 (link)). Briefly, epimastigotes at the end of the exponential growth phase (5-day cultures at a density of ≥5 × 107 parasites·mL-1) were harvested by centrifugation at 5,000 × g for 5 min at room temperature, washed twice with phosphate buffered saline (PBS, pH 7.4), and incubated for 2 h at 28°C in TAU medium (190 mM NaCl, 17 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 8 mM phosphate buffer pH 6.0) at a density of 5 × 108 parasites·mL-1. For cell proliferation assay, cultures starting with 1 × 106 epimastigotes·mL-1 were monitored throughout 7 days, and parasite density was determined every 24–48 h. We used the automatic particle counter Z Series Coulter Counter (Beckman) to perform the cellular count. All experiments were performed at least in technical and biological triplicates, and one-way analysis of variance statistically analyzed the data. Graphs and statistics were obtained using the GraphPad Prism software version 8.4.2 (La Jolla, CA, USA). The immunofluorescence assay was performed as previously described (23 (link)).
Romagnoli B.A., Lucena A.C., Freire E.R., Munhoz da Rocha I.F., Alves L.R, & Goldenberg S. (2024). TcZC3HTTP, a regulatory element that contributes to Trypanosoma cruzi cell proliferation. Microbiology Spectrum, 12(3), e02880-23.
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4

Quantifying Arsenic-Induced Cell Death

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Cells were plated at 30,000 cells per well in a 24-well plate for 24 hours, after which cells were treated with increasing concentrations of iAs(III) or MMA(III) for 24 hours. Cells were then trypsinized with 0.25% trypsin to obtain aliquots of cell suspension. Active measurements of cell death induced by arsenic toxicity were conducted by manually counting aliquots of cell suspension by using a 1:1 dilution of Trypan Blue Exclusion dye. The lethal concentrations at 50% (LC50) for MMA(III) and iAs(III) at 24 hours were determined using Origin software (version 6.0). To complement our Trypan Blue Exclusion assays, cell viability was determined by performing cell counts of cell suspensions of A7r5 cells treated with increasing concentrations of arsenicals by using a Z™ series-Coulter Counter (Beckman Coulter) (Figure S3).
Pace C., Banerjee T.D., Welch B., Khalili R., Dagda R.K, & Angermann J. (2016). Monomethylarsonous Acid, But Not Inorganic Arsenic, is a Mitochondria-Specific Toxicant in Vascular Smooth Muscle Cells. Toxicology in vitro : an international journal published in association with BIBRA, 35, 188-201.
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5

Evaluating Compound Effects on Anaerobic Growth

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Cultures to assess impact on growth rates and survival were seeded at 2×105 cells/mL in 6-well culture plates (Greiner Bio-One, Germany) for several of the most active compounds identified in the resorufin drug-sensitivity assay, at 2×EC50 concentration, and incubated under microaerobic conditions at 37 ºC. At 8-h intervals samples were taken from each well for cell counts, using a Beckman Z Series Coulter Counter, and for fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI (Vector Laboratories, Burlingame)). Due to the rapid growth of the control (no drug) culture, cultures were passaged after 24 h, back to 1.32×105 cells/mL, in fresh medium. Metronidazole was used as a positive control and untreated cells were used as a negative control.
Natto M.J., Hulpia F., Kalkman E.R., Baillie S., Alhejeli A., Miyamoto Y., Eckmann L., Van Calenbergh S, & de Koning H.P. (2021). Deazapurine nucleoside analogues for the treatment of Trichomonas vaginalis. ACS infectious diseases, 7(6), 1752-1764.
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6

Growth Curve and Metacyclogenesis Analysis

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For growth curve analysis, epimastigote cultures were established at a density of 1 x 106 cells ml-1 and population growth was monitored for seven days, with cell counting in a Z series Coulter Counter (Beckman Coulter, CA, USA). Experiments were performed in technical and biological triplicates and the data were analyzed with the t-test using GraphPad software.
To quantify metacyclogenesis, epimastigotes were allowed to differentiate in vitro as described above (see “Parasite”), and the number of metacyclic trypomastigotes was counted in a Neubauer chamber after 72h of incubation in TAU3AAG medium (which corresponds to the peak of differentiation into metacyclic trypomastigotes). Experiments were performed in technical triplicates.
Moreira C.M., Batista C.M., Fernandes J.C., Kessler R.L., Soares M.J, & Fragoso S.P. (2017). Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain. PLoS ONE, 12(7), e0179615.
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7

Quantification of Peritoneal Neutrophils

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Peritoneal lavage was collected by injection and aspiration of 2 ml PBS. The total number of living cells in the peritoneal lavage, lysed with lyserglobin, was counted using a Z™ Series coulter counter® (Beckman Coulter, Brea, CA, USA). The lavage was diluted to 500,000 cells/ml, and 100 µl of cell solution was applied for cytospin preparation. The cytospin slides were stained with Giemsa (Merck Millipore, Billerica, MA, USA) for 1 h at room temperature. The stained slides were scanned using a Hamamatsu slide scanner (Hamamatsu Photonics, Hamamatsu, Japan), and neutrophils were counted using morphological features in 250 cells (37 (link)). The total number of neutrophils was calculated using the total cell count of the peritoneum and the neutrophil counts from cytospin preparations.
Sahasrabudhe N.M., Beukema M., Tian L., Troost B., Scholte J., Bruininx E., Bruggeman G., van den Berg M., Scheurink A., Schols H.A., Faas M.M, & de Vos P. (2018). Dietary Fiber Pectin Directly Blocks Toll-Like Receptor 2–1 and Prevents Doxorubicin-Induced Ileitis. Frontiers in Immunology, 9, 383.
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8

Viability Assay with PICALM siRNA

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Viability studies were performed after incubation with siRNA to total PICALM for 48 and 72 h using the CellTiter 96® MTS Aqueous One Solution Cell Proliferation Assay (Promega) following the manufacturer’s guidelines. The total number of cells was also counted using a Z™ Series Coulter Counter (Beckman Coulter (UK) Ltd, High Wycombe, UK).
Thomas R.S., Henson A., Gerrish A., Jones L., Williams J, & Kidd E.J. (2016). Decreasing the expression of PICALM reduces endocytosis and the activity of β-secretase: implications for Alzheimer’s disease. BMC Neuroscience, 17, 50.
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9

Mouse Splenocyte Isolation and Enumeration

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PBS (Invitrogen, Carlsbad, CA, USA) and separated by Ficoll-Paque (GE Healthcare, Piscataway, NJ, USA) centrifugation. Mouse splenocytes were isolated by disaggregation and 70μm filtration (BD, San Diego, CA, USA) to form a single cell suspension. Cells were enumerated using a Z™ Series Coulter Counter (Beckman Coulter Inc., NSW, Australia).
Saulep-Easton D., Vincent F.B., Quah P.S., Wei A., Ting S.B., Croce C.M., Tam C, & Mackay F. (2015). The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 30(1), 163-172.
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10

Optimized Mass Spectrometry Lipidomics and Proteomics

Cited 1 time
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Reagents and solvents were purchased from Fisher Scientific and used without further purification. The iodoTMTsixplex isobaric label reagent set was purchased from Fisher Scientific and used according to the manufacturer’s instructions with some modifications described in Section 2.4.3. Mass spectrometry of lipid samples was performed by Kansas State University Lipidomics Research Center using a 4000 QTRAP LC-MS/MS in positive ion mode. Mass spectrometry of TMT-labeled peptides was performed by the University of Texas Southwestern Proteomics Core using an Orbitrap Fusion Lumos mass spectrometer coupled to an Ultimate 3000 RSLC-Nano liquid chromatography system in positive ion mode. KbHT and KbLT cultures were grown under continuous illumination under identical conditions as previously described (Chen, Colon et al. 2018 (link)). Growth was monitored by counting a 1:10 dilution of culture in Z pak reagent using a Beckman Z-series Coulter Counter with aperture size between 10 30mm, according to the manufacturer’s instructions.
Ma Z.Q., Spreafico E., Pollio G., Santagati S., Conti E., Cattaneo E, & Maggi A. (1993). Activated estrogen receptor mediates growth arrest and differentiation of a neuroblastoma cell line.. Proceedings of the National Academy of Sciences of the United States of America, 90(8), 3740-3744.
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