IFI6 was obtained from SAB Co. (Maryland, USA). GO and SA were prepared by RuiXi Materials Tech Co. (Xian, China). Dopamine was obtained from Sigma-Aldrich Co. (Shanghai, China). Anti-IFI6 and anti-phospho-HSF1 were purchased from Bioss Co. (Beijing, China). Anti-SSBP1 was purchased from FineTest Co (Wuhan, China).
To synthesize PDA@GO: 30 mg GO, we added 10 mm Tris buffer (pH 8.5) and placed it in a water bath ultrasound for 2 h. We then added 30 mg DA, stirred at room temperature for 48 h, centrifuged at 8000 rpm for 10 min, washed three times with water and ethanol, and dried at 40 ℃ for 12 h.
We synthesized IFI6- PDA@GO /SA: Dissolve SA in a 100-ml beaker, then added PDA@GO, 50 µl peroxide solution, and horseradish peroxidase. Finally, we added 3 mg IFI6 protein powder into the beaker and stirred for 1 h until the mixture was a hydrogel at room temperature (25 ℃).
To synthesize PDA@GO: 30 mg GO, we added 10 mm Tris buffer (pH 8.5) and placed it in a water bath ultrasound for 2 h. We then added 30 mg DA, stirred at room temperature for 48 h, centrifuged at 8000 rpm for 10 min, washed three times with water and ethanol, and dried at 40 ℃ for 12 h.
We synthesized IFI6- PDA@GO /SA: Dissolve SA in a 100-ml beaker, then added PDA@GO, 50 µl peroxide solution, and horseradish peroxidase. Finally, we added 3 mg IFI6 protein powder into the beaker and stirred for 1 h until the mixture was a hydrogel at room temperature (25 ℃).