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2 protocols using oxacillin sodium salt

1

Antimicrobial and Proton Uncoupler Evaluation

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The berberine chloride hydrate (catalog number: B0450), ciprofloxacin hydrochloride monohydrate (catalog number: C2227), and tobramycin (catalog number: T2503) were purchased from the Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Oxacillin sodium salt (catalog number: sc-224180B) was purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Carbonyl cyanide 3-chlorophenylhydrazone (CCCP, catalog number: B34950) was purchased from Invitrogen (ThermoFisher Scientific Korea Ltd., Seoul, Republic of Korea). Ethidium bromide (catalog number: L07482) was purchased from Alfa Aesar (ThermoFisher Scientific Korea Ltd.). The berberine chloride hydrate was dissolved in DMSO (catalog number: 000D0458, Samchun Chemicals Co., Ltd.), and the ciprofloxacin hydrochloride monohydrate, EtBr, Oxacillin sodium salt, and tobramycin were dissolved in distilled water.
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2

Lipid Membrane Fluidity Analysis

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Oxacillin sodium salt (catalog number: sc-224180B, Santa Cruz Biotechnology Inc., Dallas, TX, USA) was dissolved in distilled water. Ricinoleic acid (catalog number: R7257, Sigma-Aldrich Inc., Seoul, Republic of Korea), palmitic acid (catalog number: 129702500, Acros Organics, ThermoFisher Scientific Korea Ltd., Seoul, Republic of Korea), stearic acid (catalog number: S0163, Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), linoleic acid (catalog number: L07949, ThermoFisher Scientific Korea), and oleic acid (catalog number: O0180, Tokyo Chemical Industry) were dissolved in ethanol (catalog number: 000E0243, Samchun Chemicals). Laurdan (6-Dodecanoyl-2-Dimethylaminonaphthalene, catalog number: D250) was purchased from Invitrogen (ThermoFisher Scientific Korea). Phosphate-buffered saline (PBS, catalog number: P5493-1L) was purchased from Sigma-Aldrich Inc.
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