Cordycepin

The samples were thawed and centrifuged at 15,000× g at 4°C for 10 min. The supernatant was mixed with 2% methanol at a 1:1 ratio and filtered through a 0.45 μm filter (Cat No. HAWP04700; Millipore, Billerica, MA) before analysis. The cordycepin concentration in the supernatant was measured using high performance liquid chromatography with a UV detector at 260 nm (Shimadzu, Tokyo, Japan). The TSK‐gel ODS‐80Ts (Tosoh Corp., Tokyo, Japan) was used at 40°C with 0.1% phosphoric acid: methanol at a 98:2 ratio (v/v) as the mobile phase (Masuda, Das, Hatashita, Fujihara, & Sakurai, 2014). cordycepin (Wako Pure Chem. Ind. Ltd., Osaka, Japan) was used as the reference standard.

Samples were thawed and centrifuged at 15,000×g at 4°C for 10 min. The supernatant was mixed with 2% methanol at a 1:1 ratio and filtered through a 0.45 μm filter (Cat No. HAWP04700, Millipore, Billerica, MA, USA) before analysis. The cordycepin concentration in the supernatant was measured by high-performance liquid chromatography (HPLC) with an UV detector at 260 nm (Shimadzu, Tokyo, Japan). The TSK-gel ODS-80Ts (Tosoh Corp., Tokyo, Japan) was used at 40°C with 0.1% phosphoric acid: methanol at a 98:2 ratio (v/v) was used as the mobile phase [8 ]. cordycepin (Wako Pure Chem. Ind. Ltd., Osaka, Japan) was used as the reference standard.