Potassium hexacyanoferrate 2 trihydrate

Manufactured by Merck Group

Sourced in United States, Germany, Italy

Potassium hexacyanoferrate (II) trihydrate is a chemical compound that is commonly used in various laboratory applications. It is a crystalline solid with the chemical formula K4[Fe(CN)6]·3H2O. The compound is a source of the ferrocyanide ion, which can be used in a variety of analytical and synthetic procedures.

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Lab products found in correlation

Potassium hexacyanoferrate 3, by Merck Group (35 mentions) Potassium chloride (kcl), by Merck Group (13 mentions) Formic acid, by ITW Reagents (11 mentions) Zinc acetate dehydrate, by Merck Group (11 mentions) 13c3 labelled acrylamide, by Cambridge Isotopes (9 mentions) Methanol, by ITW Reagents (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Iron 3 chloride hexahydrate, by Merck Group (8 mentions) Sodium chloride (nacl), by Merck Group (8 mentions) Milli q integral 5 water purification system, by Merck Group (7 mentions) Sodium hydroxide (naoh), by Merck Group (6 mentions) Ethanol, by Merck Group (6 mentions) Bovine serum albumin (bsa), by Merck Group (6 mentions) N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride, by Merck Group (5 mentions) Citric acid, by Merck Group (5 mentions) D glucose, by ITW Reagents (5 mentions) Hydrochloric acid (hcl), by Merck Group (5 mentions) Dimethyl sulfoxide (dmso), by Merck Group (5 mentions) Sulfuric acid, by Merck Group (4 mentions) N hydroxysuccinimide nhs, by Merck Group (4 mentions)

87 protocols using potassium hexacyanoferrate 2 trihydrate

Platelet Factor 4 Binding Assay

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The following reagents were
used: cysteamine-hydrochloride (Fluka, Sigma-Aldrich, 30080, Germany),
ethanolamine (Sigma-Aldrich, E9508, Germany), glycine (Sigma-Aldrich,
092K0099, Germany), glutaraldehyde (Sigma-Aldrich grade I 25%), potassium-hexacyanoferrate
(II) trihydrate (Merck, A870884, Darmstadt, Germany), potassium-hexacyanoferrate(III)
trihydrate (Merck, A81167372124, Darmstadt, Germany), RTO and KKO
(Invitrogen, Thermo Fisher, Germany), platelet factor 4 (Chromatec,
Greifswald, Germany), UFH Heparin-Natrium-25000 (Ratiopharm, Ulm,
Germany), and phosphate buffer saline (PBS) pH7.4. All of the chemicals
were of the highest purity available and were used as purchased.

Khan N.Z., Chen L.Y., Lindenbauer A., Pliquett U., Rothe H, & Nguyen T.H. (2021). Label-Free Detection and Characterization of Heparin-Induced Thrombocytopenia (HIT)-like Antibodies. ACS Omega, 6(40), 25926-25939.

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Quantitative Determination of Polyphenols

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Silver nitrate (AgNO₃), anhydrous 2,9-dimethyl-1,10-phenanthroline (neocuproine: Nc),
sucrose, d-(+)-glucose, d-(−)-fructose, d-(+)-galactose, d-(+)-mannose, d-(+)-maltose
monohydrate, d-(+)-lactose monohydrate, (+)-catechin hydrate,
(−)-epicatechin, chlorogenic acid, gallic acid monohydrate, l-(+)-ascorbic acid, and potassium sodium tartrate tetrahydrate,
Discovery DPA-6S (250 mg, 3 mL), and Discovery DSC-18 (1 g, 6 mL)
SPE cartridges were purchased from Sigma-Aldrich (Steinheim, Germany).
Copper(II) chloride dihydrate, ammonia solution (NH₃),
sodium carbonate and sodium hydroxide (NaOH), potassium hexacyanoferrate(II)
trihydrate, and zinc sulfate heptahydrate were obtained from Merck
(Darmstadt, Germany). Sulfuric acid (H₂SO₄)
was obtained from Carlo Erba (Italy).

Durmazel S., Üzer A., Erbil B., Sayın B, & Apak R. (2019). Silver Nanoparticle Formation-Based Colorimetric Determination of Reducing Sugars in Food Extracts via Tollens’ Reagent. ACS Omega, 4(4), 7596-7604.

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Synthesis of Photocatalytic Thin Films

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Potassium hexacyanoferrate (III), potassium chloride, ethyl alcohol (95%), metallic iodine, and titanium dioxide (P25) were purchased from Sigma-Aldrich (San Luis, MO, USA). Potassium hexacyanoferrate (II) trihydrate was obtained from Merck (Darmstadt, HD, Germany). Acetone, acetylacetone (99.5%), ethyl alcohol, and isopropyl alcohol were obtained from Synth (São Paulo, SP, Brazil). ITO/glass substrates (15 Ω/sq) were acquired from Lumtec (Taiwan, China).

do Amaral Amâncio M., Romaguera-Barcelay Y., Matos R.S., Pires M.A., Gandarilla A.M., do Nascimento M.V., Nobre F.X., Ţălu Ş., da Fonseca Filho H.D, & Brito W.R. (2022). Effect of the Deposition Time on the Structural, 3D Vertical Growth, and Electrical Conductivity Properties of Electrodeposited Anatase–Rutile Nanostructured Thin Films. Micromachines, 13(8), 1361.

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Carbohydrate FODMAP Conversion Analysis

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To determine FODMAP conversion in the carbohydrate model system, 4 measuring intervals were defined based on the results of the previous growth experiments—6 h, 24 h, 48 h, and 72 h of incubation. The respective cell mass was transferred from the Honeycomb format plates into 2 mL centrifugation tubes. Proteins were removed by Carrez-precipitation. Hence, 15 µL of Carrez I solution—potassium hexacyanoferrate(II)trihydrate (Merck, Darmstadt, Germany) in 1000 mL H₂O, and 15 µL of Carrez II Solution—300 g zinc acetate dehydrate (VWR International GmbH, Darmstadt, Germany) in 1000 mL H₂O was added to precipitate the proteins. After centrifugation at 16,000× g for 30 min at 4 °C, the supernatant was transferred to a volumetric flask and filled up to 1 mL. Next, the solution was filtered through a 0.2-µm filter (Rotilabo Mini-Tip syringe filter; Carl Roth GmbH + Co. KG, Karlsruhe, Germany) and diluted to receive carbohydrate concentrations between 5–200 ppm and transferred into vials (1.5 mL clear glass; VWR International GmbH, Darmstadt, Germany) for further analysis.

Fraberger V., Call L.M., Domig K.J, & D’Amico S. (2018). Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs. Nutrients, 10(9), 1247.

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Immunization and Antibody Purification Protocol for Plasmodium falciparum HRP2

Cited 1 time

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DHP, potassium hexacyanoferrate(iii), and potassium chloride were purchased from Sigma-Aldrich (St. Louis, USA). potassium hexacyanoferrate(ii) trihydrate, sulfuric acid (98%), and buffer saline solution (PBS) of pH 7.00 were from Merck (Darmstadt, Germany). Purified polyclonal Ab-PfHRP2, Ag-PfHRP2, and human serum samples were obtained from Leônidas and Maria Deane Institute (Oswaldo Cruz Foundation, Manaus, Brazil). The biomolecules preparation process consisted of the recombinant expression of Plasmodium falciparum HRP2 in Escherichia coli BL21 pLysS (Invitrogen), and the purification was performed using affinity chromatography nickel columns (QIAGEN), following the manufacturer instructions.^{28 (link)} Later, mice were immunized with the purified recombinant protein (4 bi-weekly 10 μg doses). Serum samples were collected, and reactivity was analyzed by indirect enzyme-linked immunosorbent assay (ELISA). Antibodies were purified using the protein G sepharose (Sigma) resin column following the manufacturer's recommendations.
All the other reagents employed were of analytical grade and were used without further purification. Aqueous solutions were prepared by using purified water from a Milli-Q system.

Dip Gandarilla A.M., Regiart M., Bertotti M., Correa Glória J., Morais Mariuba L.A, & Ricardo Brito W. (2020). One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination. RSC Advances, 11(1), 408-415.

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HPLC Quantification of Ascorbic Acid

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All organic solvents, methanol and acetonitrile, were from J.T. Baker (Deventer, Holland) HPLC-grade. Water was purified by a Milli-Q-RO4 water purification system (Millipore, Bedford, MA, USA) with a resistivity of 10 MΩ·cm. The standard AA was purchased from Fluka (Deisenhofen, Germany). Potassium hexacyanoferrate (II) trihydrate and zinc sulfate heptahydrate were obtained from Merck (Darmstad, Germany). Oasis HLB 200 mg/6 mL SPE cartridges were obtained from Waters (Milford, MA, USA). Bond Elut-Accucat (200 mg/3 mL) SPE cartridges were purchased from Varian (Chicago, IL, USA) and used with a SPE vacuum manifold (Visiprep, Supelco, Bellefonte, PA, USA). Amber glass auto-sampler vials with septum screw caps were obtained from Agilent Technologies (Wilmington, DE, USA). The analytical column (Atlantis dC18, 250 × 4.6 mm/5) with pre-column and syringe filters (0.45 μm PVDF) were from Waters (Milford, MA, USA).
A standard AA stock solution (1.0 mg/mL) was prepared by dissolving 1.0 mg of AA in 1.0 mL of Milli-Q water. To weigh AA, an electronic analytical balance (Radwag, PCE group, 0.01 mg Radom, Poland) was used. The AA stock solution was diluted in amber glass volumetric flasks to prepare calibration standards at 50, 100, 200, 500, 800 and 1000 ng/mL respectively and stored at 4°C until use.

CAPEI R., PETTINI L., LO NOSTRO A, & PESAVENTO G. (2015). Occurrence of Acrylamide in breakfast cereals and biscuits available in Italy. Journal of Preventive Medicine and Hygiene, 56(4), E190-E195.

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Protocols for Protein Binding Experiments

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Scopoletin (7-Hydroxy-6-methoxycoumarin), sodium dodecyl sulfate (SDS), hydrogen peroxide and (11-mercaptoundecyl)tetra-(ethylene glycol) were purchased from Sigma Aldrich (Steinheim, Germany). Absolute ethanol (≥ 99.8 %), hydrochloric acid, potassium chloride and sodium hydroxide from VWR (Leuven, Belgium); dipotassium phosphate, potassium dihydrogen phosphate, potassium hexacyanoferrate (II) trihydrate and ammonia solution 25 % from Merck (Darmstadt, Germany); sulfuric acid (95 -98 %), nitric acid (≥ 65 %), sodium chloride, potassium hexacyanoferrate (III) and potassium hydroxide from Roth (Karlsruhe, Germany); disodium hydrogen phosphate dihydrate and Tris from Duchefa Biochemie (Haarlem, the Netherlands); the proteins holo-Trf, apo-Trf from bovine, albumin from human serum (HSA), ferritin from equine spleen, urease from jack beans, proteinase K from Engyodontium album and cytochrome c (cyt c) from equine heart were purchased from Sigma Aldrich. AT-cut QCM crystals (5 MHz) were purchased from Q-sense (Q-Sense AB, Sweden). All solutions were prepared with deionized water (DI water) obtained from a water purification system (Millipore, Eschborn, Germany).

Zhang X., Yarman A., Erdossy J., Katz S., Zebger I., Jetzschmann K.J., Altintas Z., Wollenberger U., Gyurcsányi R.E, & Scheller F.W. (2018). Electrosynthesized MIPs for transferrin: Plastibodies or nano-filters?. Biosensors & bioelectronics, 105.

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Electrochemical Analysis of Metal Salts

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Potassium chloride (99.0%), potassium bicarbonate (analytical quality), hydrochloric acid 36.5% V/V (ACS grade) were purchased from VWR analytical. Potassium hexacyanoferrate(II) trihydrate (98.5%), potassium formate (99%), and ferrocene methanol (97%) were purchased from Sigma Aldrich. Tin ingot (99.99%) was purchased from Alfa Aesar. Nitrogen gas (99.999%) and carbon dioxide (99.99%) gas cylinders were purchased from Praxair. All aqueous solutions were prepared using deionized water with a resistivity of 16.5 MΩ cm⁻¹.

Mayer F.D., Hosseini-Benhangi P., Sánchez-Sánchez C.M., Asselin E, & Gyenge E.L. (2020). Scanning electrochemical microscopy screening of CO2 electroreduction activities and product selectivities of catalyst arrays. Communications Chemistry, 3, 155.

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Preparation of Redox Electrolytes for Electrochemical Studies

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The [Fe(CN) 6 ] 3À/4À electrolyte was prepared from deionized water (Millipore, 418.2 MO cm), deuterium oxide (99.9% atom% D, Aldrich), potassium hexacyanoferrate(III) (499%, Alfa Aesar) and potassium hexacyanoferrate(II) trihydrate (99.5%, Sigma). Iron(III) chloride hexahydrate (498%, Sigma-Aldrich) and iron(II) chloride tetrahydrate (98%, Sigma-Aldrich) were used for the preparation of the [Fe(H 2 O) 6 ] 3+/2+ electrolyte. For cation dependent measurements, tetrabutylammonium chloride (TBACl, 498%, Sigma-Aldrich), tetraethylammonium chloride (TEACl 498%, Sigma-Aldrich), tetramethylammonium chloride (TMACl 498%, Sigma-Aldrich), lithium chloride (498%, Alfa Aesar), sodium chloride (99.99%, Alfa Aesar), potassium chloride (99.995%, Alfa Aesar), rubidium chloride (99.8%, Alfa Aesar), cesium chloride (99.9%, Alfa Aesar), lithium perchlorate (495%, Sigma-Aldrich), potassium perchlorate, sodium perchlorate (499%, Alfa Aesar), lithium nitrate (99%, Alfa Aesar), potassium nitrate (99%, Alfa Aesar), rubidium nitrate (99.7%, Sigma-Aldrich) and cesium nitrate (99.99%, Sigma-Aldrich) were used. Furthermore, both [Fe(CN) 6 ] 3À/4À and [Fe(H 2 O) 6 ] 3+/2+ redox couples were stable in the presence of perchlorate salts, as shown in the cyclic voltammograms (ESI2, †). This journal is © the Owner Societies 2018

Huang B., Muy S., Feng S., Katayama Y., Lu Y.C., Chen G, & Shao-Horn Y. (2018). Non-covalent interactions in electrochemical reactions and implications in clean energy applications. Physical chemistry chemical physics : PCCP, 20(23).

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Electrochemical Detection of Acetylcholinesterase

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Potassium hexacyanoferrate (III), potassium hexacyanoferrate (II) trihydrate, 25% GA, acetylcholine iodide (ATCh), acetylcholinesterase (AChE) from electric eel, nitric acid, and phosphate buffer saline (PBS) were purchased from Sigma‐Aldrich. Phosmet was obtained from J&K Scientific (Hong Kong) Ltd. Nb₂AlC (>99%) was obtained from Laizhou Kai Ceramic material Co., Ltd. Carbon fiber cloths (CFCs) were purchased from CeTech (W0S1002) with a thickness of 0.33 mm. The electrochemical characterizations were performed by using Solartron Electrochemical workstation with standard three electrode system in 1 m PBS (aq). All these chemicals were used as received without further purification.

Song M., Pang S., Guo F., Wong M, & Hao J. (2020). Fluoride‐Free 2D Niobium Carbide MXenes as Stable and Biocompatible Nanoplatforms for Electrochemical Biosensors with Ultrahigh Sensitivity. Advanced Science, 7(24), 2001546.

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