We used a previously-described minimally invasive carbon fiber electrode array 15 (link) in addition to commercially available arrays (TDT, Neuronexus) to chronically monitor both single units and LFPs. Extracellular voltages were amplified and digitized at either 25 or 30 Khz using the Intan acquisition system (RHA2000 and RHD2000).
Rha2000
Manufactured by Intan Technologies
Sourced in United States
The RHA2000 is a laboratory equipment designed for scientific research and analysis. It is a high-performance, multi-purpose instrument that can be used in a variety of applications. The core function of the RHA2000 is to provide accurate and reliable measurements and data analysis. The specific details of its intended use and capabilities are not available in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using rha2000
1
Chronic Neuronal Monitoring with Electrodes
2
Extracellular Signaling Analysis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
One week after surgery, experiments began with a 1 h recording session in the plethysmograph (see below) or in a custom-built head-fixed setup with a treadmill for voluntary running [9 (link)]. Extracellular signals were filtered (1–500 Hz), amplified (RHA2000, Intan Technologies, Los Angeles, USA), digitized (2.5 kHz), and stored for offline analysis. A three-dimensional accelerometer mounted on the amplifier board at the animals head allowed movement detection. Mice habituated quickly to the head-restraining conditions and tolerated it well for up to 1 h. The whole-body plethysmograph (PG) consisted of a transparent cylindrical box (78 mm inner diameter, 165 mm height) which was connected to a reference chamber and adapted for collection of LFP (EMKA Technologies, SAS, France). Mice habituated well to the PG apparatus within the first session.
3
Chronic Neuronal Monitoring with Electrodes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used a previously-described minimally invasive carbon fiber electrode array 15 (link) in addition to commercially available arrays (TDT, Neuronexus) to chronically monitor both single units and LFPs. Extracellular voltages were amplified and digitized at either 25 or 30 Khz using the Intan acquisition system (RHA2000 and RHD2000).
4
Simultaneous Electrophysiology and Movement Monitoring in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were placed in a whole-body plethysmograph (EMKA Technologies, SAS, France), which was customized to allow simultaneous recordings of local field potentials (LFPs). It consisted of a transparent cylinder (78 mm inner diameter, 165 mm height) connected to a reference chamber (Figure 1A). Individual recording sessions lasted from 2 to 6 hours (mean: 3 h). Monopolar electrophysiological signals were filtered (1-500 Hz), amplified and digitized at 2.5 kHz (RHA2000, Intan Technologies, Los Angeles, USA). A threedimensional accelerometer was custom-mounted on the amplifier board located on the head of the mice to allow movement detection. The three signals of the accelerometer were fed to three channels of the amplifier using the same band-pass filter as for the LFPs (see above), therefore removing the gravity-induced sustained potentials of the accelerometers.
REM-sleep was manually staged by a senior researcher in the field (J.B.) and identified as follows: 1) minimal accelerometer activity and 2) continuous theta rhythm in the parietal cortex following a sleep stage with slow-waves typical for non-REM sleep (Brankačk et al., 2010) .
REM-sleep was manually staged by a senior researcher in the field (J.B.) and identified as follows: 1) minimal accelerometer activity and 2) continuous theta rhythm in the parietal cortex following a sleep stage with slow-waves typical for non-REM sleep (Brankačk et al., 2010) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!