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Anti mmp 3 antibody

Manufactured by R&D Systems
Sourced in United States

The Anti MMP-3 antibody is a laboratory reagent designed for research purposes. It is a specific antibody that can be used to detect and measure the presence of the MMP-3 protein in biological samples.

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2 protocols using anti mmp 3 antibody

1

Antibody Sources and Inhibitors Used

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The mouse monoclonal anti MMP-3 antibody was from R&D Systems (Minneapolis, MN, USA), the rabbit polyclonal antibody to IKKɛ from Cell Signaling Technology (Danvers, MA, USA), the mouse monoclonal antibody to β-actin from Sigma Aldrich (St Louis, MO, USA), antibodies to Akt, p-Akt, ERK and p-ERK from Cell Signaling Technology and the polyclonal rabbit antibodies to USF2 and p65 from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All restriction enzymes were obtained from New England Biolabs (Ipswich, MA, USA). PMA was from Sigma-Aldrich Canada (Oakville, ON, Canada). The inhibitors Ro31–8220, Go6976 and JSH-23 were from Calbiochem (Gibbstown, NJ, USA) and PD98059 and LY294002 were from Cell Signaling Technology.
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2

Chondrocyte Culture and Inflammatory Markers

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Chondrocyte growth media were purchased from Cell Signaling Technology (411–500, Beverly, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotics (penicillin/streptomycin) were purchased from Welgene (Kyungsan, Korea). Anti-human MMP-1 (SC 58377) and anti-MMP-13 (SC 515284), anti-INOS (SC 650), anti-COX-2 (SC 1746), anti-IL-1β (SC 7884), and anti-GAPDH (SC 166574) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-MMP-3 antibody was purchased from R&D Systems (Minneapolis, MN, USA). The primers used in PCR analyses were obtained from Bioneer (Daejeon, Korea).
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