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Anti cd14 monoclonal antibody

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The Anti-CD14 monoclonal antibody is a laboratory reagent used for the detection and characterization of CD14, a protein expressed on the surface of certain immune cells. This antibody can be used in various immunoassay techniques to identify and analyze CD14-positive cells.

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Lab products found in correlation

Mcp 1, by Thermo Fisher Scientific (2 mentions)

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Anti-CD14 antibody (14 citations) CD14 antibody (3 citations)

2 protocols using anti cd14 monoclonal antibody

1

Calcium Mobilization in Leukocyte Activation

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Activation of human neutrophils and monocytes was performed by real-time detection of calcium mobilization using flow cytometry18 (link). Human monocytes were labeled with an anti-CD14 monoclonal antibody (BD). Cells, labeled with Fluo-3AM, were pre-incubated with HlgA or HlgC for 1 min at room temperature. Subsequently, baseline fluorescence was recorded in a flow cytometer. After 10 s of baseline recording, C5a (Sigma), IL-8, or MCP-1 (PeproTech) was added and fluorescence was recorded for an additional 50 s. Relative increase in fluorescence was expressed in comparison to uninhibited cells.
Spaan A.N., Vrieling M., Wallet P., Badiou C., Reyes-Robles T., Ohneck E.A., Benito Y., de Haas C.J., Day C.J., Jennings M.P., Lina G., Vandenesch F., van Kessel K.P., Torres V.J., van Strijp J.A, & Henry T. (2014). The Staphylococcal Toxins γ-Hemolysin AB and CB Differentially Target Phagocytes by Employing Specific Chemokine Receptors. Nature communications, 5, 5438.
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2

Calcium Mobilization in Leukocyte Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Activation of human neutrophils and monocytes was performed by real-time detection of calcium mobilization using flow cytometry18 (link). Human monocytes were labeled with an anti-CD14 monoclonal antibody (BD). Cells, labeled with Fluo-3AM, were pre-incubated with HlgA or HlgC for 1 min at room temperature. Subsequently, baseline fluorescence was recorded in a flow cytometer. After 10 s of baseline recording, C5a (Sigma), IL-8, or MCP-1 (PeproTech) was added and fluorescence was recorded for an additional 50 s. Relative increase in fluorescence was expressed in comparison to uninhibited cells.
Spaan A.N., Vrieling M., Wallet P., Badiou C., Reyes-Robles T., Ohneck E.A., Benito Y., de Haas C.J., Day C.J., Jennings M.P., Lina G., Vandenesch F., van Kessel K.P., Torres V.J., van Strijp J.A, & Henry T. (2014). The Staphylococcal Toxins γ-Hemolysin AB and CB Differentially Target Phagocytes by Employing Specific Chemokine Receptors. Nature communications, 5, 5438.
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