Automatic clinical chemistry analyzer

Data on baseline characteristics, including age, sex, body mass index (BMI), and smoking status, were collected. Total, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) cholesterol levels and triglyceride levels were measured using colorimetric assays (Hitachi LST008, Automatic Clinical Chemistry Analyzer, Hitachi, Naka, Japan). Non-HDL and remnant cholesterol levels were calculated by subtracting HDL from total cholesterol levels and by subtracting HDL and LDL from total cholesterol levels, respectively [45 (link)]. The definition of metabolic syndrome is shown in the Supplementary Materials, and other metabolic traits are shown in Supplementary Table S7 and as previously reported [41 (link),42 (link)].

Rats were anaesthetized with ketamine-xylazine at the end of the experiment, and blood samples were collected via cardiac puncture for hematology and serum biochemistry analyses. Blood serum for biochemistry analysis was collected in non-heparinized tubes. Collected blood was left at room temperature for a while to allow clotting before serum collection by centrifugation (3000× g for 10 min) at 4 °C. The collected serum was stored at −4 °C before analysis using an automatic clinical chemistry analyzer (Hitachi, Japan). The parameters analyzed for serum biochemistry analysis were as follows: albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine, total bilirubin, urea, and total protein.
Blood was collected in K2EDTA tubes using an automated hematology analyzer (CELL-- DYN 3700 Abbott Diagnostics, Des Plaines, IL, USA). Parameters analyzed for whole blood cells were as follows: red blood cell count, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concentration, thrombocytes, and white blood cell count (including lymphocytes, monocytes, neutrophils, and eosinophils) [24 (link)].

At the end of the experiment, animals were anesthetized with diethyl-ether, killed by cardiac puncture and blood was collected for hematology and serum biochemistry analyses. For hematological study, blood was collected in tubes containing K2EDTA and analyzed using an automated hematological analyzer (CELL-DYN 3700 Abbott Diagnostics, USA) for the following parameters: red blood cell count, hemoglobin, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin concentration, thrombocytes and white blood cell count (including lymphocytes, monocytes, neutrophils and eosinophils).
For serum biochemistry analysis, blood was collected in non-heparinized blood collection tubes, allowed to clot at room temperature, and centrifuged at 3000 × g for 10 min at 4°C after which serum was collected. Serum biochemistry was analyzed using an automatic clinical chemistry analyzer (Hitachi, Japan) for the following parameters: albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, total cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, glucose, creatinine, total bilirubin, calcium, inorganic phosphorus, urea, total protein and electrolytes, including sodium, potassium and chloride.