Resveratrol, Tween 80, and anhydrous ethanol were purchased from Macklin (Shanghai, China). Olive oil was obtained from local supermarkets. GOS was prepared from alginate-derived PG using alginate lyase, which was purified from Pseudoalteromonas sp. strain 272 (Li et al., 2021 ).
Resveratrol
Manufactured by Maclin Biochemical Technology
Sourced in China
Resveratrol is a natural compound found in various plants, including grapes, berries, and peanuts. It is a polyphenol with antioxidant properties. The core function of Resveratrol is to provide a purified extract of this compound for use in research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Nah2po4, by Tianjin Damao (3 mentions)
Kh2po4, by Tianjin Damao (3 mentions)
Fecl3, by Tianjin Damao (3 mentions)
Cinnamaldehyde, by Maclin Biochemical Technology (2 mentions)
Tween 80, by Maclin Biochemical Technology (1 mentions)
Eugenol, by Maclin Biochemical Technology (1 mentions)
Sodium caseinate, by Merck Group (1 mentions)
Polydatin, by Merck Group (1 mentions)
Tween 20, by Maclin Biochemical Technology (1 mentions)
Carvacrol, by Maclin Biochemical Technology (1 mentions)
Citral, by Maclin Biochemical Technology (1 mentions)
Osthole, by Maclin Biochemical Technology (1 mentions)
Pectin, by Maclin Biochemical Technology (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene fluoride pvdf membrane, by Merck Group (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
15 protocols using resveratrol
1
Galacto-oligosaccharide Synthesis from Alginate
2
Antimicrobial Activity of Natural Compounds on Listeria
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The bacterial strain L. monocytogenes CMCC 54004 (Lm 54004, serotype 1/2a) was purchased from the China National Center for Medical Culture Collections (CMCC), source from Czech Institute of Epidemiology and Microbiology. The strain was stored at −80°C in the brain heart infusion (BHI; Beijing Land Bridge Technology, China) with 25% (v/v) glycerol. The strain was activated by transferring 0.2 ml of the frozen culture into 20 ml of BHI and incubating at 37°C for 18 h with two consecutive transfers.
Four natural antimicrobial compounds: cinnamaldehyde, eugenol, resveratrol, and thymoquinone were obtained from Macklin, China; Solarbio, China and Yuanyeshengwu, China respectively. The control compound, sodium hypochlorite (10% active chlorine), was obtained from Sinopharm Chemical Reagent, China. The purity of all the compounds was above 98%. The four natural compounds were diluted with 1% dimethyl sulfoxide (DMSO) in BHI, with 1% DMSO shown not to exhibit an adverse effect on the growth of L. monocytogenes (Fan et al., 2018 (link)).
Four natural antimicrobial compounds: cinnamaldehyde, eugenol, resveratrol, and thymoquinone were obtained from Macklin, China; Solarbio, China and Yuanyeshengwu, China respectively. The control compound, sodium hypochlorite (10% active chlorine), was obtained from Sinopharm Chemical Reagent, China. The purity of all the compounds was above 98%. The four natural compounds were diluted with 1% dimethyl sulfoxide (DMSO) in BHI, with 1% DMSO shown not to exhibit an adverse effect on the growth of L. monocytogenes (Fan et al., 2018 (link)).
3
Bioactive Compound Enriched Emulsions
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Sodium caseinate, polydatin (HPLC grade, >95%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). WPI was purchased from Davisco International, Inc. (Russel, MN, USA). Tween 20 (Biotechnology grade, ≥99%), resveratrol (trans-isomer, ≥98%), fish oil and peppermint oil were respectively obtained from Macklin Co. (Shanghai, China), Sango Biotech Co. (Shanghai, China), Chaopu Co. (Shanghai, China), Ltd. and Zixin Biotechnology Co., Ltd. (Shanghai, China). Sunflower oil (Duoli brand) was purchased from a local market (Wuxi, China). MCT (C8:C10 = 60:40) was purchased from Yong sheng Industry and Trade Co., Ltd. (Guangzhou, China). Other materials of analytical grade were purchased from Sino-Pharm CNCM Ltd. (Shanghai, China).
4
Isolation and Characterization of Leaf Spot Pathogen
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In May 2021 (kiwifruit flowering period determined using the BBCH-scale), leaves of Actinidia deliciosa ‘Guichang’ visibly affected by leaf spot disease were collected from Xifeng County (27°2′ N, 106°30′ E). The leaves were disinfected with 75% alcohol for 30 s, rinsed with sterile water three times, and dried with sterile paper. The diseased tissue was then cut into 1 cm2 pieces, placed in potato dextrose agar (PDA: 200.0 g potato, 20.0 g glucose, 17.0 g agar/L) plates, and cultured at a constant temperature of 28 °C and 75% relative humidity for 5 d. A single colony was selected and transferred to new PDA plates until the mycelia were identical in colour and shape. The pure culture obtained was stored in 25% (volume/volume) glycerol at −80 °C for long-term storage. The natural antifungal agents (osthole, cinnamaldehyde, resveratrol, allicin, honokiol, citral, and carvacrol) used with purities of ≥98% were provided by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) and stored at 4 °C.
5
Resveratrol-Pectin Nanocarrier Delivery
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Resveratrol and pectin were purchased from Macklin Biochemical Co., Ltd., (Shanghai, China) and Sigma-Aldrich Corp. (St. Louis, MO, USA) provided zein. Corn oil was purchased from Wuhan Baixing Biological Technology Co., Ltd., (Wuhan, China). The dialysis membranes with 1000Da MWCO were obtained from Yuanye Biological Co., Ltd., (Shanghai, China). The reagents used in the experiment were of analytical grade.
6
Resveratrol's Anti-Cancer Effects
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Resveratrol was purchased from Macklin (Shanghai, China), Dulbecco's modified Eagle medium (DMEM), fetal bovine serum (FBS), phosphate buffered saline (PBS), 0.25% trypsin and penicillin-streptomycin were purchased from Gibco (GrandIsland, NY, USA), RIPA lysis buffer, methyl thiazolyl tetrazolium (MTT), BCA protein assay kits were purchased from Beyotime Institute of Biotechnology (Shanghai, China), β-actin and Beclin1 monoclonal antibodies were purchased from Santa Cruz Biotechnology (CA, USA), proliferating cell nuclear antigen (PCNA), cyclinD1, E-cadherin, N-cadherin, Vimentin, β-catenin, c-Myc, glycogen synthase kinase-3β (GSK-3β), p62 and LC3 were bought from CST (Danvers, MA, USA), Histone-H3 was bought from Wuhan Proteintech Group, Inc. (Wuhan, China), electrochemiluminescence (ECL) solution and polyvinylidene fluoride (PVDF) membrane were bought from Millipore (Billerica, MA, USA), 4',6-diamidino-2-phenylindole (DAPI) was bought from Google Biological Technology Co., Ltd. (Wuhan, China), and PHK67 dye was bought from Umibio Co., Ltd (Shanghai, China).
7
Cytotoxic Effects of Resveratrol, Methotrexate, Phenoxybenzamine, and Thiostrepton on A549 Cells
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A549 cells were cultured in DMEM (4.5 g/L D-glucose, Gibco, USA) containing 10% fetal bovine serum, and 5,000 cells per well were seeded in a 96-well plate. After 24 hours, all the cells were stuck to the wall. We further prepared a solution of resveratrol (Lot#:C12594500, Macklin, China), methotrexate (Lot#: C12150088, Macklin, China), phenoxybenzamine (Lot#: C11947938, Macklin, China), and thiostrepton. The preparation concentration was set as 1125 mg/mg mL in dimethyl sulfoxide (DMSO, Lot#: RNBH9957, Sigma, America), and then passed through a 0.22 μm filter membrane (Merck, America). The four drugs were added at concentrations of 0, 8, 32, 64, and 128 μmol/L for 48 hours and 72 hours, respectively. After treatmented, we discarded the supernatant, added 10% CCK-8 (Lot.SX749, Dojindo, Japan) and incubated for 2 hours, and then read the results with Multiskan GO 1510-03667C at a wavelength of 450 nm.
8
Evaluation of Bioactive Compounds
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Pterostilbene (PTE, purity ≥ 99.0%, Figure 1 A) was purchased from Dibai Biotechnology Co. (Shanghai, China), resveratrol (RE, purity ≥ 99.0%, Figure 1 B), camptothecin (CPT, purity ≥ 97.0%) and irinotecan (CPT-11, purity ≥ 99.0%) were purchased from Macklin Biochemical Co. (Shanghai, China), Furamidine dihydrochloride (FM, purity ≥ 98.0%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetonitrile and methyl alcohol were HPLC-grade from Merck (Kenilworth, NJ, USA). Isopropanol, ethanol and dimethylsulphoxide (DMSO) were from Sinopharm Chemical Reagent Co., LTD (Shanghai, China). Ultrapure water obtained from a Milli-Q system (Millipore, Bedford, MA, USA).
9
Resveratrol-Loaded Nanoparticles for Osteosarcoma
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Resveratrol was obtained from Macklin (Shanghai, China). DSPE-PEG2000, FA-PEG2000-DSPE was obtained from Ruixibio (Xi’an, China). Soy phosphatidylcholine, cholesterol, DiR iodide were purchased from Meilunbio (Dalian, China). Coumarin 6 (Cou-6) was obtained from Aladdin (Shanghai, China). JAK 2 antibody, P-STAT 3 antibody, Caspase 3 antibody, Cleaved Caspase 3 antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). P-JAK 2 antibody was acquired from Abcam (Cambridge, UK). STAT 3 antibody, BCL-2 antibody, Bax antibody, Cytochrome antibody, and N Tubulin β polyclonal antibody were obtained from Bioworld Technology (Nanjing, China). The peroxidase-conjugated secondary antibody was obtained from CWBIO (Taizhou, China).
Human osteosarcoma cell line 143B cells were obtained from the Nanfang Hospital, Southern Medical University (Guangzhou, China). Cells were cultured in DMEM medium containing 10% FBS in a 37°C incubator containing 5% CO2.
Male nude mice (6–8 weeks old) were purchased from Bestest (Zhuhai, China). All animal studies were conducted in accordance with the Guide for the Care and Use of Laboratory Animals. The Experimental Animal Ethics Committee of South China Hospital, South China University approved all experimental protocols.
Human osteosarcoma cell line 143B cells were obtained from the Nanfang Hospital, Southern Medical University (Guangzhou, China). Cells were cultured in DMEM medium containing 10% FBS in a 37°C incubator containing 5% CO2.
Male nude mice (6–8 weeks old) were purchased from Bestest (Zhuhai, China). All animal studies were conducted in accordance with the Guide for the Care and Use of Laboratory Animals. The Experimental Animal Ethics Committee of South China Hospital, South China University approved all experimental protocols.
10
Antimicrobial Activity Evaluation Protocol
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Chromeazurol S (CAS), hexadecyl trimethyl ammonium bromide (CTAB), and resveratrol were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Glucose, peptone, NaH2PO4, KH2PO4, and FeCl3 were purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). MgSO4·7H2O, CaCl2, Na2HPO4·12H2O, NaCl, NH4Cl, MeOH, and 95% EtOH were obtained from Xilong Science Co., Ltd. (Guangzhou, China). The phosphate buffer solution (PBS) and dimethyl sulfoxide (DMSO) were acquired from Biosharp (Anhui, China). Agar powder was bought from Guangdong Huankai Microbial Technology Co., Ltd. (Guangdong, China), and N-methyl-3-phenyl-2-propen-1-amine was bought from Sigma (MO, USA). (5Z)-4-bromo-5-(bromomethylene)-2(5H)-furanone was kindly presented by Prof. W.M. Chen (Jinan University, China). Hordenine, 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMN), and crystal violet were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Furthermore, P. aeruginosa PAO1 was obtained from Prof. Q.H. Gong (Ocean University of China, China).
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