Anti erk2 d 2 sc 1647

The cell lines THP-1, SupT1, TZM-bl and H9/HTLV- IIIB along with anti-human APOBEC3G and anti-human APOBEC3F antibody were obtained from the NIH AIDS Research and Reference Reagent Program. THP-1 and Sup T1 were cultured in regular RPMI medium with 10% fetal calf serum (FBS). TZM-bl was grown in DMEM supplemented with 10% fetal calf serum. Immortalized human keratinocytes OKF6/TERT-2, provided by Dr. James G. Rheinwald (Harvard Medical School, MA) [41] (link), were cultured in Keratinocyte-SFM (Invitrogen) supplemented with 0.2 ng/ml recombinant epidermal growth factor (rEGF; Invitrogen), 25 µg/ml bovine pituitary extract (BPE) and 0.4 mM CaCl2 (final). Immortalized human vaginal epithelial cells VK2/E6E7, purchased from ATCC and cultured as described [42] (link). Lipopolysaccharide (LPS-EK Ultrapure) and lipoteichoic acid (LTA-SA) were purchased from Invivogen. MEK inhibitor U0126 was purchased from Calbiochem. Anti p-ERK (E-4) (sc-7383) and anti-ERK2 (D-2) (sc-1647) were purchased from Santa Cruz, USA.

Mouse monoclonal anti‐SHP2 (B‐1, sc‐7384), anti‐PDGFRβ‐pY716 (F‐10, sc‐365464), and anti‐ERK2 (D‐2, sc‐1647) antibodies, as well as rabbit polyclonal anti‐PDGFRβ (958, sc‐432) and anti‐PDGFRβpY579 (sc‐135671) antibodies, were purchased from Santa Cruz Biotechnology. Mouse monoclonal anti‐MEK1 (61B12, #2352), rabbit monoclonal anti‐PDGFRβpY857 (C43E9, #3170) and anti‐PDGFRβpY1009 (42F9, #3124) antibodies, as well as rabbit polyclonal anti‐pMEK S217/221 (#9121), anti‐pERK1/2 T202/Y204 (#9101), and anti‐EGFR (#2232) antibodies, were purchased from Cell Signaling. Mouse monoclonal anti‐phosphotyrosine antibody cocktail (4G10 platinum, 05‐1050) was purchased from Millipore. All antibodies were used at the concentrations recommended by their manufacturers.