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His6 her

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His6-HER is a recombinant protein that contains a hexahistidine (His6) tag. The His6 tag allows for easy purification of the protein using affinity chromatography.

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Lab products found in correlation

Gst llgl2, by Abnova (2 mentions) Gst mst1, by Abnova (2 mentions) Bcar3, by Abnova (2 mentions) Gst rassf1, by Abnova (2 mentions) Flag nsun6, by OriGene (2 mentions) Flag sav1, by OriGene (2 mentions) Flag mst1, by OriGene (2 mentions) Flag ror1, by OriGene (2 mentions) Flag her3, by OriGene (2 mentions) Hispur cobalt spin column, by Thermo Fisher Scientific (2 mentions) Pierce gst spin purification kit, by Thermo Fisher Scientific (2 mentions) Gst ror1, by Sino Biological (2 mentions)

2 protocols using his6 her

1

Purification and Characterization of Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
GST-LLGL2, GST-MST1, BCAR3 and GST-RASSF1 were obtained from Abnova. FLAG-NSUN6, FLAG-SAV1, FLAG-MST1, FLAG-ROR1 (WT and extracellular domain), and FLAG-HER3 were obtained from Origene Technologies. His6-MOB1 was purchased from Novoprotein Inc. GST-ROR1 was obtained from SignalChem. His6-HER3 intracellular domain (ICD) and WT His6-MST1 and corresponding mutants were expressed in E.coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using HisPur™ Cobalt Spin Columns (Life Technologies). Domain deletion or point mutants of GST-ROR1 ICD and GST-LLGL2 were purified using Pierce™ GST Spin Purification Kit (Life Technologies). Biotinylated HER3 and phospho-HER3 peptides were synthesized from Elim Biopharmaceuticals Inc.
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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2

Purification and Characterization of Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
GST-LLGL2, GST-MST1, BCAR3 and GST-RASSF1 were obtained from Abnova. FLAG-NSUN6, FLAG-SAV1, FLAG-MST1, FLAG-ROR1 (WT and extracellular domain), and FLAG-HER3 were obtained from Origene Technologies. His6-MOB1 was purchased from Novoprotein Inc. GST-ROR1 was obtained from SignalChem. His6-HER3 intracellular domain (ICD) and WT His6-MST1 and corresponding mutants were expressed in E.coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using HisPur™ Cobalt Spin Columns (Life Technologies). Domain deletion or point mutants of GST-ROR1 ICD and GST-LLGL2 were purified using Pierce™ GST Spin Purification Kit (Life Technologies). Biotinylated HER3 and phospho-HER3 peptides were synthesized from Elim Biopharmaceuticals Inc.
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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