Log In Sign up
The largest database of trusted experimental protocols

Histogrip

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

Histogrip is a laboratory equipment product designed to facilitate the handling and positioning of tissue samples during histological procedures. It provides a secure grip on the samples, allowing for precise and controlled manipulation during the preparation and analysis stages.

Automatically generated - may contain errors

Lab products found in correlation

Lsm image browser version 4, by Zeiss (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Donkey serum, by Merck Group (1 mentions) Carl lsm 780, by Zeiss (1 mentions) Tp1020 tissue processor, by Leica (1 mentions) H1500, by Zeiss (1 mentions)

4 protocols using histogrip

1

Immunofluorescence Imaging of TF-1 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
TF-1 cells were collected on histogrip (Invitrogen, Frederick, MD, USA) coated slides by cytospin (Shandon Cytospin 3, Pittsburgh, PA, USA), were fixed with 4% paraformaldehyde for 15 min at room temperature (RT) and permeabilized in 0.1% Triton X-100 in PBS for 10 min at RT, followed by blocking with 10% donkey serum (Sigma-Aldrich, St. Louis, MO, USA) in PBS for 1 h at RT. Primary antibodies were applied at the manufacturer's recommended concentrations, followed by overnight incubation in a humidified chamber at 4 °C. Fluorophore-conjugated secondary antibodies were applied at the dilution of 1:500 and incubated for 2 h at RT. The cells were mounted with Prolong Gold antifade reagent with DAPI (Invitrogen, Eugene, OR, USA) for nuclear counterstaining. Images were captured at a resolution of 2048 × 2048 using a Carl Zeiss LSM 780 (Carl Zeiss, Jena, Germany) confocal microscope with a × 40 Plan objective lens. The LSM Image Browser version 4.2.0.121 (Carl Zeiss MicroImaging, Jena, Germany) was used to analyze the microscopic slides.
Ren Z., Aerts J.L., Vandenplas H., Wang J.A., Gorbenko O., Chen J.P., Giron P., Heirman C., Goyvaerts C., Zacksenhaus E., Minden M.D., Stambolic V., Breckpot K, & De Grève J. (2016). Phosphorylated STAT5 regulates p53 expression via BRCA1/BARD1-NPM1 and MDM2. Cell Death & Disease, 7(12), e2560-.
+ Open protocol
+ Expand
2

Histological Detection of TiLV in Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols
The preserved tissues were dehydrated by incubating in several increasing concentrations of ethanol (70-100%) and then transferred to xylene automatically by Leica TP1020 Tissue Processor (Leica Biosystems, US). The tissues were infiltrated and embedded in paraffin. Each paraffinembedded tissue was sequentially sectioned at 5 μm thickness into 5 consecutive sections and were mounted on HistoGrip (Invitrogen, US) coated glass slides. The purposed tissue sections included 2 slides for ISH with TiLV specific probes derived from TiLV genome segment 1 and segment 3 (see below), 2 slides for ISH with negative controls (unrelated probe and no probe), and one slide for hematoxylin and eosin (H&E) staining. To analyze the distribution of TiLV in the whole-brain, the samples were sectioned toward horizontal and parasagittal sides and the results were interpreted in parallel.
Dinh-Hung N., Sangpo P., Kruangkum T., Kayansamruaj P., Rung-Ruangkijkrai T., Senapin S., Rodkhum C, & Dong H.T. (2021). Dissecting the localization of Tilapia tilapinevirus in the brain of the experimentally infected Nile tilapia, Oreochromis niloticus (L.). Journal of fish diseases, 44(8).
+ Open protocol
+ Expand
3

Cryosectioning and Imaging of Bone Samples

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The right femur from each mouse was fixed in 10% neutral buffered formalin and cryosectioned longitudinally (undecalcified, 5 μm thickness) using Cryofilm IIC, as previously described [18 (link)]. Two RD exercise and one VEDD exercise samples were damaged during processing and were subsequently excluded from analysis. To detect Evans Blue staining, sections were imaged in PBS containing FM1-43 dye (used to label intracellular membranes and confirm the presence of an intracellular Evans Blue signal) on LSM780 multiphoton confocal microscope. Images were captured as a Z-stack (series of 20-30 images through section thickness) and processed into a maximum intensity projection. Five to ten images per bone were captured and analyzed in Bioquant Osteo software to quantify percentage of Evans Blue labelled osteocytes [18 (link)]. To validate Evans Blue staining, the left tibia from a subset of mice (n=4 to 7 mice per group) was decalcified, cryosectioned (7 μm) onto Histogrip (LifeTechnologies #008050) coated slides, and immunohistochemically stained to detect albumin as an endogenous PMD tracer, as we previously described [18 (link)]. Albumin was detected with a FITC-conjugated antibody (AIFAG3140, Accurate Chemical & Scientific Company, 1:100 dilution), and sections were mounted with DAPI (Vectashield H1500) and imaged with a confocal microscope (Zeiss).
Hagan M.L., Bahraini A., Pierce J.L., Bass S.M., Yu K., Elsayed R., Elsalanty M., Johnson M.H., McNeil A., McNeil P.L, & McGee-Lawrence M.E. (2018). Inhibition of osteocyte membrane repair activity via dietary Vitamin E deprivation impairs osteocyte survival. Calcified tissue international, 104(2), 224-234.
+ Open protocol
+ Expand
4

Quantitative Multiplex Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
From TMA blocks, 5-μm sections were cut, placed on Histogrip (Life Technologies)-coated slides, and processed as described previously (Supplementary Materials). Briefly, after deparaffinisation, antigen retrieval was performed in 0.05% citraconic anhydride solution for 45 min at 95 °C using a Lab Vision PT module (Thermo Scientific, Waltham, MA, USA). Staining was performed either manually or in automated fashion with an Autostainer 360 or 720 (Thermo Scientific).
The quantitative multiplex immunofluorescence (QMIF) staining procedure that combined two anti-biomarker antibodies with region-of-interest markers was performed as previously described (see Supplementary Materials and Methods).
Shipitsin M., Small C., Choudhury S., Giladi E., Friedlander S., Nardone J., Hussain S., Hurley A.D., Ernst C., Huang Y.E., Chang H., Nifong T.P., Rimm D.L., Dunyak J., Loda M., Berman D.M, & Blume-Jensen P. (2014). Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling error. British Journal of Cancer, 111(6), 1201-1212.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!