Anti acp5

Lung tissues and fibroblasts were homogenized in RIPA lysis buffer (Beyotime, Shanghai, China). For subcellular fractionation, the protein was extracted using NE-PER^TM Nuclear and Cytoplasmic Extraction reagents (Thermo Fisher Scientific, USA). Western blot was performed according to previously reported protocols^{48 (link)}. The primary antibodies used were anti-ACP5 (Gentex, CA, USA, 1:1,000); anti-LAMIN B1, anti-FIBRONECTIN and anti-COL1A1 (Proteintech, Wuhan, China, 1:1,000); anti-α-SMA, antiphospho-β-CATENIN (Ser33/37/Thr41, Cell Signaling Technology, MA, USA, 1: 1000), and anti-β-CATENIN (Cell Signaling Technology, MA, USA, 1: 1000); and anti-β-ACTIN (Abcam, MA, USA, 1:3000). β-ACTIN bands were used as the loading control for cytoplasmic or total proteins, and LAMIN B1 bands were used as the loading control for nuclear proteins. Detection was performed using a chemiluminescent substrate system (Bio-Rad Laboratories, CA, USA). The gray values were analyzed with ImageJ software.