Gentra puregene blood core kit b

DNA was isolated from the whole (EDTA) blood samples using the Gentra Puregene Blood Core Kit B (Qiagen, Venlo, Netherlands) using a modified protocol as described previously [7 (link)]. DNA was isolated from hair samples using the Quick-DNA Miniprep Plus Kit (Zymo Research, Irvine, CA, USA) according to the manufacturer’s protocol and from semen (straw) using the Gentra Puregene Blood Core Kit B (Qiagen, Venlo, Netherlands) following the modified protocol described previously [8 (link)]. DNA was isolated from blood cards, tissue sampling units (TSUs), and tissue using the Gentra Puregene Blood Core Kit B (Qiagen, Venlo, Netherlands) with modifications (Additional File 2). All DNA was quantified using an Epoch Microplate Reader (BioTek, Winooski, VT, USA) and stored at -20˚C.

As already described by Bianchessi [17 (link)], genomic DNA (gDNA) was isolated from blood samples in ethylenediamine tetraacetic acid (EDTA) using a Gentra Puregene^® Blood Core Kit B (Qiagen, Venlo, The Netherlands; Carlsband, CA, USA).
RNA samples were collected in Tempus^TM Blood RNA Tubes (Life Technologies, Carlsbad, CA, USA) and extracted with a Tempus^TM Spin RNA Isolation Kit within 5 days. DNase treatment with Absolute RNA Wash Solution was performed for all samples during the RNA extraction protocol. RNA samples were reverse-transcribed using 50 units of High-Capacity cDNA Reverse Transcription mix (Life Technologies) and 20 units of RNAse inhibitor (Ambion, Austin, TX, USA). β-2-Microglobulin amplification was used as a quality control for retro-transcription.

DNA was isolated from ethylenediamine tetraacetic acid (EDTA) blood samples using a Gentra^® Puregene^® Blood Core Kit B (Qiagen, Venlo, Netherlands Carlsband, California, USA). RNA samples were collected in Tempus Blood RNA Tubes (Life Technologies, Carlsbad, CA) and extracted with a Tempus^™ Spin RNA Isolation Kit within 4 days. DNase treatment with Absolute RNA Wash Solution was performed for all samples during the RNA extraction protocol. RNA samples (1 μg) were reverse‐transcribed using 50 units of High‐Capacity cDNA Reverse Transcription mix (Life Technologies) and 20 units of RNAse inhibitor (Ambion^®, Austin, TX). Beta‐2‐Microglobulin amplification was used as a quality control for retro‐transcription.