Apc anti human cd336 nkp44

Manufactured by BioLegend

Sourced in United States

APC anti-human CD336 (NKp44) is a laboratory reagent used to identify and analyze natural killer (NK) cells that express the NKp44 activation receptor. This antibody is conjugated with the fluorescent dye Allophycocyanin (APC), allowing for flow cytometric detection and quantification of NKp44-positive cells.

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Lab products found in correlation

Fixable viability dye efluor 506, by Thermo Fisher Scientific (1 mentions) Cytoflex, by Beckman Coulter (1 mentions) Fitc anti human cd14, by BioLegend (1 mentions) Fitc anti human cd34, by BioLegend (1 mentions) Alexa fluor 700 anti human cd3, by BioLegend (1 mentions) Fitc anti human cd123, by BioLegend (1 mentions) Fitc anti human tcrαβ, by BioLegend (1 mentions) Fitc anti human tcrγδ, by BioLegend (1 mentions) Alexa fluor 488 anti human cd19, by BioLegend (1 mentions) Fitc anti human fcεriα, by BioLegend (1 mentions)

Spelling variants of this product

NKp44 (6 citations) NKp44-APC (5 citations) Anti-NKp44 (4 citations)

2 protocols using apc anti human cd336 nkp44

Multiparametric Flow Cytometry Profiling

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Flow cytometric analysis was performed using CytoFLEX (Beckman Coulter Life Sciences, Brea, CA, USA). The FlowJo software (BD Biosciences, San Jose, CA, USA) was used for cytometric data analysis. The antibodies used were as follows: PE/Cyanine7 anti-human CD337 (NKp30) (325214; BioLegend), APC anti-human CD336 (NKp44) (325110; BioLegend), PE/Dazzle 594 anti-human CD335 (NKp46) (331930; BioLegend), Brilliant Violet 421 anti-human CD226 (DNAM-1) (338332; BioLegend), Human NKG2C/CD159c Alexa Fluor 700 (FAB138N-100; R&D Systems, Minneapolis, MN, USA), APC/Cyanine7 anti-human CD314 (NKG2D) (320824; BioLegend), PE anti-human CD56 (NCAM) (318306; BioLegend), FITC anti-human CD3 (UCHT1) (11-0038-42; eBioscience, San Diego, CA, USA), APC anti-human CD181 (CXCR1) (320612; BioLegend) and APC anti-human CD182 (CXCR2) (320710; BioLegend). Dead cells were stained with the Fixable Viability Dye eFluor 506 (65-0866-14; eBioscience).

Yoon J.H., Yoon H.N., Kang H.J., Yoo H., Choi M.J., Chung J.Y., Seo M., Kim M., Lim S.O., Kim Y.J., Lee J.K, & Jang M. (2024). Empowering pancreatic tumor homing with augmented anti-tumor potency of CXCR2-tethered CAR-NK cells. Molecular Therapy Oncology, 32(1), 200777.

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Multiparametric Flow Cytometry Panel

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Alexa Fluor® 700 anti-human CD3, (clone: HIT3a), FITC-anti human TCRαβ (clone: IP26), FITC-anti-human TCRγδ (clone: B1), APC/Cy7 Anti-Human CD127 (I-7Ra), (clone: A019D5), PE anti-human CD161, (clone: HP-3G10), Brilliant Violet 421TM anti-human CD117 (c-kit), (clone 104D2), PE/Cy7 anti-human CD294 (CRTH2), (clone: BM16), APC anti-human CD336 (NKp44), (clone: 325110), Alexa Fluor® 488 anti-human CD19, (clone: HIB19), FITC anti-human CD94, (clone:DX22), FITC anti-human CD1a, (clone: HI149), FITC anti-human CD11c, (clone: 3.9), FITC anti-human CD123, (clone: 6H6), anti-human CD303 (BDCA-2), (clone:201A), FITC anti-human CD14, (clone: 63D3), FITC anti-human FcεRIα, (clone: NP4D6), FITC anti-human CD34,(clone: 561), APC/Cy7 anti-human IFN-γ, (clone: 4S.B3) all from BioLegend. Anti-Human CD363 (S1PR1) eFluor® 660, (clone: SW4GYPP, ThermoFisher), Mouse IgG1 K Isotype Control eFluor® 660, (clone: P3.6.2.8.1, ThermoFisher). Anti-mouse CD3) APC, (clone: 17A2), anti-mouse NK1.1 Alexa Fluor® 647, (clone: PK136), anti-mouse B220 APC/Cy7 or FITC, (clone:RA3-6B2), anti-mouse CD45-Percp Cy5.5, (clone:30-F11), anti-mouse CD90.2 PE Cy7, (clone:30-H12), anti-mouse CD11b APC, (clone:M1/70), anti-mouse GATA3 PE (Clone: 16E10A23), anti-mouse Rorγt (Clone: 2F7-2).

Eken A., Yetkin M.F., Vural A., Okus F.Z., Erdem S., Azizoglu Z.B., Haliloglu Y., Cakir M., Turkoglu E.M., Kilic O., Kara I., Dönmez Altuntaş H., Oukka M., Kutuk M.S., Mirza M, & Canatan H. (2019). Fingolimod Alters Tissue Distribution and Cytokine Production of Human and Murine Innate Lymphoid Cells. Frontiers in Immunology, 10, 217.

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