Tobramycin eye drops

New Zealand White rabbits weighing 2 to 2.5 kg were used for the study of the in vivo efficacy of εPL. The rabbits were anesthetized, and the corneal surface was deepithelialized with a sterile miniblade (Beaver; BD, MA, USA). Corneal infection was induced by applying 50 μl of 5 × 10⁶ CFU/ml S. aureus strain ATCC 29213 or P. aeruginosa strain ATCC 9027 to the scarified cornea. At 24 h postinfection, 50 μl of 0.3% (wt/vol) εPL (in PBS, pH 7.0) or PBS was applied topically to the infected eyes 4 times/day. Tobramycin eye drops (Alcon, Belgium), which contain 0.3% tobramycin, served as the positive control for P. aeruginosa keratitis, whereas gatifloxacin (Allergan, USA) eye drops were used as a positive control for S. aureus keratitis. Slit-lamp photographs and AS-OCT scans were taken before and after infection as well as during the course of treatment (39 (link), 40 (link)). The preinoculation and postinoculation corneal thicknesses (CTs) were measured perpendicular to the anterior corneal surfaces, and the average CT was reported (41 (link)).

DPP-Asp-hEGF showed a better biologically active property and was used for all further in vivo experiments consequently.
New Zealand white rabbits with a weight of 2.5 kg were chosen, and all experiments were conducted in accordance with the ARVO statement for the use of animals in ophthalmic research. Rabbits were anesthetized using 5 mg/kg zoletil (Virbac, Carlos, France), and oxybuprocaine eye drops (Santen, Osaka, Japan) were additionally applied for topical anesthesia during surgery.
A bulbar conjunctival defect of 7.5 mm diameter in the upper temporal part of the eye at a distance of 2 mm from the limbus was performed using a trephine. Rabbits were divided into three groups, with 6 rabbits per group. Defects were covered with DPP-Asp-hEGF, AM, or the sham operation (sham group). All transplants were sutured with eight single stich sutures using Vicryl 9-0.
Postoperatively, Tobramycin eye drops (Alcon, Fort Worth, USA), Tobramycin ointment (Alcon), and 0.5% Levofloxacin eye drops (Santen) were given one time every day for one week.

Mice were anesthetized using 1% pentobarbital sodium, tropicamide phenylephrine (Santen, Osaka, Japan), and procaine hydrochloride (Alcon, Fort Worth, TX, USA) eye drops were administrated here before the operation. Filter paper discs were prepared using a 2-mm-diameter trephine and sterilized for the next step. 1 µL of a solution of sodium hydroxide at a concentration of 1 mol L⁻¹ was dropped onto a filter paper disc. The excess liquid on the mice's ocular surface (or eyelids) was removed before the filter paper discs (with sodium hydroxide solution) were placed on the central cornea for 15 s. The ocular surface was then rinsed with 1 mL PBS 5 times. Mice were kept in a warm blanket until they woke up, and tobramycin eye drops (Alcon) and carbomer eye gel (Bausch & Lomb, Tampa, FL, USA) were applied once. The different concentrations of MOF eye drops (1, 0.5, 0.25 mg mL⁻¹) were topically administered (5 μL each eye) on the murine corneal alkali burn model three times daily (09:00 am, 3:00 pm, and 9:00 pm). The model group received the same volume of PBS as a substitute.