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Sc 374280

Manufactured by Merck Group
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The Sc-374280 is a laboratory equipment product from Merck Group. It is a compact and versatile device designed for precise measurements and analysis in various scientific applications. The core function of Sc-374280 is to provide accurate and reliable data collection and processing capabilities for researchers and laboratory professionals.

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Lab products found in correlation

Spa 810, by PhosphoSolutions (2 mentions) H00056339 b01p, by Merck Group (2 mentions) Hpa038002, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 488 donkey anti mouse secondary antibody, by Thermo Fisher Scientific (2 mentions) Alexa fluor 546 donkey anti rabbit secondary antibody, by Thermo Fisher Scientific (2 mentions) Abe572, by Merck Group (2 mentions)

2 protocols using sc 374280

1

HeLa and MEF Cells for m6A Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa (cervical cancer) was originally purchased from ATCC and MEF cells were a gift from D.J. Kwiatkowski (Harvard Medical School). Cells were not authenticated recently but tested negative for mycoplasma contamination. Both cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum (FBS). Antibodies used in the experiments are listed below: anti-YTHDF2 (Proteintech 24744-1-AP, 1:1000 WB, 1:600 IF); anti-Hsp70 (Stressgen SPA-810, 1:1000 WB); anti-FTO (Phosphosolutions 597-Fto, 1:1000 WB, 1:600 IF); anti-METTL3 (Abnova H00056339-B01P, 1:1000 WB, 1:600 IF); anti-METTL14 (sigma HPA038002, 1:1000 WB, 1:600 IF); anti-WTAP (Santa Cruz sc-374280, 1:1000 WB, 1:600 IF); anti-m6A (Millipore ABE572, 1:1000 m6A immunoblotting); Alexa Fluor 488 donkey anti-mouse secondary antibody (Invitrogen A21202. 1:600 IF); Alexa Fluor 546 donkey anti-rabbit secondary antibody (Invitrogen A10040, 1:600 IF).
Zhou J., Wan J., Gao X., Zhang X, & Qian S.B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.
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2

HeLa and MEF Cells for m6A Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa (cervical cancer) was originally purchased from ATCC and MEF cells were a gift from D.J. Kwiatkowski (Harvard Medical School). Cells were not authenticated recently but tested negative for mycoplasma contamination. Both cells were maintained in Dulbecco's Modified Eagle's Medium (DMEM) with 10% fetal bovine serum (FBS). Antibodies used in the experiments are listed below: anti-YTHDF2 (Proteintech 24744-1-AP, 1:1000 WB, 1:600 IF); anti-Hsp70 (Stressgen SPA-810, 1:1000 WB); anti-FTO (Phosphosolutions 597-Fto, 1:1000 WB, 1:600 IF); anti-METTL3 (Abnova H00056339-B01P, 1:1000 WB, 1:600 IF); anti-METTL14 (sigma HPA038002, 1:1000 WB, 1:600 IF); anti-WTAP (Santa Cruz sc-374280, 1:1000 WB, 1:600 IF); anti-m6A (Millipore ABE572, 1:1000 m6A immunoblotting); Alexa Fluor 488 donkey anti-mouse secondary antibody (Invitrogen A21202. 1:600 IF); Alexa Fluor 546 donkey anti-rabbit secondary antibody (Invitrogen A10040, 1:600 IF).
Zhou J., Wan J., Gao X., Zhang X, & Qian S.B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594.
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