Wild-type male C57BL/6J mice, IL-6KO mice, and RAG-/- mice were purchased from The Jackson Laboratory (Bar Harbor, ME). All gene-deficient mice used are on the C57BL/6 background. IFNAR-/-48 were provided by Dr. Paul W. Dempsey (Department of Microbiology and Molecular Genetics, University of California, Los Angeles) and Dr. Tadatsugu Taniguchi (Department of Immunology, Tokyo University, Japan) to Dr. W. Overwijk and crossed to the C56BL/6 background. IL15Rα-/- mice49 were originally generated by and obtained from Dr. Averil Ma through Dr. Leo Lefrancois and crossed to the C57BL/6 background. All animal experiments were performed according to the institutional guidelines for the care and use of laboratory animals.
Il 6 ko mice
Manufactured by Jackson ImmunoResearch
IL-6–KO mice are genetically modified mice that have a targeted deletion of the interleukin-6 (IL-6) gene. These mice lack the expression of the IL-6 cytokine, which is a key regulator of inflammation and immune response.
Automatically generated - may contain errors
Lab products found in correlation
Rag mice, by Jackson ImmunoResearch (1 mentions)
Wild type c57bl 6j male mice, by Jackson ImmunoResearch (1 mentions)
Rag2 il2rg dko mice, by Taconic Biosciences (1 mentions)
Laminin, by Merck Group (1 mentions)
Calcein, by Merck Group (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
Gapdh, by Novus Biologicals (1 mentions)
Lamin a c, by Active Motif (1 mentions)
P nf κb p65, by Santa Cruz Biotechnology (1 mentions)
Lamin b1, by Abcam (1 mentions)
Nf κb p65, by Cell Signaling Technology (1 mentions)
β actin, by Merck Group (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
C57bl 6, by Jackson ImmunoResearch (1 mentions)
4 protocols using il 6 ko mice
1
Mouse Strains for Immunology Research
2
Generation and Characterization of Transgenic Mice
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C57BL/6 (WT) and IL-6 KO mice were purchased from The Jackson Laboratory. RAG2/IL2rg DKO mice were purchased from Taconic Farms. IL-21R–deficient mice (Ozaki et al., 2002 (link)), IL-23R-GFP reporter mice (Awasthi et al., 2009 (link)), STAT-3–deficient mice (Lee et al., 2002 (link)), and IL-17A/F–deficient mice (Ogura et al., 2008 (link); Ishigame et al., 2009 (link)) were generated and provided by the referenced collaborators. Crosses of these various strains were generated and maintained in the animal facilities of the National Institutes of Health (NIH) in Bethesda or Frederick, MD, in full compliance with institutional guidelines. Male and female mice aged 6 to 12 wk were used for analyses. Littermate controls were used for all experiments with STAT-3 KO mice. TKO mice were derived from the respective single KO mice and bred homozygously. Their controls were in-house WT mice. All studies were performed under specific pathogen–free (SPF) conditions in compliance with NIH institutional guidelines, under IACUC-approved protocols (NEI-581 and NIAID/LCID 8E).
3
Muscle and Bone-Specific Lmna Knockout Mice
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The Lmnaf/f mice, kindly provided by Dr. Yixian Zhen (Department of Embryology, Carnegie Science), were crossed with HSA-Cre (from the Jackson Laboratory, catalog #006149) or Ocn-Cre (kindly provided by Dr. Tom Clemens, Johns Hopkins Medical School) transgenic mice to generate muscle- or OB-selective cko mutant mice, LmnaHSA-cko or LmnaOcn-cko, respectively. The mutant mice were in the C57BL/6J mouse background (for more than six generations). The IL-6–KO mice, which are in the C57BL/6J background, were purchased from the Jackson Laboratory (catalog #002650). The IL-6–KO homozygous mice were crossed with Lmnaf/w;HSA-Cre mice to get the heterozygous mice, and the double-KO mice were obtained from the next breeding generation.
Mouse monoclonal antibodies, including lamin A/C (39287, ACTIVE MOTIF), β-actin (A1978, Sigma), GAPDH (NB300-221, Novus), IL-6 (MAB406, R&D), NF-κB p65 (#8242, Cell Signal), p-NFκB p65 (sc-135769, Santa Cruz), p-IκBα (#2859, Cell Signaling), and IκBα (#4814, Cell Signaling), were used. Rabbit polyclonal antibody laminin (L9393, Sigma) and lamin B1 (ab16048, Abcam) were used. Secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. Calcein (C0875) was obtained from Sigma. Other chemicals and reagents used in this study were of analytical grade.
Mouse monoclonal antibodies, including lamin A/C (39287, ACTIVE MOTIF), β-actin (A1978, Sigma), GAPDH (NB300-221, Novus), IL-6 (MAB406, R&D), NF-κB p65 (#8242, Cell Signal), p-NFκB p65 (sc-135769, Santa Cruz), p-IκBα (#2859, Cell Signaling), and IκBα (#4814, Cell Signaling), were used. Rabbit polyclonal antibody laminin (L9393, Sigma) and lamin B1 (ab16048, Abcam) were used. Secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. Calcein (C0875) was obtained from Sigma. Other chemicals and reagents used in this study were of analytical grade.
4
Generation and Characterization of Transgenic Mice
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The mDia1/miR-146a DKO mice have been described previously (23 (link)). In brief, Mir146a–/– mice in a C57BL/6 background purchased from The Jackson Laboratory (stock 016239) were crossed with mDia1-deficient mice to generate Diap1–/– Mir146a–/– mice (DKO mice) (6 (link)). To generate Diap1–/– Mir146a–/– Il6–/– TKO mice, IL-6–KO mice purchased from The Jackson Laboratory (stock 002650) were crossed with DKO mice. CD45.1 congenic mice were purchased from Charles River Laboratories (B6-LY-5.12/Cr, strain code 564).
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