The chemical compositions of the EOs of C. goetheanus (A, B, and C), were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan) a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C), and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [6 (link),88 (link),89 (link),90 (link)]). The components were quantified using gas chromatography (GC) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before, except for the carrier hydrogen gas. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (St. Louis, MI, USA), according with Van den Dool and Kratz [91 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [32 ,33 ].
N alkanes c8 c40
Manufactured by Merck Group
Sourced in United States
N-alkanes (C8–C40) are a class of saturated aliphatic hydrocarbons with the general formula CnH2n+2, where n represents the number of carbon atoms in the molecule. This range of n-alkanes spans from octane (C8) to tetracontane (C40). These compounds are commonly used as standards, references, and calibration materials in various analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Qp 2010 system, by Shimadzu (6 mentions)
Gc ms, by Shimadzu (5 mentions)
Rtx 5ms capillary column, by Restek (5 mentions)
Qp2010, by Shimadzu (4 mentions)
Qp2010 plus, by Shimadzu (2 mentions)
Infinitylab poroshell 120 ec c18 analytical column, by Agilent Technologies (1 mentions)
Hp 5 capillary column, by Agilent Technologies (1 mentions)
Sugar standards, by Merck Group (1 mentions)
Db 5 ms silica capillary column, by Shimadzu (1 mentions)
Qp 2010 plus system, by Shimadzu (1 mentions)
Isobutyl acetate, by Merck Group (1 mentions)
Epicatechin gallate, by Yuanye Bio-Technology (1 mentions)
Butyl acetate, by Merck Group (1 mentions)
Ethyl butanoate, by Merck Group (1 mentions)
Ethyl caproate, by Merck Group (1 mentions)
Citronellol, by Merck Group (1 mentions)
Acetic acid, by Yuanye Bio-Technology (1 mentions)
Vanillin, by Yuanye Bio-Technology (1 mentions)
Ethyl propanoate, by Merck Group (1 mentions)
Propyl acetate, by Merck Group (1 mentions)
11 protocols using n alkanes c8 c40
1
GC-MS Analysis of C. goetheanus EOs
2
Chemical Composition Analysis of Basil Essential Oil
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The chemical compositions of the EO of O. basilicum var. minimum were analyzed as reported by our research group [52 (link),53 (link)], using a Shimadzu QP-2010 (Kyoto, Japan) plus gas chromatography system equipped with an Rtx-5MS capillary column (Restek Corporation, Bellefonte, PA, USA) (30 m × 0.25 mm; 0.25 µm film thickness) coupled with a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan) and the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID). The program temperature and injection were the same operating conditions as described in the literature [54 (link),55 ], except for the carrier hydrogen gas, under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (San Luis, CA, USA), according to van den Dool and Kratz [56 (link)]. The components were identified by comparison of: (i) the experimental mass spectra with those compiled in libraries, and (ii) their retention indices to those found in the literature [57 ].
3
Chemical Profiling of Essential Oils
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The chemical compositions of the EOs of M. multiflora (A, B and C) and E. florida, were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan), a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The programmed temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C) and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [62 (link)]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40), Sigma-Aldrich (San Luis, MO, USA), according to van den Dool and Kratz [63 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [38 ,39 ].
4
GC-MS Analysis of Eucalyptus florida Essential Oils
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The chemical compositions of the EOs of E. florida (A and B), were analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan) a gas chromatography system equipped with an Rtx-5MS capillary column (30 m × 0.25 mm; 0.25 µm film thickness) (Restek Corporation, Bellefonte, PA, USA) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C) and a splitless injection (1 μL of a 2:1000 hexane solution), using the same operating conditions as described in the literature [67 (link),68 (link)]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (San Luis, USA), according with Van den Dool and Kratz [69 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [28 ,70 ,71 ].
5
GC-MS Analysis of Melaleuca Paivae Essential Oil
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The chemical composition of the EO of M. Paivae was analyzed using a Shimadzu QP-2010 plus (Kyoto, Japan), a gas chromatography system equipped with a capillary column (30 m × 0.25 mm; 0.25 µm film thickness) coupled with a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The programed temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C), and a splitless injection (1 μL of a 2:10 hexane solution), using the same operating conditions as described in the literature [3 (link),4 (link),5 (link)]. The components were quantified using gas chromatography (GC) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID) (Kyoto, Japan), under the same operating conditions as before, except the carrier was hydrogen gas. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (St. Louis, MO, USA), in accordance with Van den Dool and Kratz [41 (link)]. The components were identified by comparison (i) of the experimental mass spectra with those compiled in libraries (reference) and (ii) their retention indices to those found in the literature [19 ,20 ].
6
Characterization of UDMH-Containing Waste Mixture
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A blend of n-alkanes C8–C40 (0.5 mg/mL of each component in dichloromethane, Sigma-Aldrich, St. Louis, MO, USA) was used for the determination of RI. MS-grade acetonitrile (AppliChem, Darmstadt, Germany) was used for sample dilution and HPLC-HRMS. The following chromatographic columns (polar stationary phase and non-polar stationary phase, respectively) were used for GC-MS: SH-Stabilwax capillary column (30 m × 0.25 mm × 0.10 μm, Shimadzu Corporation, Kyoto, Japan) and HP-5 capillary column (30 m × 0.32 mm × 0.25 μm, Agilent, Santa Clara, CA, USA). An InfinityLab Poroshell 120 EC-C18 analytical column (4.6 × 100 mm, particle size 2.7 μm, Agilent, USA) was used for HPLC-HRMS.
A representative sample (0.1 g, dark resinous liquid) was taken from a mixture formed from the long-term storage of UDMH-containing waste [16 (link),17 (link),29 (link)]. The sample was diluted in 1 mL of acetonitrile and centrifuged for 20 min. The supernatant was injected into GC-MS (with the polar stationary phase).
A representative sample (0.1 g, dark resinous liquid) was taken from a mixture formed from the long-term storage of UDMH-containing waste [16 (link),17 (link),29 (link)]. The sample was diluted in 1 mL of acetonitrile and centrifuged for 20 min. The supernatant was injected into GC-MS (with the polar stationary phase).
7
Quantitative Analysis of Organic Compounds
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Sugar standards (sucrose, glucose, and fructose) and n-alkanes (C8–C40) were purchased from Sigma Corporation (Shanghai, China). (s)-(+)-2-Octanol was purchased from Alfa Aesar Corporation (Shanghai, China). Mixed amino acids standard solution was obtained from Wako Pure Chemical Industries, Limited. (Tokyo, Japan, 99.9%). Sodium chloride were purchased from Kelong Chemical Industry (Chengdu, China).
8
GC-MS Analysis of Essential Oils
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The chemical compositions of the EOs of E. patrisii, E. punicifolia, and M. tomentosa, were analyzed using a Shimadzu QP-2010 (Kyoto, Japan) plus gas chromatography system equipped with an Rtx-5MS capillary column (Restek Corporation, Bellefonte, PA USA) (30 m × 0.25 mm; 0.25 μm film thickness) coupled to a mass spectrometer (GC/MS) (Shimadzu, Kyoto, Japan). The program temperature was maintained at 60–240 °C at a rate of 3 °C/min, with an injector temperature of 250 °C, helium as the carrier gas (linear velocity of 32 cm/s, measured at 100 °C) and a splitless injection (1 μL of a 2:1000 hexane solution) using the same operating conditions as described in the literature [59 (link),60 (link)]. Except for the carrier hydrogen gas, the components were quantified using gas chromatography (CG) on a Shimadzu QP-2010 system (Kyoto, Japan), equipped with a flame ionization detector (FID), under the same operating conditions as before. The retention index for all volatile constituents was calculated using a homologous series of n-alkanes (C8–C40) Sigma-Aldrich (San Luis, AZ, USA), according Van den Dool and Kratz [61 (link)]. The components were identified by comparison i) of the experimental mass spectra with those compiled in libraries (reference) and ii) their retention indices to those found in the literature [51 ,52 ,53 ].
9
GC-MS Analysis of Essential Oils
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The chemical composition of the EOs was analyzed in the Adolpho Ducke laboratory of the Goeldi Museum (LAD/MPEG, Belém, Brazil) by gas chromatography coupled to mass spectrometry (GC/MS) in a SHIMADZU QP Plus-2010 system equipped with a DB-5MS silica capillary column (30 m × 0.25 mm; 0.25 m film thickness) under the following operating conditions: carrier gas: helium, linear velocity of 36.5 cm.s−1; injection mode: splitless (2 µL of oil in 0.5 mL of hexane); injector temperature: 250 °C, temperature program: 60–250 °C with a gradient of 3 °C.min−1; temperature of the ion source and other parts: 220 °C.
The quadrupole scan rate was 39 to 500 daltons.s−1. Ionization was performed in electron impact mode at 70 eV. The identification of volatile components was based on the linear retention index (RI) and the fragmentation patterns in the mass spectra by comparison with standard samples in the NIST-11 and FFNSC-2 databases and from the literature [41 ]. The RIs were obtained using the homologous series of n-alkanes (C8-C40) from Sigma–Aldrich (San Luis, AZ, USA).
The quadrupole scan rate was 39 to 500 daltons.s−1. Ionization was performed in electron impact mode at 70 eV. The identification of volatile components was based on the linear retention index (RI) and the fragmentation patterns in the mass spectra by comparison with standard samples in the NIST-11 and FFNSC-2 databases and from the literature [41 ]. The RIs were obtained using the homologous series of n-alkanes (C8-C40) from Sigma–Aldrich (San Luis, AZ, USA).
10
Analytical Standards for Metabolomics
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The organic acid standards (L-tartaric acid, lactic acid, malic acid, acetic acid, etc.), as well as polyphenol standards (protocatechuic acid, p-hydroxybenzonic acid, chlorogenic acid, vanillic acid, (-)-epigallocatechin gallate, epicatechin, vanillin, p-coumaric acid, (-)-epicatechin gallate, isoferulic acid, etc.), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Water was purified using a Milli-Q system from Millipore (Bedford, MA, USA).
Isoamyl ethanoate, benzylcarbinyl acetate, isobutyl acetate, butyl acetate, propyl acetate, hexyl acetate β-damascenone, naphthalene, cinnamene, linalool, citronellol, d-limonene, α-terpineol, nerol oxide, ethyl dodecylate, ethyl heptoate, ethyl caproate, ethyl butanoate, ethyl caprylate, ethyl propanoate, ethyl isobutyrate, ethyl n-valerate, etc., were provided by Sigma-Aldrich (Shanghai, China). The internal standard of 4-methyl-1-pentanol was obtained from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). The calculation of retention indices (RIs) was used with n-alkanes (C8-C40) purchased from Supelco (Bellefonte, PA, USA).
Isoamyl ethanoate, benzylcarbinyl acetate, isobutyl acetate, butyl acetate, propyl acetate, hexyl acetate β-damascenone, naphthalene, cinnamene, linalool, citronellol, d-limonene, α-terpineol, nerol oxide, ethyl dodecylate, ethyl heptoate, ethyl caproate, ethyl butanoate, ethyl caprylate, ethyl propanoate, ethyl isobutyrate, ethyl n-valerate, etc., were provided by Sigma-Aldrich (Shanghai, China). The internal standard of 4-methyl-1-pentanol was obtained from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). The calculation of retention indices (RIs) was used with n-alkanes (C8-C40) purchased from Supelco (Bellefonte, PA, USA).
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