Sb218795

Manufactured by Merck Group

SB218795 is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and analysis. The core function of SB218795 is to provide a reliable and precise measurement tool for researchers.

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Lab products found in correlation

2 protocols using sb218795

Pharmacological Compound Preparation

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Drugs were purchased from either Sigma-Chemical Company (ARL67156, ATP, adenosine, EHNA, TNP-ATP, suramin, ADPβS, PPADS, apyrase, hexamethonium, adenosine deaminase, NF279) or Tocris (α,β-Me-ATP, MRS1220, MRS2179, SB218795) and 1–10mM stocks were dissolved in distilled water or DMSO (final concentration ≤0.1%) and diluted into Krebs buffer. Drugs > 0.2mM concentrations were adjusted for pH if needed.

Liñán-Rico A., Wunderlich J., Enneking J., Tso D., Grants I., Williams K., Otey A., Michel K., Schemann M., Needleman B., Harzman A, & Christofi F. (2015). Neuropharmacology of Purinergic Receptors in Human Submucous Plexus: Involvement of P2X1, P2X2, P2X3 Channels, P2Y and A3 Metabotropic Receptors in Neurotransmission. Neuropharmacology, 95, 83-99.

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Enteric Glia Modulate Colonic Tachykininergic Contractions

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The first set of experiments was performed to examine the role of enteric glia in colonic tachykininergic NK₁-mediated contractions. Contractions were recorded from colonic preparations maintained in Krebs solution containing 100 μM N^ω-nitro-L-arginine methylester (L-NAME), 10 μM guanethidine, 1 μM atropine, 1 μM GR159897 (NK₂ receptor antagonist), and 1 μM SB218795 (NK₃ receptor antagonist), either in the absence or presence of the selective gliotoxin fluorocitrate (FC, 50 µΜ, Sigma-Aldrich F9634). FC is widely used to inhibit the tricarboxylic acid enzyme aconitase, determining the pharmacological blockade of glial cells through an alteration of their energy-dependent processes [22 (link),23 (link)].
The second and third series aimed at investigating the effects of BAY60-6583 (A_2BR agonist, Sigma-Aldrich SML1958) and MRS1754 (A_2BR antagonist, Tocris 2752) in the absence or presence of FC. Electrically evoked contractions were recorded from colonic preparations maintained in Krebs solution containing 100 μM L-NAME, 10 μM guanethidine, 1 μM atropine, 1 μM GR159897, 1 μM SB218795, and 1 μM BAY60-6583 or 10 nM MRS1754, either in the absence or presence of 50 µΜ FC. Concentrations were selected in accordance with previous studies [9 (link),21 (link)].

D’Antongiovanni V., Benvenuti L., Fornai M., Pellegrini C., van den Wijngaard R., Cerantola S., Giron M.C., Caputi V., Colucci R., Haskó G., Németh Z.H., Blandizzi C, & Antonioli L. (2020). Glial A2B Adenosine Receptors Modulate Abnormal Tachykininergic Responses and Prevent Enteric Inflammation Associated with High Fat Diet-Induced Obesity. Cells, 9(5), 1245.

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