Log In Sign up
The largest database of trusted experimental protocols

Ab391

Manufactured by Abcam
Visit Supplier

Ab391 is a primary antibody that targets the human GAPDH protein. It is a rabbit polyclonal antibody produced using a synthetic peptide conjugated to KLH as the immunogen. The antibody is provided in liquid form and can be used for applications such as Western blotting, immunohistochemistry, and immunocytochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Enrich sec 650 10 300 column, by Bio-Rad (1 mentions) Ab205718, by Abcam (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions)

2 protocols using ab391

1

Characterizing Lysozyme-LPS Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gel filtration chromatography was carried out using an Enrich SEC 650 (10/300) column (Bio Rad) [18 (link),22 (link)]. For analysis, 500 μL of lysozyme (500 μg/mL) and 500 μL of LPS (500 μg/mL) were mixed and then incubated for 30 min at 37 °C at room temperature, and applied to the column at a flow rate of 1.0 mL/min. The elution profile was analyzed by monitoring A215, and by SDS-PAGE and western blot analyses of the fractions using an affinity-purified anti-lysozyme antibody (1:500 dilution, ab391, Abcam).
Bao Y., Zhang H., Huang X., Ma J., Logue C.M., Nolan L.K, & Li G. (2018). O-specific polysaccharide confers lysozyme resistance to extraintestinal pathogenic Escherichia coli. Virulence, 9(1), 666-680.
+ Open protocol
+ Expand
2

Western Blot Analysis of RBC Ghosts

Check if the same lab product or an alternative is used in the 5 most similar protocols
RBCs isolated from each recipient group 2 days after immunization with anti-HEL monoclonal antibodies were lysed in 5 mmol/L sodium phosphate (pH 7.5) with protease inhibitor cocktail (Sigma Roche) at 4°C and centrifuged at 14,000 rpm, as outlined previously.29 (link),48 (link) RBC ghosts were then subjected to western blot analysis with rabbit polyclonal anti-HEL (Abcam, ab391, 1:5,000) and detection with goat anti-rabbit horseradish peroxidase (Abcam, ab205718 1:10,000). Mouse monoclonal anti–glyceraldehyde 3-phosphate dehydrogenase (clone: GA1R, 1:1,000), followed by goat anti-mouse IgG1 horseradish peroxidase (Bethyl Laboratories, 1:10,000) was applied following stripping membranes with 25 mM glycine hydrochloride with 1% sodium dodecyl sulfate, pH 2. All membranes were developed using a chemiluminescence reagent (HyGlo ECL, Denville Scientific Inc.).
Mener A., Patel S.R., Arthur C.M, & Stowell S.R. (2018). Antibody-mediated immunosuppression can result from RBC antigen loss independent of Fcγ receptors in mice. Transfusion, 59(1), 371-384.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!