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Cyt c from equine heart

Manufactured by Merck Group

Cyt c from equine heart is a laboratory product that contains cytochrome c, a heme-containing protein found in the mitochondria of eukaryotic cells. Cytochrome c is a key component of the electron transport chain and plays a critical role in cellular respiration. This product is derived from the heart tissue of horses and can be used in various biochemical and biophysical studies.

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2 protocols using cyt c from equine heart

1

Cyt c-HA-NH2 Conjugate for Cell Proliferation

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Cyt c from equine heart was from Sigma-Aldrich® (St. Louis, MO) and HA-NH2 (10 kDa) from Creative PEGWorks (Winston-Salem, NC). The dithiobis(succinimidyl propionate) (DSP) cross linker was obtained from ProteoChem™ (Loves Park, IL). The cell lines A549 (human lung adenocarcinoma; ATCC® CCL185 ™) and COS-7 (African green monkey kidney fibroblast) cells were from the American Type Culture Collection (Manassas, VA). CellTiter 96 aqueous non-radioactive cell proliferation assay was from Promega Corporation (Madison, WI). NucBlue® Fixed Cell ReadyProbes® Reagent (4′,6-diamidino-2-phenylindole, dihydrochloride, DAPI), fluorescein (FITC), Annexin Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and propidium iodide (PI) were purchased from Invitrogen (Carlsbad, CA). Caspase colorimetric assay kit was purchased from BioVision, Inc., San Francisco, CA and the caspase 9 substrate was from Sigma Aldrich (St. Louis, MO). All other chemicals (reagentor analytical grade) were purchased from various suppliers and used without further purification.
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2

Cyt c-HA-NH2 Conjugate for Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cyt c from equine heart was from Sigma-Aldrich® (St. Louis, MO) and HA-NH2 (10 kDa) from Creative PEGWorks (Winston-Salem, NC). The dithiobis(succinimidyl propionate) (DSP) cross linker was obtained from ProteoChem™ (Loves Park, IL). The cell lines A549 (human lung adenocarcinoma; ATCC® CCL185 ™) and COS-7 (African green monkey kidney fibroblast) cells were from the American Type Culture Collection (Manassas, VA). CellTiter 96 aqueous non-radioactive cell proliferation assay was from Promega Corporation (Madison, WI). NucBlue® Fixed Cell ReadyProbes® Reagent (4′,6-diamidino-2-phenylindole, dihydrochloride, DAPI), fluorescein (FITC), Annexin Dead Cell Apoptosis Kit with Annexin V Alexa Fluor® 488 and propidium iodide (PI) were purchased from Invitrogen (Carlsbad, CA). Caspase colorimetric assay kit was purchased from BioVision, Inc., San Francisco, CA and the caspase 9 substrate was from Sigma Aldrich (St. Louis, MO). All other chemicals (reagentor analytical grade) were purchased from various suppliers and used without further purification.
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