Ph indicator paper

Conductivity was measured using a Thermo Scientific Orion Versa Star Pro Advanced Electrochemistry Meter. Pre-tests were performed to observe if the results of the conductivity were directly proportional in acidic or alkaline solutions from 1 to 10% concentrated solutions. Since this condition has been verified, only 1% and 2% concentrations were used for the conductivity tests. Mats with an area of 9 cm² were immersed in alkali or acid solutions of NaOH and HCl. For the 1% (v/v) solutions, a single mat was immersed, whilst for the 2% (v/v) solutions two mats were required (18 cm²). Conductivity was measured over a period of 20 h with reading intervals of 5 s in the first 20 min, and in intervals of 20 min until 20 h. pH measurements were performed using a Thermo Scientific Orion Versa Star Pro Advanced Electrochemistry Meter and pH-indicator paper (Merck).

All reagents were purchased from Sigma-Aldrich unless otherwise stated and were used without further purification. The chelating agents p-SCN-Bn-DFO and p-SCN-Bn-DTPA were purchased from Macrocyclics Inc. (Dallas, TX). Water was deionised using a Barnstead NANOpure purification system (Thermo Scientific) and had a resistance of >18.2 MΩ cm⁻¹ at 25 °C. Protein concentration measurements were made on a ND-1000 spectrophotometer (NanoDrop Technologies, Inc.). Instant thin-layer chromatography (iTLC) was performed on glass microfiber chromatography paper (Agilent Technologies) and strips were analysed with a Bioscan AR-2000 radio-TLC scanner (Eckert & Ziegler). pH was determined using pH indicator paper (Merck Millipore). Radioactivity measurements were made using a CRC-25R dose calibrator (Capintec, Inc.).

All reagents were purchased from Fisher Scientific unless otherwise stated and used without further purification. Water was deionized using a Select Fusion ultrapure water deionisation unit (Suez) and had a resistance of >18.2 MΩ cm⁻¹ at 25 °C. Protein concentration measurements were obtained using a NanoDrop One^C Microvolume UV-vis Spectrophotometer (NanoDrop Technologies, Inc.). MALDI-TOF mass spectrometry measurements were taken on a Bruker Microflex LRF. Radioactivity measurements were obtained using a CRC-25 Dose Calibrator (Capintec, Inc.) or a Wizard 2480 Gamma Counter (PerkinElmer). Radioimmunoconjugate synthesis and serum stability studies were monitored by instant thin-layer chromatography using glass microfiber chromatography paper (iTLC-SA, Agilent). Radio-iTLC strips were measured by autoradiography (Amersham Typhoon Bioimager, GE) and analysed using ImageQuant software (GE Healthcare). pH measurements were determined using pH indicator paper (Merck Millipore) or a pH Spear electrode (Eutech Instruments).