Transmission electron microscopy (TEM) observations were performed on a Tecnai F20 microscope. Atomic force microscope (AFM) measurements were carried out with Veeco Dimension 3100V. X-ray photoelectron spectroscopy (XPS) was carried out with ESCALAB 250Xi (Thermo Scientific, Waltham, MA, USA). Fourier transform infrared (FT-IR) spectra were obtained on a Nicolet 6700 FT-IR spectrometer (Thermo Nicolet Corp., Madison, WI, USA). Photoluminescence emission and excitation spectra were measured on a Hitachi F-4600 spectrophotometer (Hitachi, Tokyo, Japan) equipped with a Xe lamp at ambient conditions. UV-Vis absorption spectra were recorded on a PERSEE T10CS UV-Vis spectrophotometer (Persee, Beijing, China). PL lifetime was measured using Fluorolog 3-11 (HORIBA Jobin Yvon, Kyoto, Japan). PL Quantum yields were measured on a QE-2100 quantum efficiency measurement system (Otsuka Electronics, Japan). Photographs were taken using a Canon camera (EOS 550, Tokyo, Japan) under excitation by a hand-held UV lamp (365 nm).
Eos 550
Manufactured by Canon
Sourced in Japan
The EOS 550 is a digital camera designed for professional photography. It features a 24.2-megapixel full-frame CMOS sensor, DIGIC 8 image processor, and a 3.2-inch touchscreen LCD. The camera is capable of capturing high-quality images and 4K video.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (8 mentions)
F 4600 spectrophotometer, by Hitachi (4 mentions)
Fluorolog 3 11, by Horiba (2 mentions)
Dimension 3100v, by Veeco (1 mentions)
Tecnai f20 electron microscope, by Thermo Fisher Scientific (1 mentions)
Pma 12 spectrometer, by Hamamatsu Photonics (1 mentions)
Solidworks, by Dassault Systèmes (1 mentions)
D max2000, by Rigaku (1 mentions)
Fls980 photoluminescence spectrometer, by Edinburgh Instruments (1 mentions)
Tecnai f20 microscope, by Thermo Fisher Scientific (1 mentions)
6 protocols using eos 550
1
Comprehensive Characterization of Materials
2
Detailed Characterization of W-CDs Synthesis
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The W-CDs were prepared using a domestic microwave oven (Media). Transmission electron microscopy (TEM) images were acquired on a Tecnai F20 electron microscope (FEI Company, Hillsborough, OR, USA). XPS data were produced with an ESCALAB 250Xi (Thermo Scientific, Waltham, MA, USA). Fourier transform infrared (FT-IR) spectra were measured on a Nicolet 6700 FT-IR spectrometer (Thermo Nicolet Corp., Madison, WI, USA) with the KBr pellet technique. Fluorescence emission and excitation spectra were obtained on a Hitachi F-4600 spectrophotometer equipped with a Xe lamp (150 W) under ambient conditions. UV-Visible absorption spectra were obtained on a PERSEE T10CS UV-Vis spectrophotometer (Persee, Beijing, China). PL photographs were taken using a Canon camera (EOS 550) under excitation by a hand-held UV lamp (365 nm). PL lifetime was measured on a HORIBA FL3-111 fluorescence spectrometer (HORIBA Jobin Yvon, Kyoto, Japan). PL QY measurements were carried out with a QE-2100 quantum efficiency measurement system (Otsuka Electronics, Tokyo, Japan). The EL spectra and device parameters of W-LEDs were collected from a PMA-12 spectrometer (Hamamatsu Photonics, Hamamatsu, Japan) equipped with a fiber integration sphere.
3
Tympanic Membrane Dimensional Analysis
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The tympanal membrane was dissected from each animal after the laser vibrometry experiments. Photos were taken of each membrane through a dissecting microscope (Leica M80, Wetzlar, Germany) with a digital camera (Canon, EOS 550, Tokyo, Japan) and calibrated with a micrometre slide (Fine Science Tools, 29025-02, Heidelberg, Germany). The membranes were positioned as flat as possible, as any inclined angle would result in a skewed image for dimension analysis. Three photos were taken with different rotations to account for photo clarity and any slight offsets. Photos were then analysed using SolidWorks (Dassault Systemes, Waltham, MA, USA). Length was determined by measuring from the most posterior sclerotized portion to the furthest point on the curve of the tympanal membrane rim opposite (figure 1 d). Width was then determined from creating a line perpendicular to the length vector through the PV. Finally, the area of the PV was also measured. Measurements are mean ± s.e.m.
4
Comprehensive Characterization of Luminescent Materials
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Transmission electron microscopy (TEM) observations were performed on a Tecnai F20 microscope. X-ray photoelectron spectroscopy (XPS) spectra were carried out with ESCALAB 250Xi (Thermo Scientific). Scanning electron microscopy (SEM) was performed on a JEOL FESEM 6700F microscope with a primary electron energy of 3 kV. X-ray powder diffraction (XRD) patterns were recorded on a Rigaku D/max-2000 X-ray powder diffractometer (Japan) using Cu Kα (1.5406 Å) radiation. Fourier transform infrared (FT-IR) spectra were obtained on a Nicolet 6700 FT-IR spectrometer. Photoluminescence (PL), afterglow emission and excitation spectra were measured on a Hitachi F-4600 spectrophotometer at ambient conditions. For the temperature-dependent experiment, the sample was placed in a high temperature fluorescence attachment (Orient KOJI, TAP-02) with temperatures controlled between 298.15 and 523.15 K. UV–Vis absorption spectra were recorded on a PERSEE T10CS UV–Vis spectrophotometer. PL and afterglow lifetimes were measured using Fluorolog 3–11 (HORIBA Jobin Yvon). PL quantum yields (QYs) were measured on a QE-2100 quantum efficiency measurement system (Japan Otsuka Electronics). Photographs of PL and afterglow were taken using a Canon camera (EOS 550) under excitation by a hand-hold UV or LED lamps.
5
Comprehensive Characterization of Luminescent Materials
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Transmission electron microscopy (TEM) observations were performed on a Tecnai F20 microscope (FEI, Hillsboro, OR, USA). X-ray photoelectron spectroscopy (XPS) was carried out with ESCALAB 250Xi (Thermo Scientific, Waltham, MA, USA). Fourier transform infrared (FT-IR) spectra were obtained on a Nicolet 6700 FT-IR spectrometer (Thermo Nicolet Corp., Madison, WI, USA). Photoluminescence, phosphorescence emission and excitation spectra were measured on a Hitachi F-4600 spectrophotometer (Hitachi, Tokyo, Japan) at ambient conditions. UV-Vis absorption spectra were recorded on a PERSEE T10CS UV-Vis spectrophotometer (Persee, Beijing, China). FL and Phos lifetimes were measured using an EDINBURGH FLS 980photoluminescence spectrometer (Edinburgh Instruments, Wales, UK). Photographs of FL and Phos images were taken using a Canon camera (EOS 550, Tokyo, Japan) under a hand-held blue LED lamp (420 nm). PL QY measurements were carried out with a QE-2100 quantum efficiency measurement system (Otsuka Electronics, Tokyo, Japan).All the FL and RTP spectra and lifetimes were measured under aerobic atmosphere in this study unless otherwise noted.
6
Reproductive System Morphology and GSI Analysis
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All fish were euthanized by rapid cooling shock [8] . Their abdominal cavities were then longitudinally dissected and the reproductive systems (ovary and reproductive ducts) were morphologically observed for visual characters including position, shape and colorization, which were photographed using a digital camera (Canon EOS 550). Subsequently, each whole gonad was weighted to an accuracy of 0.01 g [9] for calculating the gonadosomatic indices (GSI) using the formula GSI = [ovarian weight/total weight×100]. This gravimetric valve was used for the determination of gonadal development/maturation [10, 11] .
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