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Fialuridine

Manufactured by Merck Group
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Fialuridine is a laboratory reagent manufactured by Merck Group. It is a nucleoside analog that can be used in various biochemical and molecular biology applications. The core function of Fialuridine is to serve as a research tool for studying cellular processes and biological phenomena.

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Lab products found in correlation

Tolvaptan, by Merck Group (1 mentions)

2 protocols using fialuridine

1

hiPSCs PB Cassette Removal

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To remove the PB cassette, corrected hiPSCs were transfected with PB Excision-Only Transposase vector (System Biosciences). Seventy-two hours after transfection, 0.2 μM fialuridine (Sigma) was used to eliminate PB cassette-containing clones for 5 days. Resistant colonies were picked and expanded, and further screened by junction PCR.
Xu X., Tay Y., Sim B., Yoon S.I., Huang Y., Ooi J., Utami K.H., Ziaei A., Ng B., Radulescu C., Low D., Ng A.Y., Loh M., Venkatesh B., Ginhoux F., Augustine G.J, & Pouladi M.A. (2017). Reversal of Phenotypic Abnormalities by CRISPR/Cas9-Mediated Gene Correction in Huntington Disease Patient-Derived Induced Pluripotent Stem Cells. Stem Cell Reports, 8(3), 619-633.
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2

Hepatocyte Spheroid Drug Screening

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Drug treatments were performed in n=6 spheroids per time point from a
single male preparation (N=1, pooled hepatocytes from 3 mice). ATP and albumin
data were generated from at least n=3–6 spheroids per time point, drug
and concentration, except for a single concentration on day 14 for both
acetaminophen (APAP) and tolvaptan (for which the data was obtained from n=2
spheroids due to spheroid loss during repeated media changes over time). APAP,
fialuridine, and tolvaptan were purchased from Sigma (St. Louis, MO, USA) and
AMG-009 was purchased from MedKoo Biosciences (Morrisville, NC, USA). Stock
solutions of chemicals were prepared in DMSO at 500X final concentration and
diluted in culture media to create dosing solutions with DMSO at 0.2% v/v.
Dosing solutions of APAP were prepared directly in culture media. The highest
concentration for each drug was based on the maximum solubility limit determined
either experimentally or from manufacturer’s product sheet. As in
baseline cultures, media was changed and fresh compound added every 2–3
days for the length of drug exposure.
Nautiyal M., Qasem R.J., Fallon J.K., Wolf K.K., Liu J., Dixon D., Smith P.C, & Mosedale M. (2020). Characterization of Primary Mouse Hepatocyte Spheroids as a Model System to Support Investigations of Drug-Induced Liver Injury. Toxicology in vitro : an international journal published in association with BIBRA, 70, 105010.
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