Sybr green pcr master mix
SYBR Green PCR Master Mix is a pre-mixed solution containing all the necessary components for real-time quantitative PCR, including SYBR Green I dye, DNA polymerase, dNTPs, and buffer. The mix is designed to provide reliable and sensitive detection of target DNA sequences.
Lab products found in correlation
30 protocols using sybr green pcr master mix
RNA Extraction and qRT-PCR Analysis
Quantifying Genomic DNA Methylation
Quantification of GLUT3 Expression
Quantitative RT-PCR Analysis of Key Cellular Regulators
Quantitative RT-PCR Analysis of RASSF8 and IL-6
RNA Extraction and qPCR Analysis
RNA Extraction and qRT-PCR Analysis
Quantitative RT-PCR Analysis of Antigen-Specific T-Cell Activation
Quantification of Mitochondrial DNA
“qPCR analysis for mitochondrial DNA” has been published previously [13 (link)][14 (link)],[15 (link)],. Total DNA was isolated using Quick-gDNA MiniPrep (Zymo Research, Cat. No. D3007). Mitochondrial DNA (mtDNA) was quantified with SYBR Green PCR Master Mix on Step One Plus, ABI instrument (Applied Biosystems, Grand Island, NY, USA). The relative mtDNA copy number was calculated from the subtraction of mtDNA copies to nuclear DNA copies. The relative fold change was then calculated based on the ΔΔCt method. Primers used in this study are shown in Table
Quantitative Real-Time PCR Analysis of mRNA Expression
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!