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Nis a n sim analysis module

Manufactured by Nikon
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The NIS-A N-SIM analysis module is a component of Nikon's imaging software suite. Its core function is to provide analysis capabilities for microscopy data, without interpretation or extrapolation.

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Lab products found in correlation

Apo tirf objective, by Oxford Instruments (2 mentions) High precision coverslips, by Zeiss (2 mentions) Xyla scmos camera, by Oxford Instruments (2 mentions) Ti microscope, by Nikon (2 mentions)

2 protocols using nis a n sim analysis module

1

Chromaffin Cell Imaging Protocol

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Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
Logan T., Bendor J., Toupin C., Thorn K, & Edwards R.H. (2017). α-Synuclein Promotes Dilation of the Exocytotic Fusion Pore. Nature neuroscience, 20(5), 681-689.
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2

Chromaffin Cell Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated chromaffin cells were plated onto high precision coverslips (Zeiss), fixed 3–5 days later and immunostained as described above. Samples were imaged on a Nikon Ti microscope equipped with a 100× 1.49 NA Apo TIRF objective and an Andor Xyla sCMOS camera. Structured illumination images were acquired as a 15-slice z-stack with 120 nm step size and reconstructed using Nikon Elements software outfitted with the NIS-A N-SIM analysis module. Colocalization was quantified with the Coloc2 module in ImageJ.
Logan T., Bendor J., Toupin C., Thorn K, & Edwards R.H. (2017). α-Synuclein Promotes Dilation of the Exocytotic Fusion Pore. Nature neuroscience, 20(5), 681-689.
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