Au2700 automatic biochemistry analyzer

SCr and other biochemical indicators were measured. Fasting blood specimens were collected prior to and at 24 and 48 h after the procedure in the biochemical laboratory and subjected to analysis using an Olympus AU2700 Automatic Biochemistry Analyzer (Olympus, Center Valley, PA, USA) for determination. Urine specimens were collected prior to and at 2, 6, 12, 24 and 48 h after the procedure and immediately centrifuged (1,409 × g, 20 min, 4°C). The supernatant fractions were collected and stored frozen at −80°C until use. Urinary levels of IL-18 were measured using an ELISA kit purchased from R&D Systems (Minneapolis, MN, USA). All procedures were performed strictly following the manufacturer’s instructions. Renal function was assessed by the eGFR using the Modification of Diet in Renal Disease formula for Chinese patients (8 (link)): GFR (ml/min/1.73 m²) = 175 × SCr (mg/dl)^−1.1549 × age^−0.2039 (x 0.79 if female). This equation gives a more accurate assessment of renal function than SCr alone. CIN was defined as an increase of ≥0.5 mg/dl or ≥25% in SCr concentration over baseline 24–48 h after the intravascular administration of contrast medium, without an alternative etiology (9 (link)).

Venous blood was collected from all rats, and red blood cells (RBC), white blood cells (WBC), and hemoglobin (HGB) were determined using an XFA6100 automatic hemocyte analyzer (Perlong Medical Inc, China). Blood oxygen saturation (S_cO₂) was measured using a TUFFSAT oximeter (Ohmeda medicalMedical Inc, USA). Albumin (ALB) and bilirubin (BIL) were determined by an AU2700 automatic biochemistry analyzer (Olympus Inc, Japan).