Cultures were prepared and maintained as described previously (Jaklitsch 2009 (link)) except that 2 % malt extract agar (MEA; 2 % w/v malt extract, 2 % w/v agar-agar; Merck, Darmstadt, Germany) was used as the isolation medium. Cultures used for the determination of growth rates and study of asexual morph micro-morphology were grown on CMD, 2 % MEA or potato dextrose agar (PDA, 39 g/l; Merck, Darmstadt, Germany) at 22–25 °C in darkness. Microscopic observations were made in tap water except where noted. Morphological analyses of microscopic characters were carried out as described earlier (Jaklitsch 2009 (link)). Data were gathered using a Nikon Coolpix 995 or Coolpix 4500 or a Nikon DS-U2 digital camera and measured with NIS-Elements D v. 3.0, or with a Zeiss Axiocam 506 colour digital camera and measured with Zeiss ZEN Blue Edition software. Methods of microscopy included stereomicroscopy using an Olympus SZ 60 or Nikon SMZ 1500 and Nomarski differential interference contrast (DIC) using the compound microscopes Nikon Eclipse E600 or Zeiss Axio Imager.A1. For certain images of ascomata the stacking software Zerene Stacker v. 1.04 (Zerene Systems LLC, Richland, WA, USA) was used. Measurements are reported as maximum and minimum in parentheses and the range representing the mean plus and minus the standard deviation of a number of measurements given in parentheses.
Sz 60
Manufactured by Nikon
The Nikon SZ 60 is a stereo zoom microscope designed for laboratory applications. It features a zoom range of 6.3x to 40x magnification and a working distance of 60 mm. The SZ 60 utilizes a binocular observation tube and halogen illumination system.
Automatically generated - may contain errors
Lab products found in correlation
Coolpix 4500, by Nikon (2 mentions)
Coolpix 995, by Nikon (2 mentions)
Ds u2, by Nikon (2 mentions)
Axio imager a1, by Zeiss (1 mentions)
Zen blue edition software, by Zeiss (1 mentions)
Axiocam 506, by Zeiss (1 mentions)
Eclipse e600, by Nikon (1 mentions)
Smz1500, by Nikon (1 mentions)
Eclipse 80i, by Nikon (1 mentions)
Photo paint x7, by Corel (1 mentions)
Draw x7, by Corel (1 mentions)
3 protocols using sz 60
1
Fungal Cultivation and Microscopic Analysis
2
Fungal Culture Preparation and Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cultures were prepared and maintained as described previously (Jaklitsch 2009 (link)) except that 2 % malt extract agar (MEA; 2 % w/v malt extract, 2 % w/v agar-agar; Merck, Darmstadt, Germany) was used as the isolation medium. Cultures used for the determination of growth rates and study of asexual morph micro-morphology were grown on 2 % MEA or potato dextrose agar (PDA, 39 g/l; Merck, Darmstadt, Germany) at room temperature, defined here as 22 ± 3 °C, or at 25 °C under alternating 12 h cool daylight and 12 h darkness. Microscopic observations were generally made in 3 % KOH, rarely in water or lactic acid where noted, and amyloidity was checked with Lugol solution. Morphological analyses of microscopic characters were carried out as described earlier (Jaklitsch 2009 (link)). Data were gathered using a Nikon Coolpix 995 or Coolpix 4500 or a Nikon DS-U2 digital camera and measured by using NIS-Elements D v. 3.0 software. Methods of microscopy included stereomicroscopy using an Olympus SZ 60 or Nikon SMZ 1500 and Nomarski differential interference contrast (DIC) using the compound microscope Nikon Eclipse E600. Kornerup & Wanscher (1981) was used as the colour standard.
3
Larvae and Pupae Microscopic Examination
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The preparation of the slide-mounted material basically followed May (1994) (link). The larvae chosen for microscopic examination were first dissected (the head, mouthparts, and body were separated), then cleared in 10% potassium hydroxide (KOH), rinsed in distilled water, and mounted on permanent microscope slides in Faure–Berlese fluid (50 g of gum arabic and 45 g of chloral hydrate dissolved in 80 g of distilled water and 60 cm3 of glycerol; Hille Ris Lambers 1950 ). All of the mentioned specimens were preserved in 95% ethanol and examined using calibrated oculars and an optical stereomicroscope (Olympus SZ 60 and Nikon Eclipse 80i). Using Corel Photo-Paint X7 and Corel Draw X7, drawings and outlines were created using a drawing tube (MNR-1) mounted on a stereomicroscope (Ampliwal). The larval instars’
body length (BL
, body width (BW
, and head capsule width (HW BL BW RL PW
body length (
, body width (
, and head capsule width (
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!