Hgapdh forward

Total RNAs were isolated from CB-PIC treated HepG2 and Huh7 cells by using RNeasy mini kit (Qiagen). Quantitative reverse transcription PCR (RT-qPCR) was carried out under the LightCycler TM instrument (Roche Applied Sciences, Indianapolis, IN, USA) by using the following primers, IL-6- forward: 5′-CCACCGGGAACGAAAGAGAA-3′; reverse-: 5′-GAGAAGGCAACTGGACCGAA–3′ (Bioneer, Daejeon, Korea); hGAPDH-forward: 50-CCA CTC CTC CAC CTT TGA CA-30; reverse-: 50-ACC CTG TTG CTG TAG CCA-3 0 (Bioneer, Daejeon, Korea).

Total RNA was isolated from LncRNA RAB5IF depleted or intact HepG2 and Hep3B cells by using RNeasy mini kit (Qiagen) and reverse-transcribed using M-MLV reverse transcriptase (Promega, Madison, WI). Quantitative reverse transcription PCR (RT-qPCR) was conducted with the LightCycler TM instrument (Roche Applied Sciences, Indianapolis, IN, USA) using the following primers, LncRNA RAB5IF- forward: 5′-AGTCTCCGTCTGGAGTAAGGTG−3′; reverse- 5′-CCTGCTATTCCCAAGAACCCTC–3′ (Bioneer, Daejeon, Korea), LGR5 forward: 5′- CGTTGCAACACTGTCATGGC-3′; reverse- 5- AGGTCAGGTGAAGCGCTCG−3′ (Bioneer, Daejeon, Korea), hGAPDH-forward 5′-CCA CTC CTC CAC CTT TGA CA-3′; reverse-5′-ACC CTG TTG CTG TAG CCA −3′ (Bioneer, Daejeon, Korea).

As shown in Matsuno et al.’s paper, the total RNA from the LA-treated MCF-7 cells was isolated by QIAzol (Invitrogen, Carlsbad, CA, USA) and subjected to regular processes of RT-qPCR using the following primers, IL-6-forward: 5′-CCACCGGGAACGAAAGAGAA-3′; reverse-5′-GAGAAGGCAACTGGACCGAA -3′ (Bioneer, Daejeon, Korea), TNF- α- forward: 5′- GCCGCATCGCCGTCTCCTAC-3′; reverse- 5′-CCTCAGCCCCTCTGGGGTC -3′ (Bioneer, Daejeon, Korea), hGAPDH-forward5′-CCA CTC CTC CAC CTT TGA CA-3′;reverse-5′-ACC CTG TTG CTG TAG CCA -3′ (Bioneer, Daejeon, Korea). The mRNA level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used to normalize the expression of the genes of interest. Each sample was tested in triplicate, and the relative gene expression data were analyzed by means of the 2^−ΔCT method.