Egg yolk lecithin

Egg yolk lecithin was provided by Lipoid Gmbh (Ludwigshafen, Germany). Cholesterol was purchased from Beijing Bailingwei Technology Co., Ltd. (Beijing, China). Ethylenediamine and ammonium bicarbonate (NH₄HCO₃) were purchased from Damao chemical reagent factory (Tianjin, China). Anhydrous sodium acetate and FeCl₃·6H₂O were obtained from Tianjin Hengxing Chemical Preparation CO., Ltd. (Tianjin, China). Ethylene glycol was provided by Tianjin Fuyu Fine Chemical CO., Ltd. (Tianjin, China). Fructus Toosendan and Rhizoma Corydalis were provided by GuoDa Pharmacy (Shenyang, China). COR, TSN, and PRO standards were obtained from Chendu Pufei De Biotech CO., Ltd. (Sichuan, China). The reference standards of THB, THC, DHC, and JAT were purchased from Chendu Herbpurify CO., Ltd. (Sichuan, China). THP, ALL, COP, BER, and palmatine chloride standards were provided by Chengdu MUST Bio-tech Co., Ltd. (Sichuan, China). The reference standard of RUT was provided by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). HPLC-grade acetonitrile was provided by Concord Technology CO., Ltd. (Tianjin, China). Formic acid (HPLC grade) was purchased from DIKMA Technologies, Inc. (Beijing, China). Deionized water used throughout the study was provided by Wahaha Corporation (Hangzhou, China).

A. satureioides was acquired from Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA) da Universidade Estadual de Campinas (São Paulo, Brazil), via a sample deposited in the herbarium with the number 308. Egg yolk lecithin and medium-chain triglycerides were purchased from Lipoid (Ludwigshafen, Germany), polysorbate 80 was acquired from Vetec (Rio de Janeiro, Brazil), and vitamin E was procured from Alpha Química (Cachoeirinha, Brazil). Ultra-fast liquid chromatography (UFLC) required the following reagents: methanol (J.T. Baker, Philipsburg, NJ, USA), acetonitrile (Tedia, Rio de Janeiro, Brazil), and phosphoric acid (Merck, Rio de Janeiro, Brazil). Carbopol^® Ultrez 20 was kindly donated by Lubrizol do Brasil Aditivos Ltd.a (São Paulo, Brazil). Albumin for enzyme-linked immunosorbent assay (ELISA) was purchased from Calbiochem (San Diego, CA, USA). Normal goat serum was obtained from R&D Systems (Minneapolis, MN, USA). Protease inhibitor cocktail was purchased from VWR Life Science (Radnor Township, PA, USA). Xylazine and ketamine were purchased from Ceva (Paulínea, Brazil). Ketoprofen was obtained from Merial (Paulínea, Brazil). Finally, thiopental was obtained from Cristália (Itapira, Brazil).