Pge2 elisa kit

We measured the level of PGE2 in a media of MSC cultures following treatment with H₂O₂ by a PGE2 Elisa kit (Elabscience, TX, USA) according to the manufacturer’s instructions.

RAW 264.7 and HaCaT cells (5 × 10⁶ cells/mL) were seeded in 6-well plates and cultured for 24 h. After treatment with CLE at different concentrations (25, 50, and 100 μg/mL) for 4 h, the cells were stimulated with LPS (200 ng/mL) or TNF-α/IFN-γ (10 ng/mL) for 24 h. DEX (5 µg/mL) was used as a positive control. Then, the culture supernatants were collected. The concentration of PGE2 in the supernatants of RAW 264.7 cells was detected using a PGE2 ELISA kit (Cat# E-EL-0034, Elabscience, Houston, TX, USA) according to the manufacturer’s instructions. HaCaT cells’ supernatants were analyzed using ELISA kits for TARC (EHCCL17, Invitrogen, Waltham, MA, USA), RANTES (EHRNTS, Invitrogen), Human IL-8 (BMS204-3, Invitrogen), Human Il-1β (RAB0273, Sigma Aldrich), and MCP-1 (BMS281, Invitrogen) according to the manufacturer’s instructions.

After mechanical stimulation, the levels of PGE2 hormone and IGF-1 protein in the culture supernatant of MLO-Y4 cells were assayed separately using a PGE2 ELISA kit and an IGF-1 ELISA kit (Elabscience Biotechnology Co., Ltd., Wuhan China) according to the manufacturers, instructions.