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Sputter coater

Manufactured by Cressington
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The Sputter Coater is a piece of lab equipment used to deposit thin, uniform coatings of conductive materials onto the surface of a sample. It operates by creating a plasma and sputtering atoms from a target material, which are then deposited onto the sample.

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Lab products found in correlation

Nova nanosem 230, by Thermo Fisher Scientific (2 mentions) Evo 18, by Zeiss (2 mentions) Su3500, by Hitachi (2 mentions) Gemini 2 column, by Zeiss (1 mentions) Merlin sem, by Zeiss (1 mentions) Nova nanosem 400, by Thermo Fisher Scientific (1 mentions) Tap300al g, by Ted Pella (1 mentions) S 4700 sem, by Hitachi (1 mentions) Sp5 x confocal microscope, by Leica (1 mentions) Supra 40, by Zeiss (1 mentions) Carbon coater, by Cressington (1 mentions) Vegaii microscope, by TESCAN (1 mentions) Supra 40vp, by Zeiss (1 mentions) Auriga compact, by Zeiss (1 mentions) Tabletop sem tm3030plus, by Hitachi (1 mentions) Cpd 030, by Cressington (1 mentions) Xl30 esem feg environmental, by Philips (1 mentions) H 7650, by Hitachi (1 mentions) Osmium tetroxide, by Merck Group (1 mentions) S 2700 scanning electron microscope, by Hitachi (1 mentions)

23 protocols using sputter coater

1

Morphology Analysis of Microparticles using SEM

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Scanning electron microscopy (SEM) was used to investigate morphology of MPs (Zeiss Merlin SEM, GEMINI II column). MPs were resuspended in DI water and a droplet was added to a piece of conductive tape attached to a SEM stub. The water was evaporated overnight, and the MPs were sputter coated with gold for 70 s (Cressington Sputter Coater) before imaging.
DeJulius C.R., Bernardo-Colón A., Naguib S., Backstrom J.R., Kavanaugh T., Gupta M.K., Duvall C.L, & Rex T.S. (2020). Microsphere antioxidant and sustained erythropoietin-R76E release functions cooperate to reduce traumatic optic neuropathy. Journal of controlled release : official journal of the Controlled Release Society, 329, 762-773.
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2

Cecal Tissue Preparation for SEM

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Cecal tissues were fixed in 3% glutaraldehyde buffered with 0.1 M phosphate buffer, pH 7.2, washed with 0.1 M phosphate buffer, and dehydrated in a graded ethanol solution in water (30% increased gradually to 100%; 20 min each). The samples were dried with a Leica CPD300 critical point dryer and coated with Pt(80)/Pd (20 (link)) of an 8- to 12-nm thickness by using a Cressington sputter coater (model 208HR). Samples were viewed with a FEI Nova NanoSEM230 scanning electron microscope (SEM).
Zaborin A., Krezalek M., Hyoju S., Defazio J.R., Setia N., Belogortseva N., Bindokas V.P., Guo Q., Zaborina O, & Alverdy J.C. (2016). Critical role of microbiota within cecal crypts on the regenerative capacity of the intestinal epithelium following surgical stress. American Journal of Physiology - Gastrointestinal and Liver Physiology, 312(2), G112-G122.
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3

Analyzing Extrudate Morphology and Protein Distribution

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A scanning electron microscope (SU 3500, Hitachi High Technologies, Krefeld, Germany), equipped with a backscattered electron detector (BSE) was used to investigate the morphology of the surfaces and cross sections of the prepared extrudates. BSE images were collected at an acceleration voltage of 5 kV at a variable pressure mode of 30 Pa. The cut extrudates were placed on an aluminum stub. Samples were sputtered with a thin platin layer (Sputter Coater, Cressington Scientific Instruments, Watford, England). Protein particle distribution was examined by elemental mapping of the cross sections of extrudates for the characteristic X-ray peak of nitrogen. The elemental distributions were investigated by SEM combined with an energy-dispersive X-ray detector (EDX) (EDAX Element-C2B, Ametek, Weiterstadt, Germany). The percentage of detected nitrogen was evaluated by the TEAM software (Version 4.4.1, Ametek, Weiterstadt, Germany).
Dauer K., Kayser K., Ellwanger F., Overbeck A., Kwade A., Karbstein H.P, & Wagner K.G. (2023). Highly protein-loaded melt extrudates produced by small-scale ram and twin-screw extrusion - evaluation of extrusion process design on protein stability by experimental and numerical approaches. International Journal of Pharmaceutics: X, 6, 100196.
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4

Characterizing Nanofiber Morphology via SEM

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The morphology and the size of
the nanofibers were assessed by using a scanning electron microscope
(SEM) (NovaNanoSEM 230, FEI). Samples of the nanofibrous mats were
harvested and coated with platinum–iridium (Pt–Ir) using
a sputter coater (Cressington). After Pt–Ir coating, the samples
were examined with an accelerated voltage of 5 kV–8 kV. The
diameter of the nanofibers was inferred by evaluating the SEM images
obtained using ImageJ.
Serpelloni S., Williams M.E., Caserta S., Sharma S., Rahimi M, & Taraballi F. (2024). Electrospun Chitosan-Based Nanofibrous Coating for the Local and Sustained Release of Vancomycin. ACS Omega, 9(10), 11701-11717.
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5

Scanning Electron Microscopy of Starch Granules

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Starch samples were spread onto a circular metal stub covered with double-sided adhesive tape and were coated with platinum by a sputter coater (Cressington, UK). Images of starch granules were acquired using a scanning electron microscope (SEM, model EVO 18, Zeiss, Germany) under an accelerating voltage of 10 kV and at multiple magnifications [28 (link)].
Hu Z., Zhao L., Hu Z, & Wang K. (2018). Hierarchical Structure, Gelatinization, and Digestion Characteristics of Starch from Longan (Dimocarpus longan Lour.) Seeds. Molecules, 23(12), 3262.
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6

Ultrastructural Analysis of Tetrahymena

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Tetrahymena samples collected from different time points of regrowth after deciliation were fixed by 1% glutaraldehyde and placed in pre-processed aluminum carriers from the M. Wohlwend GmbH, Engineering office. Before adding Tetrahymena cells, the carriers were carbon coated and glow discharged (two minutes) followed by treatment of poly-L-lysine coating to increase cell attachment. The Tetrahymena cells in the carriers were subjected to a six-step gradient dehydration of ethyl alcohol (10%, 20%, 40%, 70%, 90%, and 100%) followed by critical-point drying using Samdri-795 with a 15 minutes purging process. Afterwards, the samples in the carrier were fixed on conductive stubs with silver glue and gold-coated for 45 seconds using a CRESSINGTON Sputter Coater. SEM images were captured at 2 kx, 5 kx, and 10 kx magnifications using Secondary Electrons Secondary Ions detector of a ZEISS NEON 40EsB High Resolution Dual Beam Scanning Electron Microscope.
Reynolds M.J., Phetruen T., Fisher R.L., Chen K., Pentecost B.T., Gomez G., Ounjai P, & Sui H. (2018). The Developmental Process of the Growing Motile Ciliary Tip Region. Scientific Reports, 8, 7977.
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7

Neural Recording Microelectrode Array Protocol

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Ionic liquid 1-Ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl) imide (EMIIM) was acquired from Covalent Associates, Inc. (OR). All other chemicals were obtained from Sigma-Aldrich (MO). 22 mm plastic coverslips (Fisher, PA) were cut to uniform dimension of 7×22 mm, cleaned with 8N HNO3 for 30 min, washed in deionized H2O and stored in ethanol. Coverslips were then sputter coated with 35nm of gold, using a Cressington Sputter Coater (Cressington, UK). 32 channel gold site microelectrode arrays for in vivo neural recording were generously provided by Science and Biomedical Microsystems (MD). The GSA of electrode sites on the probe was 155 μm2. Each of the 3 shanks that were 150 μm apart had 10 sites arranged in an arrowhead formation; with one site at the tip of the shank and the rest in two parallel columns. The middle shank also has 2 sites at least 200 μm away from the rest. They were not included in the neurophysiology experiments. The adjacent sites center to center distance is 25 um and the center to center distance of the furthest sites was 135 μm. The densely packed sites ensured all recording were performed within the same layer of cortex.
Du Z.J., Luo X., Weaver C, & Cui X.T. (2015). Poly (3, 4-ethylenedioxythiophene)-ionic liquid coating improves neural recording and stimulation functionality of MEAs. Journal of materials chemistry. C, Materials for optical and electronic devices, 3(25), 6515-6524.
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8

Scanning Electron Microscopy of Quinoa Starch

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Quinoa starch samples isolated by different extraction methods were pasted on an adhesive carbon tape followed by platinum coating with a sputter coater (Cressington Scientific Instruments, Cressington, UK). Sample scanning was performed at an accelerated voltage of 10 kV using the SEM (Carl ZEISS, EVO 18, Oberkochen, Germany) and images were snapped at 500×, 1000×, and 10,000× magnifications.
Junejo S.A., Wang J., Liu Y., Jia R., Zhou Y, & Li S. (2022). Multi-Scale Structures and Functional Properties of Quinoa Starch Extracted by Alkali, Wet-Milling, and Enzymatic Methods. Foods, 11(17), 2625.
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9

Characterizing PLGA Nanoparticle Morphology

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The morphology of the PLGA NPs were determined by scanning electron microscopy (SEM). Briefly, dried PLGA/OVA NPs and PLGA/OVA/MPLA NPs were applied onto a pin stub covered with double-sided carbon tape and then coated with 3.5 nm of platinum/palladium using a Cressington Sputter Coater. Images were taken using an FEI Quanta 200F SEM (Franceschi Microscopy and Imaging center, WSU, WA) at 5 to 10 kV accelerating voltage. The PLGA NP preparations sizes were measured using ImageJ software (NIH, 1.48v).
Guldner D., Hwang J.K., Cardieri M.C., Eren M., Ziaei P., Norton M.G, & Souza C.D. (2016). In Vitro Evaluation of the Biological Responses of Canine Macrophages Challenged with PLGA Nanoparticles Containing Monophosphoryl Lipid A. PLoS ONE, 11(11), e0165477.
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10

Biofilm Characterization in Stainless Steel

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To examine biofilms inside the small stainless-steel tubes using SEM, some preparations were required; these preparations were executed as described previously (Speers et al., 1984 (link); Latorre et al., 2010 (link)) with some modifications. The tubes, which were taken from the fouling system, were rinsed with 10 mL of 0.1% peptone water and then placed in glass vials. The tubes were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer at pH 7.4 in glucose solution. The tubes were rinsed three times in 0.1 M phosphate buffer for 15 min each, and each tube was then cut carefully into halves longitudinally. The half-tube pieces were rinsed three times in 0.1 M phosphate buffer, 15 min each rinse. The washed biofilm-containing half tubes were dehydrated in increasing concentrations of ethanol (25, 50, 70, 85 and 95%) for 10 min each, and then three times in 100% ethanol for 30 min each. Dried samples were mounted onto aluminum stubs and coated with gold for 2 min in a sputter coater (Cressington, Redding, CA). The samples were imaged by SEM (Nova 400 NanoSEM, FEI, Hillsboro, OR).
Tirpanci Sivri G., Abdelhamid A.G., Kasler D.R, & Yousef A.E. (2023). Removal of Pseudomonas fluorescens biofilms from pilot-scale food processing equipment using ozone-assisted cleaning-in-place. Frontiers in Microbiology, 14, 1141907.
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