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β tubulin primary antibody

Manufactured by Abcam
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The β-tubulin primary antibody is a protein that recognizes and binds to the beta subunit of the tubulin protein. Tubulin is a key structural component of microtubules, which are important for various cellular processes such as cell division, intracellular transport, and cell motility.

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Lab products found in correlation

Alexa fluor 488 conjugated affinipure donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

β-tubulin antibody β-tubulin

3 protocols using β tubulin primary antibody

1

Western Blot Antibody Validation

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Primary antibodies targeting p-AKT (S473), AKT, p-EGFR (Y1068), EGFR, p-S6, and S6 were purchased from Cell Signaling Technology (Boston, MA, USA). β-tubulin primary antibody was purchased from Abcam (Cambridge, MA, USA). Secondary antibodies were purchased from Bio-Rad (Hercules, CA, USA).
Li H., Peyser N.D., Zeng Y., Ha P.K., Johnson D.E, & Grandis J.R. (2022). NSAIDs Overcome PIK3CA Mutation-Mediated Resistance to EGFR Inhibition in Head and Neck Cancer Preclinical Models. Cancers, 14(3), 506.
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2

Quantitative Western Blot Analysis

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Primary antibodies for pSTAT3 (Y705) and STAT3 were obtained from Cell Signaling Technology (Danvers, MA). β-tubulin primary antibody was purchased from Abcam (Cambridge, MA). Secondary antibodies were purchased from BioRad (Hercules, CA). Blots were quantitated by densitometry using ImageJ software.
Peyser N.D., Du Y., Li H., Lui V., Xiao X., Chan T.A, & Grandis J.R. (2015). Loss-of-Function PTPRD Mutations Lead to Increased STAT3 Activation and Sensitivity to STAT3 Inhibition in Head and Neck Cancer. PLoS ONE, 10(8), e0135750.
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3

Rhodamine Nanoparticle Localization in bEnd.3 Cells

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Rhodamine nanoparticle localization was studied qualitatively by with immunohistochemistry. bEnd.3 cells were plated
on coverslips and incubated with rhodamine labelled nanoparticles for a period of six hours. Upon completion of the incubation
period, the cells were washed three times with PBS, fixed with 4% paraformaldehyde for 15 minutes, and permeabilized with 0.1%
Triton-X100 for 10 minutes. Upon permeabilization, the cells were blocked with 10% v/v goat serum in 5% w/v bovine serum
albumin (BSA). Subsequently, the cells were incubated in β-tubulin primary antibody (Abcam ab15568) overnight at
4°C. Alexa Fluor 488-conjugated AffiniPure donkey anti-rabbit IgG (Jackson Immuno Research Laboratories, West Grove,
PA) was used as the secondary antibody in 5% BSA for two hours at room temperature. Finally, the cell nuclei were stained with
DAPI (Thermo Scientific) for ten minutes, prior to mounting the coverslips on to slides. Cells were washed thrice with PBS
between each step. The cells were imaged using Nikon A1R/SIM confocal and super resolution imaging microscope (Nikon, Tokyo,
Japan).
Saralkar P., Arsiwala T, & Geldenhuys W.J. (2020). Nanoparticle formulation and in vitro efficacy testing of the mitoNEET ligand NL-1 for drug delivery in a brain endothelial model of ischemic reperfusion-injury. International journal of pharmaceutics, 578, 119090.
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