Apc cyanine7 conjugated anti cd45

Manufactured by BioLegend

Sourced in United States

The APC/cyanine7-conjugated anti-CD45 is a fluorescent-labeled antibody that binds to the CD45 cell surface antigen. CD45 is a receptor-linked protein tyrosine phosphatase expressed on the surface of most hematopoietic cells. This product can be used in flow cytometry applications to identify and characterize cell populations expressing CD45.

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Lab products found in correlation

Fitc conjugated anti cd3, by BioLegend (1 mentions) 7 aad viability staining, by BioLegend (1 mentions) Pe conjugated anti cd8a, by BioLegend (1 mentions) Aurora cs, by Cytek (1 mentions) Smartstrainer, by Miltenyi Biotec (1 mentions) Gentlemacs, by Miltenyi Biotec (1 mentions) Tumor dissociation kit, by Miltenyi Biotec (1 mentions) Anti mouse cd16 cd32 mouse fc block, by BD (1 mentions) Apc conjugated anti cd11b, by BioLegend (1 mentions) Ultracomp ebeads compensation beads, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti nk1, by BD (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions)

2 protocols using apc cyanine7 conjugated anti cd45

Profiling Tumor-Infiltrating T Cells

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To analyze the population
of T cells within the TME, cells were collected from the tumors on
day 10 following various treatments. This was achieved using a tumor
dissociation kit (Miltenyi Biotec, North Rhine-Westphalia, Germany)
along with a dissociator (GentleMACS, Miltenyi Biotec) to obtain single-cell
suspensions, which were then filtered through a 70 μm strainer
(Smartstrainer, Miltenyi Biotec) to eliminate larger debris. Thereafter,
the resulting single-cell suspensions underwent a blocking step with
antimouse CD16/32 antibodies (101302, 1:25, BioLegend) and were subsequently
stained with the following antibodies: APC/cyanine7- conjugated anti-CD45
(103116, 1:40, BioLegend), FITC-conjugated anti-CD3 (100204, 1:25,
BioLegend), PE-conjugated anti-CD8a (100708, 1:100, BioLegend), and
APC-conjugated anti-CD4 (100412, 1:100, BioLegend) antibodies. The
antibody staining process occurred over a period of 30 min at 4 °C,
followed by 7-AAD viability staining (420404, BioLegend). The stained
cells were then analyzed using a cell sorter (Aurora CS, Cytek Biosciences,
Fremont, CA, USA), and the data were analyzed using FlowJo Software
(Treestar, Ashland, OR, USA).

Ngo T.L., Wang K.L., Pan W.Y., Ruan T, & Lin Y.J. (2024). Immunomodulatory Prodrug Micelles Imitate Mild Heat Effects to Reshape Tumor Microenvironment for Enhanced Cancer Immunotherapy. ACS Nano, 18(7), 5632-5646.

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Comprehensive Lung Cell Immunophenotyping

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Single-lung-cell suspensions were blocked using anti-mouse CD16/CD32 (mouse BD Fc block, BD Biosciences, New Jersey, USA) at room temperature for 15 min prior to staining. Surface antigens were stained with the indicated conjugated antibodies at room temperature for 15 min. The following antibodies were used: APC-conjugated anti-CD4 (Thermo Fisher Scientific), PerCP-eFluor 710-conjugated anti-CD3e (Thermo Fisher Scientific), PE-Cyanine7-conjugated anti-CD45R (Thermo Fisher Scientific), APC/Cyanine7-conjugated anti-CD45 (BioLegend, San Diego, CA, USA), PE-conjugated anti-Siglec-F (Thermo Fisher Scientific), PerCD-eFluor710-conjugated anti-Ly6G, Alexa Fluor 700-conjugated anti-MHC Class II (I-A/I-E) (Thermo Fisher Scientific), eFluor 450-conjugated anti-F4/80 (Thermo Fisher Scientific), APC-conjugated anti-CD11b (BioLegend), FITC-conjugated anti-NK1.1 (BD Biosciences), PE-conjugated anti-CD49b (BioLegend), and PerCP-e710-conjugated anti-CD3e (BD Biosciences). For all experiments, cells were analyzed using a Gallios flow cytometer (Beckman Coulter). Panel setup and fluorescence compensation were performed using UltraCompeBeads™ Compensation Beads (Invitrogen). All analyses were performed using FlowJo software (Becton, Dickinson and Company, New Jersey, USA).

Kim C.U., Jeong Y.J., Lee P., Lee M.S., Park J.H., Kim Y.S, & Kim D.J. (2022). Extracellular nucleoprotein exacerbates influenza virus pathogenesis by activating Toll-like receptor 4 and the NLRP3 inflammasome. Cellular and Molecular Immunology, 19(6), 715-725.

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