Chat cre 129s6 chattm2 cre lowl j

All AAV was produced by the University of North Carolina Chapel Hill Vector Core. Adult female C57BL/6 mice (Charles River Laboratories, Inc.) or Chat-Cre mice (Chat-cre;129S6-Chattm2(cre)Lowl/J, the Jackson Laboratory), 8–12 weeks at the time of surgery, were used for all experiments. AAV-Syn-SomArchon (5.9e12 genome copies (GC)/ml) or AAV-syn-SomArchon-P2A-CoChR-Kv2.1 (2.19e13 GC/ml) was injected into the motor cortex (AP: +1.5, ML: +/−1.5, DV: −0.3, 0.5uL virus), visual cortex (AP: −3.6, ML: +/−2.5, DV: −0.3, 0.5uL virus), hippocampus (AP:−2.0, ML:+1.4, DV:−1.6, 1uL virus) or striatum (AP:+0.8, ML:−1.8, DV:−2.1, 1uL virus). Viral injection occurred at 50–100nL/min (ten minutes total) using a 10uL syringe (NANOFIL, World Precision Instruments LLC) fitted with a 33 gauge needle (World Precision Instruments LLC, NF33BL) and controlled by a microinfusion pump (World Precision Instruments LLC, UltraMicroPump3–4). The syringe was left in place for an additional 10 minutes following injection to facilitate viral spread. About one week following the viral injection, mice underwent a second surgery to implant the cranial window for in vivo imaging.