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Api4000 qtrap mass spectrometer

Manufactured by Shimadzu
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Sourced in France

The API4000 Qtrap mass spectrometer is a high-performance instrument designed for advanced analytical applications. It combines the capabilities of a triple quadrupole (Qtrap) mass spectrometer with enhanced sensitivity and resolution. The core function of the API4000 Qtrap is to provide accurate mass identification and quantification of a wide range of analytes, enabling researchers and analysts to perform sensitive and reliable analyses.

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Lab products found in correlation

Hplc system, by Shimadzu (1 mentions) Hplc autosampler, by Shimadzu (1 mentions)

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API4000 mass spectrometer Spectrometers

2 protocols using api4000 qtrap mass spectrometer

1

Mass Spectrometry Quantification of Drugs in Serum and Brain

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One volume of brain or serum samples was added to three volumes of human serum EDTA K3, and the mixture was homogenized under magnetic stirring for 15 min for analysis using a Sciex API4000 Qtrap mass spectrometer coupled to a Shimadzu HPLC system (Shimadzu Corporation, Marne-la-Vallée, France). Liquid chromatography–MS procedures yielded detection ranges for DPZ from 0.1 to 100 ng/mL in serum and 0.1 to 100 ng/g in brain, and for MEF from 0.5 to 500 ng/mL in serum and 0.5 to 500 ng/g in brain.
Droguerre M., Duchêne A., Picoli C., Portal B., Lejards C., Guiard B.P., Meunier J., Villard V., Déglon N., Hamon M., Mouthon F, & Charvériat M. (2020). Efficacy of THN201, a Combination of Donepezil and Mefloquine, to Reverse Neurocognitive Deficits in Alzheimer’s Disease. Frontiers in Neuroscience, 14, 563.
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2

Quantification of cGAMP in Macrophages and BM-DCs

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cGAMP was quantified via LC/MS/MS at Climax Laboratories (San Jose, CA). Briefly, one million macrophages or BM-DCs were stimulated with DNA for one hour and then resuspended in PBS. cGAMP was extracted with 100% acetonitrile and analyzed by an LC/MS/MS system, Sciex API-4000Qtrap Mass Spectrometer and a Shimadzu HPLC/Autosampler with an ACE C18 column (2.1×100mm, 5μm). Positive Electronic Spray Ionization (ESI) and multiple reactions monitor (MRM) were used. The MRM transition of the test compound was 675/136 (m/z). The HPLC mobile phase A and B was 0.5% Formic acid in 5 mM NH4Ac solution and Acetonitrile/water (9/1) with 0.5% Formic acid. A related compound, Rp,Rp-c-diAMPSS, was used as an internal standard. The limit of quantification was 1.0 ng/mL and the dynamic range was 1.0–500 ng/mL.
Corrales L., Woo S.R., Williams J., McWhirter S.M., Dubensky T.W, & Gajewski T.F. (2016). Antagonism of the STING pathway via activation of the AIM2 inflammasome by intracellular DNA. Journal of immunology (Baltimore, Md. : 1950), 196(7), 3191-3198.
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