Plasma was analyzed for corticosterone levels by a competitive ELISA (Immunodiagnostic Systems, Fountain Hills, AZ)) according to the manufacturer’s direction. All samples were run on one 96-well plate. Prolactin hormone was determined in plasma by ELISA (Alpco Immunoassays, Salem, NH). Twenty-five microliters of plasma was added to the microplate coated with a monoclonal anti-rat prolactin antibody. A standard curve between 5 and 80 ng/ml of prolactin was utilized to compare the content of prolactin in the plasma samples.
Competitive elisa
Manufactured by Immunodiagnostic Systems
Sourced in Azerbaijan
The Competitive ELISA is a type of enzyme-linked immunosorbent assay (ELISA) used for the quantitative detection of specific analytes in a sample. The core function of the Competitive ELISA is to measure the concentration of an analyte by competition between the analyte in the sample and a known amount of analyte-specific antibody or antigen.
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Lab products found in correlation
2 protocols using competitive elisa
1
Plasma Corticosterone and Prolactin Assays
2
Serum Calcium and Phosphate Analysis
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All mice were fasted for 2 hours in the morning, after which serum from mice was obtained by cheek bleed. This procedure was performed under isoflurane anesthesia to minimize suffering. Serum calcium and phosphate were measured colorimetrically using kits from Stanbio (Boerne, TX) and Abcam (Cambridge, MA), respectively. Serum PTH and FGF23 were analyzed using the Mouse Intact PTH 1–84 Kit and Mouse Intact FGF23 ELISA Kit, respectively (Immutopics, San Clemente, CA). Serum 1,25D was immunopurified and measured by competitive ELISA (Immunodiagnostic Systems, Gaithersburg, MD).
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