Human thyroid specimens were purchased from US Biomax and were trichrome stained or stained for eIF4E. Antigen retrieval was carried out as previously described (31 (link), 32 (link)). Photographs for quantitative comparison were taken using FeinOptic microscope and Jenoptik ProgRes C5 camera. Cell lines (K1, MDA-T85, CD18 and Panc1) growing on glass coverslips were stained for phalloidin (Alexa Fluor 488) at a ratio of 1:500 and pictures were taken using FeinOptic microscope and Jenoptik ProgRes C5 camera.
Feinoptic microscope
Manufactured by Jenoptik
The FeinOptic microscope is a high-precision optical instrument designed for detailed analysis and observation. It features advanced optics and engineering to provide users with a clear and detailed view of microscopic samples. The core function of the FeinOptic microscope is to magnify and display small-scale objects or structures for detailed examination.
Automatically generated - may contain errors
3 protocols using feinoptic microscope
1
Thyroid Specimen Staining and Cell Imaging
2
Immunohistochemical Analysis of Human Thyroid and Pancreatic Cancer Tissues
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Human thyroid tissue microarrays (TMAs) were purchased from US Biomax (Rockville, MD, USA), while human PDAC TMAs were created by the Pathology Core Facility at Northwestern University from de-identified PDAC specimens. The TMAs were stained for BRD4 (Rabbit, Abcam, Cambridge, United Kingdom) and hnRNPA1 (Rabbit, Abcam). CD18 tumors were stained for Ki67 (Rabbit, Cell Signaling) and cleaved caspase 3 (Rabbit, Cell Signaling). Antigen retrieval was carried out as previously described [42 (link),43 (link)]. Photographs for quantitative comparison were taken using FeinOptic microscope and Jenoptik ProgRes C5 camera.
3
Quantifying Pancreatic Tissue Changes
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Pancreatic tissue specimens from Kras and Kras/Slug mice were stained for amylase, CK19, PCNA, p-MLC2(S19) and p-ERK1/2(T202/Y204). Antigen retrieval was conducted as previously described10 (link)13 (link). Photographs for quantitative comparison were taken using FeinOptic microscope and Jenoptik ProgRes C5 camera. Number of Kras and Kras/Slug mice with no staining (0), less than 5% (1+), 5% to 25% (2+), or more than 25% (3+) of the pancreas with CK19 staining was determined. Relative number of p-ERK1/2(+) and PCNA(+) nuclei in Kras and Kras/Slug mice were quantified.
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