Disposable capillary cell

Manufactured by Malvern Panalytical

Sourced in United Kingdom

Disposable capillary cells are single-use laboratory equipment designed for various analytical techniques. They provide a contained environment for samples during measurement or testing processes. The core function of these cells is to hold and present the sample in a controlled manner for analysis.

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Lab products found in correlation

Nano z, by Malvern Panalytical (6 mentions) Zetasizer nano z, by Malvern Panalytical (4 mentions) Disposable square polystyrene cuvettes, by Malvern Panalytical (2 mentions) Disposable cuvette, by Sarstedt (2 mentions) Sputter coated 180aute, by Cressington (2 mentions) Xl30 esem feg, by Thermo Fisher Scientific (2 mentions) Malvern zetasizer nano z, by Malvern Panalytical (1 mentions) Zetasizer, by Malvern Panalytical (1 mentions) Nano zs zetasizer, by Malvern Panalytical (1 mentions) Zetasizer nano zs90, by Malvern Panalytical (1 mentions)

15 protocols using disposable capillary cell

Characterization of LNC Suspensions

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Size distribution, polydispersity index (PDI), and zeta potential measurements of LNC suspensions were determined by photon-correlation spectroscopy using a Zetasizer® Nano ZS (Malvern Instruments, Northampton, UK). 20 μL of LNC suspension was diluted to 1000 μL of purified water and added in disposable capillary cells (Malvern Instruments, Northampton, UK). Measurements were conducted in triplicate at 25°C. The helium-neon laser operates at 663 nm with the scatter angle fixed at 173°. The autocorrelation function was modeled using the NonNegative Least Squares algorithm.
For the stability study, LNC low and blank LNC were divided into three groups immediately after preparation and stored at 4°C, 25°C, and 37°C. Samples were tested at day 0, 7, 14, and 28 for size distribution and PDI measurements as described above.

Chouchou A., Aubert-Pouëssel A., Dorandeu C., Zghaib Z., Cuq P., Devoisselle J.M., Bonnet P.A., Bégu S, & Deleuze-Masquefa C. (2017). Lipid nanocapsules formulation and cellular activities evaluation of a promising anticancer agent: EAPB0503. International Journal of Pharmaceutical Investigation, 7(4), 155-163.

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Nanocarrier Physicochemical Characterization

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The hydrodynamic size (Z-Average), polydispersity index (PDI) and Zeta potential of the NC were determined by NanoZS (Malvern Instrument, UK) using disposable square polystyrene cuvettes (Malvern Instrument, UK) for size and PDI, or disposable capillary cells (Malvern Instrument, UK) for Zeta potential at 25°C. For size measurement, NC samples were diluted with deionised water. The Z-Average diameter and polydispersity index were presented as the average value of three measurements, with 15 runs within each measurement. Electrophoretic mobility was used to calculate the Zeta potential measurement. NC samples were diluted with deionised water. Three measurements were performed with 20-25 runs within each measurement. The mean and standard deviation of size and Zeta potential were calculated for each sample.

Bai J., Wang J.T., Rubio N., Protti A., Heidari H., Elgogary R., Southern P., Al-Jamal W.T., Sosabowski J., Shah A.M., Bals S., Pankhurst Q.A, & Al-Jamal K.T. (2016). Triple-Modal Imaging of Magnetically-Targeted Nanocapsules in Solid Tumours In Vivo. Theranostics, 6(3), 342-356.

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Polyplexes Characterization by DLS and Zeta Potential

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Sizes of
the polyplexes were evaluated by dynamic light-scattering (DLS) analysis.
Polyplexes were prepared with 40 pmol of FLUC siRNA at N/P ratios
of 2 and 10, as described above, in a total volume of 350 μL.
Measurements were performed with a Zetasizer Nano ZS (Malvern Instruments
Inc., Westborough, MA) in quadruplicate at 25 °C using disposable
cuvettes (low volume 70 μL, Brookhaven Instruments Corporation,
Holtsville, NY) for size measurements. Measurements were set up at
a 173° backscatter angle with 15 runs per measurement. For data
analysis, the viscosity (0.88 mPa s) and the refractive index (1.33)
of water at 25 °C were used. Results are given as Z average in nanometers ± standard deviation. Polyplexes were
then diluted to 700 μL with a 5% glucose solution before ζ-potential
measurements were performed in disposable capillary cells (Malvern
Instruments Inc.). Results are given in millivolts ± standard
deviation.

Elsayed M., Corrand V., Kolhatkar V., Xie Y., Kim N.H., Kolhatkar R, & Merkel O.M. (2014). Influence of Oligospermines Architecture on Their Suitability for siRNA Delivery. Biomacromolecules, 15(4), 1299-1310.

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Liposome Characterization: Size, Polydispersity, and Charge

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The hydrodynamic diameter, polydispersity index and zeta potential of the liposomes were measured using the NanoZS (Malvern Instrument, UK). Hydrodynamic size, polydispersity index (PDI) and zeta potential were measured in disposable square polystyrene cuvettes and disposable capillary cells, respectively (Malvern Instrument, UK). The sample (20 µl) was diluted to 1.5 ml with 10 mM sodium chloride. The measurements were carried out at room temperature. Three measurements were performed, and the mean and standard deviation were calculated for each sample.

Wang J.T., Hodgins N.O., Al-Jamal W.T., Maher J., Sosabowski J.K, & Al-Jamal K.T. (2020). Organ Biodistribution of Radiolabelled γδ T Cells Following Liposomal Alendronate Administration in Different Mouse Tumour Models. Nanotheranostics, 4(2), 71-82.

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Nanoparticle Characterization Techniques

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The hydrodynamic size (Z-Average), polydispersity index (PDI) and zeta potential of NCs and m-NCs were determined by NanoZS (Malvern Instrument, UK) at 25 °C using disposable square polystyrene cuvettes (for size and PDI) or disposable capillary cells (for zeta potential) (Malvern Instrument, UK). The Z-Average diameter and polydispersity index were measured in water and presented as the average value of three measurements, with 15 runs within each measurement. The zeta potential was also measured in water and presented as the average value of three measurements, with 20–25 runs within each measurement. The mean and standard deviation (SD) of size and zeta potential were calculated for each sample.

Bai J., Wang J.T., Mei K.C., Al-Jamal W.T, & Al-Jamal K.T. (2016). Real-time monitoring of magnetic drug targeting using fibered confocal fluorescence microscopy. Journal of Controlled Release, 244(Pt B), 240-246.

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Characterization of Fluorescent Silica Nanoparticles

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Green
fluorescently labeled silica nanoparticles (maximum excitation 485
nm and emission 510 nm) of 100 and 200 nm diameter, and with a plain,
amino-modified or carboxylated surface (SiO₂, SiO₂–NH₂, and SiO₂–COOH, respectively),
were purchased from Micromod Partikeltechnologie GmbH (Rostock, Germany).
All amino and carboxylated nanoparticles had a surface charge density
of 1 μmol g^–1, except for 200 nm SiO₂–NH₂, which had a surface charge density of 4 μmol
g^–1. The same batch of nanoparticles was used for
all studies, with the exception of the 200 nm SiO₂–NH₂ results shown in Supporting Figures S5 and S6 and one of the 3 experiment repeats shown in Figure 5a,b. A Malvern Zetasizer
Nano ZS (Malvern Instrument Ltd., Worcestershire, UK) was used for
nanoparticle characterization by dynamic light scattering (DLS) to
obtain the nanoparticle size distribution and for zeta potential (ζ-potential)
measurements. Briefly, dispersions of the nanoparticles or of the
corona-coated nanoparticles (50 μg mL^–1 and
30 μg mL^–1, for 100 and 200 nm nanoparticles,
respectively) in PBS were measured at 20 °C in disposable capillary
cells (Malvern) just after preparation. For each sample, measurements
were repeated at least 3 times with 5 runs each.

Aliyandi A., Reker-Smit C., Bron R., Zuhorn I.S, & Salvati A. (2021). Correlating Corona Composition and Cell Uptake to Identify Proteins Affecting Nanoparticle Entry into Endothelial Cells. ACS Biomaterials Science & Engineering, 7(12), 5573-5584.

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Electrophoretic Zeta-Potential Analysis of C. albicans

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Zeta-potential analyses were performed using electrophoretic light scattering on C. albicans 39343 at pH 5, 7 and 9 and at a salt concentration of 0.01 mol l -1 NaCl. C. albicans was grown overnight in SAB at 37°C, and diluted 100-fold in fresh SAB prior to culture at 37°C for 19 h at 80 rev min -1 . One ml of the culture was washed in distilled-water (5,500 x g; 3 min). The pellet was re-suspended in 100 µl of 0.01 mol l -1 NaCl (pH 5, 7 or 9) and 20 µl added to 1 ml of the buffer ± OligoG (10%) for 20 min; the sample was washed and centrifuged (2,500 x g; 6 min). A Zetasizer Nano ZS (Malvern Instruments) and disposable capillary cells (DTS1061 Malvern instruments) were employed and the resultant zeta potential calculated by applying Smoluchowski's model (Wilson et al. 2001) .

Pritchard M.F., Jack A.A., Powell L.C., Sadh H., Rye P.D., Hill K.E, & Thomas D.W. (2017). Alginate oligosaccharides modify hyphal infiltration of Candida albicans in an in vitro model of invasive human candidosis. Journal of applied microbiology, 123(3).

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Particle Size and Zeta Potential Analysis

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Hydrodynamic diameters of dialysed dispersions were measured by Zetasizer SZ (Malvern). 50 l of the samples were diluted in 400 l of PBS in 1 ml cuvette to obtain attenuation values in the 7-9 range and measured at 25 • C equilibrating samples for 120 s prior to measurement. Data were presented as an average of three measurements. The Z-averaged sizes (Z-ave) and the polydispersity index (PdI) were obtained by cumulant analysis of the auto-correlation functions. Zeta potential (Zp) was measured using disposable capillary cells (Malvern). Data are the average of three measurements of 10 runs each.

Di Silvio D., Rigby N., Bajka B., Mackie A, & Baldelli Bombelli F. (2016). Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake. The international journal of biochemistry & cell biology, 75.

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Nanoparticle Characterization via Zetasizer

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Prior to freezing and lyophilizing, as well as post, particle size and zeta potential were analyzed via a Malvern Zetasizer Nano ZS. Briefly, a small amount of lyophilized NPs was re-suspended in 1 mL DDI H₂O, vortexed for 30 seconds, then transferred to a disposable cuvette (Sarstedt) for analysis of particle size (in nm) by dynamic light scattering. The sample was then transferred to a disposable capillary cell (Malvern) for analysis of zeta potential. For each NP batch, the mean diameter ± S.D. for three measurements was determined. The polydispersity index (PDI) was also quantified to establish particle size distribution.

Castañeda-Gill J., Ranjan A, & Vishwanatha J. (2017). Development and Characterization of Methylene Blue Oleate Salt-Loaded Polymeric Nanoparticles and their Potential Application as a Treatment for Glioblastoma. Journal of nanomedicine & nanotechnology, 8(4), 449.

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Characterization of PLGA Nanoparticles

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NPs were sized using both imaging techniques (SEM) and dynamic light scattering (DLS). For SEM imaging, dry PLGA BPNPs (without trehalose) were placed on carbon tape and sputter coated with gold for 30 sec under 40 mA current (Sputter Coated 180aute, Cressington). Images were acquired using a XL-30 ESEM-FEG (FEI Company, Hillsboro, OR, USA) under 10 kV acceleration voltage. Average nanoparticle size was quantified using the ImageJ software (National Institutes of Health, Bethesda, MD, USA), where sample populations of at least 1,000 NPs were included for statistical analysis.
Hydrodynamic size analysis was completed using DLS. BPNPs were resuspended at 0.05 mg/mL in DI water and sized using a Malvern Nano-ZS. Results are reported as the Z-average diameter, corresponding to the hydrodynamic diameter of the particles. In all cases, the polydispersity index was less than 0.2. To measure surface charge (zeta potential), BPNPs were diluted in DI water at a concentration of 0.5 mg/mL; 750 μL of solution was loaded into a disposable capillary cell (Malvern, UK) and the charge was measured using a Malvern Nano-ZS.

Saucier-Sawyer J.K., Seo Y.E., Gaudin A., Quijano E., Song E., Sawyer A.J., Deng Y., Huttner A, & Saltzman W.M. (2016). Distribution of Polymer Nanoparticles by Convection-Enhanced Delivery to Brain Tumors. Journal of controlled release : official journal of the Controlled Release Society, 232, 103-112.

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