Uv vis 1601 spectrophotometer

10 mg of rutin reference with constant weight is weighed precisely and put into 50 mL volumetric flask with a small amount of 70 % ethanol and then diluted to the volume. It was shaken well and put aside. The exact amount is taken from solution 0.5, 1, 2, 3, 4 and 5 mL placed in 25 mL volumetric flasks, respectively, and added with distilled water (3 mL) and 5% NANO2 (1 mL). After standing for 6 minutes, it was combined with 10% AlCl3 solution (1mL), 4% NaOH (10 mL) and diluted with distilled water to the volume. The final concentrations were 4, 8, 16, 24, 32 and 40 µg/mL of rutin. After 15 min, the absorbance was measured at 500 nm, in a UV/Vis spectrophotometer. 70% ethanol was used as a blank.
Determination of total coumarin content: 1 mL of solution A was transferred to a 25 mL volumetric flask diluted with 70% ethanol to the volume. The absorbance solution was measured after 15 min at room temperature at 336 nm in UV/Vis spectrophotometer-1601 (Shimadzu, Japan) [8, 14] . 70% ethanol was used as a blank.

15 mg of gallic acid reference substance, with constant weight, is weighed precisely and put into 50 mL volumetric flask with a small amount of distilled water and then diluted to the volume. It was shaken well, put aside. The exact amount is taken from solution 1, 3, 5, 7 and 9 mL placed in 25 mL volumetric flasks, respectively, and diluted to the volume. The final concentrations were 12, 36, 60, 84 and 108 µg/mL of galllic acid. Then, a mixture was prepared with 0.5 mL of this each stock solution, 1mL Folin-Ciocalteu reagent, and 10 mL of water, and volumetrically diluted to 25 mL with 10.75% sodium carbonate (w/v). After 30 min, the absorbance was measured at 760 nm, using water as the compensation liquid and quartz cell (1 cm path length) in a UV/Vis spectrophotometer.
Determination of total flavonoid content: 3 mL of solution A was transferred to a 25 mL volumetric flask containing distilled water (6 mL) and 5% NANO2 (1 mL). After standing for 6 minutes, 10% AlCl3 solution (1mL) and 4% NaOH (10 mL) were added and then diluted with distilled water to the volume. The absorbance of the red colored solution was measured after 15 min at room temperature at 500 nm in UV/Vis spectrophotometer-1601 (Shimadzu, Japan) [1, (link)10] . 70% ethanol was used as a blank.

2 mg of isofraxedin reference substance with a constant weight is weighed precisely and put into 10 mL volumetric flask with small amount of 70% ethanol and then diluted to the Proceedings of the Mongolian Academy of Sciences PMAS volume. It was shaken well and kept aside. The exact amount is taken from solution 1.5, 2, 2.5, and 3 mL is placed in 10 mL volumetric flasks respectively and diluted to the volume. The final concentrations were 30, 40, 50 and 60 µg/mL of isofraxedin. After 15 min, the absorbance was measured at 336 nm, in a UV/Vis spectrophotometer. 70% ethanol was used as a blank.
Determination of total alkaloid content: 1 mL solution A was transferred to a 25 mL volumetric flask diluted with 70% ethanol to the volume. The absorbance solution was measured after 15 min at room temperature at 343 nm in UV/Vis spectrophotometer-1601 (Shimadzu, Japan) [5, 7, 12] . 70% ethanol was used as a blank.