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Lsm710nlo confocal laser scanning microscope

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The LSM710NLO is a confocal laser scanning microscope manufactured by Zeiss. It is designed for high-resolution imaging of specimens, utilizing a non-linear optical (NLO) excitation technique. The microscope is capable of capturing detailed images by scanning the sample with a focused laser beam and collecting the emitted fluorescence or reflected light.

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Lab products found in correlation

Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Motorized stage, by MBF Biosciences (1 mentions) Dylight 488, by Vector Laboratories (1 mentions) Alexa 594, by Abcam (1 mentions) Ix2 ucb microscope, by Olympus (1 mentions) Em ccd camera, by Hamamatsu Photonics (1 mentions) Alexa 488, by Abcam (1 mentions) Dylight 594, by Vector Laboratories (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Zen 2009, by Zeiss (1 mentions) Avidin biotin blocking kit, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Carl Roth (1 mentions) Cellmask orange plasma membrane stain, by Thermo Fisher Scientific (1 mentions) Lysotracker green dnd 26, by Thermo Fisher Scientific (1 mentions) 8 well chamber slide, by Thermo Fisher Scientific (1 mentions) Plan neofluar 20x 0.50 ph2 objective, by Zeiss (1 mentions) Pluronic f 127, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions)

14 protocols using lsm710nlo confocal laser scanning microscope

1

Protein-Protein Interaction Visualization

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The full-length coding sequences without the stop codons of the selected Fd-interacting proteins and FdC1 were amplified and cloned into the N-termini of truncated eYFP fused vectors, pSPYNE-35S (prey proteins) and pSPYCE-35S (bait proteins), respectively (Hu et al., 2006 ). After transforming into the Agrobacterium tumefaciens strain GV3101, the agrobacteria were co-infiltrated into the epidermal cell layers of 30-day-old tobacco leaves via syringe injection (Kerppola, 2006 (link)). After incubation in darkness for 48 h, the transfected leaves were examined for fluorescence emission under a LSM 710 NLO Confocal Laser Scanning Microscope (Carl Zeiss, Germany).
Guan X., Chen S., Voon C.P., Wong K.B., Tikkanen M, & Lim B.L. (2018). FdC1 and Leaf-Type Ferredoxins Channel Electrons From Photosystem I to Different Downstream Electron Acceptors. Frontiers in Plant Science, 9, 410.
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2

Subcellular Localization of NAC62 and WRKY1

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Full-length CitNAC62 and full-length CitWRKY1 were cloned into either C-terminal or N-terminal fragments of yellow fluorescent protein (YFP) vectors (Sainsbury et al., 2009 (link)). Primers used are listed in Supplementary Table S4. All constructs were transiently expressed in tobacco leaves by Agrobacterium-mediated infiltration (GV3101) based on previous reports with some modifications (Li et al., 2016 (link)). The YFP fluorescence of tobacco leaves was imaged 3 d after infiltration using a Zeiss LSM710NLO confocal laser scanning microscope.
Li S.J., Yin X.R., Wang W.L., Liu X.F., Zhang B, & Chen K.S. (2017). Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3. Journal of Experimental Botany, 68(13), 3419-3426.
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3

Subcellular Localization of AhTPP Proteins

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The coding sequences of AhTPP1 and AhTPP5 were PCR amplified with cDNA as the template, and cloned into the pCambia1300-35S::GFP binary vector. Table S10 provides detailed information on gene access numbers and primer sequences used for cloning purposes. Upon confirming the generation of pCambia1300-35S:AhTPPs-GFP vectors through sequencing, they were subsequently introduced into tobacco protoplasts that expressed a red fluorescent nuclear marker (mcherry). The transformation process was carried out using the PEG-mediated method [54 (link)]. Following a 48h incubation period, the signals of green fluorescent protein (GFP), red fluorescent protein (RFP), and bright field were visualized using a Zeiss (Carl Zeiss AG, Oberkochen, Baden-Wurttemberg, Geramny) LSM710NLO confocal laser scanning microscope.
Liu Y., Wang X., Ouyang L., Yao R., Wang Z., Kang Y., Yan L., Chen Y., Huai D., Wang Q., Jiang H., Lei Y, & Liao B. (2024). Genome-Wide Analysis of Trehalose-6-Phosphate Phosphatase Gene Family and Their Expression Profiles in Response to Abiotic Stress in Groundnut. Plants, 13(8), 1056.
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4

Subcellular Localization of PpUGT85A2

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The recombined 35S-PpUGT85A2-GFP vectors were constructed using primers listed in Supplementary Table S5, and were transformed into A. tumefaciens strain GV3101::pSoup by the Gene Pluser Xcell Electroporation System (Bio-Rad) according to Li et al. (2017) (link). To identify the intracellular structure, the vector was infiltrated into transgenic tobacco (Nicotiana benthamiana) plants expressing a red fluorescent nuclear marker (Nucleus–RFP). After 3 d infiltration, the green fluorescent protein (GFP) fluorescence of tobacco leaves was imaged using a Zeiss LSM710NLO confocal laser scanning microscope. Three replications were performed to confirm subcellular localization.
Wu B., Cao X., Liu H., Zhu C., Klee H., Zhang B, & Chen K. (2018). UDP-glucosyltransferase PpUGT85A2 controls volatile glycosylation in peach. Journal of Experimental Botany, 70(3), 925-936.
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5

Multiparametric Immunofluorescence Assay

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Free-floating sections were blocked in 5% horse serum and incubated in 1% BSA with the following primary antibodies raised against: hα-synuclein (syn211; 1:3000), GFP (ab13970; 1:5000), choline acetyltransferase (ChAT) (AB144, Merck Millipore; 1:100), Syn-O2 (1:2000) or Syn-F1 (1:1000). The latter two antibodies, gifts from Dr. O. El-Agnaf, have been previously characterized [26 (link)]. Appropriate fluorophore-conjugated secondary antibodies (Dylight 488 and Dylight 594, from Vector Laboratories; Alexa 488 and Alexa 594 from Abcam) (1:400) were used for detection, and samples were mounted and coverslipped using Vectashield mounting medium (Vector Laboratories). Sequential scans were performed with 10x and 63x Plan-Apochromat objectives using either (i) a LSM710NLO confocal laser scanning microscope (Carl Zeiss) with tunable lasers set at 490 nm and 595 nm, or (ii) an IX2 UCB microscope (Olympus) with a DSU spinning disk unit (Olympus), a motorized stage (MBF Biosciences) and a EM-CCD camera (Hamamatsu). As negative controls, tissue sections were processed as described above with the only exception that the primary antibody (e.g., anti-ChAT) was omitted from the initial incubations (Supplementary Fig. 1).
Ulusoy A., Phillips R.J., Helwig M., Klinkenberg M., Powley T.L, & Di Monte D.A. (2016). Brain-to-stomach transfer of α-synuclein via vagal preganglionic projections. Acta neuropathologica, 133(3), 381-393.
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6

Yeast Complementation Assay for SWEET Proteins

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The yeast complementation vectors or empty vectors were transformed into the hexose-uptake deficient yeast mutant EYB4000 (Wieczorke et al., 1999 (link)). Then, transformed yeasts were screened in synthetic dropout (SD)-Ura media, supplemented with 2% maltose. For complementation growth assays, yeasts were grown overnight in liquid SD media to an optical density at 600 nm (OD600) of ~0.6, then OD600 was adjusted to ~0.3 with water. Five-microliter aliquots of serial dilutions were plated on SD media containing 2% maltose (as control) or 2% other hexoses. Growth photographs were taken after incubation at 30 °C for three days.
For subcellular localization of SWEET proteins in yeast cells, transformed yeasts cultured in SD media supplemented with 2% maltose were collected, washed three times with water, and then applied on microscope slide. Fluorescence signals were detected using a Zeiss LSM710NLO confocal laser-scanning microscope. Excitation/emission wavelength were 488 nm for GFP signal.
Yue W., Cai K., Xia X., Liu L, & Wang J. (2023). Genome-wide identification, expression pattern and genetic variation analysis of SWEET gene family in barley reveal the artificial selection of HvSWEET1a during domestication and improvement. Frontiers in Plant Science, 14, 1137434.
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7

Analyzing Pollen Tube Growth in Goniothalamus

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Possible intercarpellary growth of pollen tubes was investigated using Goniothalamus parallelivenius and G. tapisoides. Thirty flower buds were bagged prior to anthesis, and pollen from staminate‐phase flowers used for hand pollination of pistillate‐phase flowers borne on other individuals. The latter flowers were collected 1 d after pollination, since previous studies of Annona cherimola (which similarly exhibits 2 d anthesis) have demonstrated ovule fertilization within 24 h of pollination (Lora et al. 2011, 2010). The samples were fixed in formalin‐acetic acid‐alcohol (FAA) overnight and then transferred to 70% ethanol for long‐term storage. Carpels and part of the attached receptacle were excised and immersed in 5% KOH at ambient temperature until they became translucent. The specimens were then rinsed with distilled water and stained with aniline blue (0.1% in 0.03 mol/L K3PO4) overnight, with the carpels then squashed with glycerine under a cover slip (Kho and Baer 1968; Du and Wang 2012). The preparations were examined with a Carl Zeiss LSM 710 NLO confocal laser scanning microscope with green fluorescent protein (GFP) filter.
Lau J.Y., Pang C., Ramsden L, & Saunders R.M. (2017). Stigmatic exudate in the Annonaceae: Pollinator reward, pollen germination medium or extragynoecial compitum?. Journal of Integrative Plant Biology, 59(12), 881-894.
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8

Visualizing OsLEC1-GFP Localization in Rice Protoplasts

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Rice protoplasts were prepared and transformed as previously described (Bai et al., 2014 (link)). Approximately 8 μg of the expression vector 35S:OsLEC1-GFP was transferred into rice protoplasts, then, the protoplasts were cultured at room temperature in the dark for 16 h. Fluorescence signals were detected and photographed using an LSM710 NLO confocal laser scanning microscope (Zeiss, Mannheim, Germany). Excitation/emission wavelengths were 488 nm for GFP and 561/575–630 nm for mCherry. Fluorescence signals were analyzed using the Zen2009 (Carl Zeiss) software.
Guo F., Zhang P., Wu Y., Lian G., Yang Z., Liu W., Buerte B., Zhou C., Zhang W., Li D., Han N., Tong Z., Zhu M., Xu L., Chen M, & Bian H. (2022). Rice LEAFY COTYLEDON1 Hinders Embryo Greening During the Seed Development. Frontiers in Plant Science, 13, 887980.
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9

Imaging Phagocytosis of Polyphosphates

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BMDM (5 × 104/well) were seeded in 8-well chamber slides (Thermo Fisher Scientific) in RPMI 1640 medium without phenol red, supplemented with 100 U/ml penicillin-streptomycin (Thermo Fisher Scientific) and 0.1% (w/v) bovine serum albumin (Carl Roth) and allowed to adhere firmly overnight. The next day, BMDM were washed and maintained in HBSS for the staining procedure. After treating the surfaces with biotin/streptavidin blocking reagent (Avidin/Biotin Blocking Kit, Thermo Fisher), preformed complexes of 50 µM biotin-labeled L-polyphosphates and Streptavidin-PE or Streptavidin-FITC (BioLegend), generated by 30-min incubation of the reagents on ice, were added to the cells for 1 h at 37 °C. LysoTracker Green DND-26 (100 nM, Thermo Fisher Scientific) or CellMask Orange Plasma Membrane Stain (5 µg/ml, Thermo Fisher Scientific) were added gently together with the nuclear dye, Hoechst 33342 (Thermo Fisher Scientific), for another 30 min of incubation. The macrophages were washed thoroughly with HBSS to remove excess dyes and immediately imaged using a Zeiss LSM 710 NLO confocal laser scanning microscope (CLSM) with a 1.4 Oil DIC M27 ×63 plan-apochromat oil objective.
Roewe J., Stavrides G., Strueve M., Sharma A., Marini F., Mann A., Smith S.A., Kaya Z., Strobl B., Mueller M., Reinhardt C., Morrissey J.H, & Bosmann M. (2020). Bacterial polyphosphates interfere with the innate host defense to infection. Nature Communications, 11, 4035.
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10

Visualizing PpGST1 Subcellular Localization

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To examine the subcellular localization of PpGST1, PpGST1‐GFP was transiently expressed in N. benthamiana leaves by Agrobacterium infiltration (GV3101) with the method described in the dual‐luciferase assay above. Tonoplast marker and transgenic N. benthamiana plant with a red fluorescent nuclear marker (Nucleus‐RFP) were used to identify the intracellular structure. On the third day after infiltration, the GFP florescence of the transiently infected leaves was measured by a Zeiss LSM710NLO confocal laser scanning microscope.
Zhao Y., Dong W., Zhu Y., Allan A.C., Lin‐Wang K, & Xu C. (2019). PpGST1, an anthocyanin‐related glutathione S‐transferase gene, is essential for fruit coloration in peach. Plant Biotechnology Journal, 18(5), 1284-1295.
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