Mouse monoclonal to vimentin

Primary antibodies used were: mouse monoclonal to vimentin (abcam, Cambridge, UK, Cat # ab 8069; 1:2000 dilution); rabbit polyclonal to GAPDH (abcam, Cambridge, UK, Cat # ab9485; 1:2000 dilution), lamin B2 (Thermofisher, Oxford, UK, Cat # PA5-29121, 1:500), CDH5 (abcam, Cambridge, UK, Cat # ab33168; 1:1000); rabbit monoclonal to laminA/C (Thermofisher, Oxford, UK, Cat # MA5-35284; 1:1000); lamin B1 (Thermofisher, Oxford, UK, Cat # 702972; 1:500). Secondary antibodies used were: goat anti-mouse IgG peroxidase conjugated (GE healthcare, Lisburn, UK, Cat # NA931V, 1:1000 dilution), donkey anti-Rabbit IgG peroxidase conjugated (GE healthcare, Lisburn, UK, Cat # NA934; 1:1000 dilution).

Proteins extracted from HCC cells and samples were applied to detect RNF187 protein expression by western blotting as previously described [32 (link)]. The polyclonal rabbit anti-RNF187 (1:1000; Abcam, USA), polyclonal rabbit anti-JunD (1:500; Abcam, USA), Rat monoclonal to E-cadherin (1:5000; Abcam, USA), Rabbit polyclonal to snail (1:500; Abcam, USA), and Mouse monoclonal to Vimentin (1:1000; Abcam, USA) antibodies were used as the primary antibody, respectively. GAPDH (1:5,000; Chemicon, USA) was used as the internal control.
Polyclonal rabbit anti-human RNF187 (1:100, Abnova, China), Rat monoclonal to E-cadherin (1:500), rabbit polyclonal to snail (1:100), and Mouse monoclonal to Vimentin (1:100) antibodies were used for immunofluorescence. Nuclei were counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA).