Anti ter119 clone ter 119

Manufactured by BD

Anti-TER119 (clone TER-119) is a monoclonal antibody that binds to the TER-119 antigen expressed on erythroid cells. The antibody is used for the identification and enumeration of erythroid cells in flow cytometry and other applications.

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Lab products found in correlation

Anti cd11b clone m1 70, by BD (2 mentions) B cell isolation kit, by Miltenyi Biotec (2 mentions) Propidium iodide, by Merck Group (2 mentions) Anti cd4 clone gk1, by Thermo Fisher Scientific (2 mentions) Anti pgk clone 22c5d8, by Thermo Fisher Scientific (2 mentions) Anti mouse human actin clone ab 5, by BD (2 mentions) Horseradish peroxidase conjugated anti mouse ig, by GE Healthcare (2 mentions) Anti tcrβ clone h57, by BD (2 mentions) Anti cd19 clone 1d4, by BD (2 mentions) Allophycocyanin conjugated streptavidin, by Thermo Fisher Scientific (2 mentions) Phycoerythrin conjugated streptavidin, by Southern Biotech (2 mentions) Horseradish peroxidase conjugated goat anti rabbit ig, by Southern Biotech (2 mentions) Anti tcrγδ clone gl3, by BD (1 mentions) Pe cy5 anti mouse ly 6a e sca 1 clone d7, by Thermo Fisher Scientific (1 mentions) Anti cd34 clone ram34, by BD (1 mentions) Anti biotin microbead, by Miltenyi Biotec (1 mentions) Anti nk1.1 clone pk136, by BD (1 mentions) Anti cd19 clone 1d3, by Thermo Fisher Scientific (1 mentions) Magnetic activated cell sorting technology, by Miltenyi Biotec (1 mentions) Anti gr 1 clone rb6 8c5, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ter119 (80 citations) Anti-Ter119 (27 citations) TER-119 (clone Ter119, (7 citations) Anti-Ter119 (TER119; (5 citations)

5 protocols using anti ter119 clone ter 119

Multiparametric Immunophenotyping of Murine Stem Cells

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BMNC staining was performed in medium containing 2 % fetal bovine serum (FBS). All monoclonal antibodies (mAbs) were added at saturating concentrations and the cells were incubated for 30 min on ice, washed twice, resuspended in staining solution at a concentration of 5 × 10⁶ cells/ml, and subjected to analysis using an LSR II (Becton Dickinson, Mountainview, CA). The following anti-mouse Abs were used to detect fluorescein isothiocyanate (FITC)-anti-CD117 (c-Kit) (clone 2B8; BioLegend, San Diego, CA) and Phycoerythrin (PE)-Cy5 anti-mouse Ly-6A/E (Sca-1) (clone D7; eBioscience™, San Diego, CA). All anti-mouse lineage markers (Lin) were conjugated by PE and purchased from BD Biosciences: anti-CD45R/B220 (clone RA3-6B2); anti-Gr-1 (clone RB6-8C5); anti-T-cell receptor β (TCRβ; clone H57-597); anti-TCRγδ (clone GL3); anti-CD11b (clone M1/70); anti-Ter-119 (clone TER-119); and anti-CD34 (clone RAM34) as described [8 –10 (link)].

Wysoczynski M., Ratajczak J., Pedziwiatr D., Rokosh G., Bolli R, & Ratajczak M.Z. (2014). Identification of Heme Oxygenase 1 (HO-1) as a Novel Negative Regulator of Mobilization of Hematopoietic Stem/Progenitor Cells. Stem Cell Reviews, 11(1), 110-118.

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Antibodies and Biomolecules for Cellular Analysis

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Anti-PGK (clone 22C5D8, Invitrogen), anti-mouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma gondii actin^{39 (link)}, horseradish peroxidase-conjugated goat anti-rabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated anti-mouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19 (clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen), allophycocyanin-conjugated streptavidin (ebiosciences, cat. number 17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech, cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the experiments described here we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues, and with the following mutations: E105K/E108A^{40 (link)} and P94R/D160N/D165A/K190E/K196T^{24 (link)}: Sortase A was produced and purified as described elsewhere^{20 (link)}. Enhancer^{33 (link)} and VHH7^{34 (link)} were produced and purified as described elsewhere^{34 (link)}. Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG) was produced by the MIT biopolymer facility through standard solid phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere^{20 (link)}.

Swee L.K., Lourido S., Bell G.W., Ingram J.R, & Ploegh H.L. (2014). One-Step Enzymatic Modification of the Cell Surface Redirects Cellular Cytotoxicity and Parasite Tropism. ACS Chemical Biology, 10(2), 460-465.

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Engineered Sortase A for Bioconjugation

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Anti-PGK (clone 22C5D8, Invitrogen),
antimouse/human actin (clone Ab-5, BD biosciences), anti-Toxoplasma
gondii actin,^{39 (link)} horseradish peroxidase-conjugated
goat antirabbit Ig (Southern Biotech, cat. 4041-05), horseradish peroxidase-conjugated
antimouse Ig (GE Healthcare, cat. NXA931), anti-TCRβ (clone
H57, BD Pharmingen), anti-CD4 (clone GK1.5, ebiosciences), anti-CD19
(clone 1D4, BD Pharmingen), anti-TER119 (clone TER-119, BD Pharmingen),
allophycocyanin-conjugated streptavidin (ebiosciences, cat. number
17-4317), phycoerythrin-conjugated streptavidin (Southern Biotech,
cat. number 7100-09S). Propidium iodide (Sigma-Aldrich, cat. number
P4864). B cell isolation kit (Miltenyi, cat. 130-090-862). For the
experiments described here, we used a modified form of sortase A from Staphylococcus aureus that lacks the first 59 residues,
and with the following mutations: E105 K/E108A^{40 (link)} and P94R/D160N/D165A/K190E/K196T:^{24 (link)} Sortase A was produced and purified as described elsewhere.^{20 (link)} Enhancer^{33 (link)} and VHH7^{34 (link)} were produced and purified as described elsewhere.^{34 (link)} Biotin-LPETG (Biotin- aminohexanoic acid-LPETGG)
was produced by the MIT biopolymer facility through standard solid
phase peptide synthesis. TAMRA-LPETG was produced as described elsewhere.^{20 (link)}

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Xenograft Model of Sickle Cell Disease

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NOD/SCID mice received intraperitoneal injections of 5 × 10⁹ G6PDd‐huRBCs daily for 14 consecutive days as previously described.²⁹ Post‐engraftment, tail‐vein blood was collected for flow cytometry and stained for mouse red blood cells (muRBC) with anti‐TER‐119 (clone TER‐119; BD Pharmingen, Franklin Lakes), huRBC with anti‐Glycophorin A (clone YTH89.1; Abcam, Cambridge, MA), and muRetics with anti‐CD71 (clone R172717; Invitrogen, Waltham, MA). Inclusion criteria for all experiments was >60% circulating peripheral huRBCs. Mice were subsequently randomized into the experimental groups.

Flaherty S., Strauch P., Maktabi M., Pybus B.S., Reichard G., Walker L.A, & Rochford R. (2022). Mechanisms of 8‐aminoquinoline induced haemolytic toxicity in a G6PDd humanized mouse model. Journal of Cellular and Molecular Medicine, 26(13), 3675-3686.

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Adoptive T Cell Transfer to RR22 Mice

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Cells from the spleen and lymph nodes of Actb^ECFP reporter or Ccr9^−/− mice were isolated by being passed through a steel wire mesh, filtered on a 40-μm cell strainer and after red blood cells lysis using ACK buffer. To enrich for T cells, single-cell suspensions were first incubated with biotin-conjugated anti-B220 (clone RA3-6B2; eBioscience), anti-CD19 (clone 1D3; eBioscience), anti-CD11b (clone M1/70; BD Biosciences), anti-CD11c (clone N418; eBioscience), anti-Gr1 (clone RB6-8C5; eBioscience); anti-NK1.1 (clone PK136; BD Biosciences) and anti-Ter119 (clone TER-119; BD Biosciences) antibodies followed by antibiotin microbeads (Miltenyi Biotec) and negatively selected by magnetic cell separation with magnetic-activated cell sorting technology (Miltenyi Biotec); 5 × 10⁶ enriched T cells were intravenously transferred to Rag2^−/−Rorc^GFPIl22^TdT (RR22) mice; recipient mice were analyzed at day 14 post-transfer.

Jarade A., Garcia Z., Marie S., Demera A., Prinz I., Bousso P., Di Santo J.P, & Serafini N. (2022). Inflammation triggers ILC3 patrolling of the intestinal barrier. Nature Immunology, 23(9), 1317-1323.

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