Anti integrin β2

Histopaque 10771, Histopaque 11191, formaldehyde solution, and dimethylsulfoxide (DMSO, purity >99.5%), were purchased from Sigma-Aldrich (St. Louis, MO). Fluorescently-labeled antibodies, and antibodies including FITC-anti-CD11b and APC-anti-CD15 antibodies, monoclonal Anti-ICAM-1isotype, and enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-1β and IL-6, were purchased from Biolegend (San Diego, CA). Lipid staining dye DiO (3,3′-dihexadecyloxacarbocyanine perchlorate), DiD (1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine), Penicillin-streptomycin (pen-strep) and glutamine (100×) were purchased from Life Technologies (Grand Island, NY). Anti-ICAM-1, anti-integrin β₂, anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Pierce™ BCA protein assay kits and ECL Western blotting substrates were obtained from Thermo Fisher Scientific (Rockford, IL). RvD1 was purchased from Cayman Chemical (Ann arbor, MI). CEF was bought from TCI (Tokyo, Japan).