Apoptosis was detected using annexin V-fluorescein isothiocyanate (V-FITC)/ propidium iodide (PI) apoptosis kit (BD Bioscience, San Diego, CA, USA), cells transfected for 48 h were collected and added annexin V-FITC and PI. After incubation for 20 min in a dark condition, cell apoptosis was tested by flow cytometry (BD Bioscience).
Annexin 5 fluorescein isothiocyanate 5 fitc propidium iodide apoptosis kit
Manufactured by BD
Sourced in United States
The Annexin V-FITC/PI apoptosis kit is a laboratory tool used to detect and quantify apoptosis, a type of programmed cell death, in cells. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and propidium iodide, a DNA-binding dye, to distinguish between viable, apoptotic, and necrotic cells.
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3 protocols using annexin 5 fluorescein isothiocyanate 5 fitc propidium iodide apoptosis kit
1
Annexin V-FITC/PI Apoptosis Assay
2
Apoptosis Detection by Flow Cytometry
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Apoptosis was determined according to annexin V-fluorescein isothiocyanate (V-FITC)/propidium iodide (PI) apoptosis kit (BD Bioscience, San Diego, CA, USA) instructions. Cells were collected after transfection for 48 h and then added annexin V-FITC and PI. After incubation for 20 min in a dark condition, cell apoptosis was tested by flow cytometry (BD Bioscience, San Diego, CA, USA).
3
Annexin V-FITC/PI Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Apoptosis was determined by annexin V-fluorescein isothiocyanate (V-FITC)/propidium iodide (PI) apoptosis kit (BD Bioscience, San Diego, CA, USA) under the manufacturers' manuals. Cells were collected at 24 h after transfection and resuspended with binding buffer. Annexin V-FITC and PI were added to stain the cells for 20 min in a dark condition. Apoptosis was measured by flow cytometry (BD Bioscience).
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