Log In Sign up
The largest database of trusted experimental protocols

Cv5030 coverslipper

Manufactured by Leica
Visit Supplier
Sourced in Germany

The Leica CV5030 coverslipper is a laboratory instrument designed to automate the coverslipping process. It applies a protective glass coverslip over prepared microscope slides, ensuring consistent and efficient slide preparation.

Automatically generated - may contain errors

Lab products found in correlation

Superfrost plus slide, by Thermo Fisher Scientific (2 mentions) Ventana discovery ultra instrument, by Roche (2 mentions) Aperio at turbo scanner, by Leica (2 mentions) Discovery xt autostainer, by Roche (2 mentions) Autostainer xl, by Leica (2 mentions) St5010 autostainer, by Leica (1 mentions) Cryostar nx50, by Leica (1 mentions) Background sniper, by Biocare Medical (1 mentions) Vector immpact dab, by Vector Laboratories (1 mentions) St5020, by Leica (1 mentions) Af 307 na, by R&D Systems (1 mentions) M o m blocking reagent, by Vector Laboratories (1 mentions) Mom diluent, by Vector Laboratories (1 mentions)

Spelling variants of this product

CV5030 (31 citations) Leica CV5030 Glass Coverslipper (6 citations) CV5030 Coverslipper Workstation (3 citations) ST5020 &CV5030 Coverslipper (2 citations) Multistainer Stainer/Coverslipper Combo (ST5020-CV5030) (2 citations) Leica CV5030 automated coverslipper (2 citations) ST5020‐CV5030 Multistainer‐Coverslipper (1 citations)

8 protocols using cv5030 coverslipper

1

Hematoxylin & Eosin Histological Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Hematoxylin & eosin (H&E) histological staining was completed at the Monash Histology Platform at Monash University using a Leica ST5010 Autostainer and CV5030 Coverslipper. Imaging was completed using an Olympus Provis AX70 Widefield brightfield microscope at the Monash Micro Imaging (MMI) facility at Monash University.
Lindsey B.W., Aitken G.E., Tang J.K., Khabooshan M., Douek A.M., Vandestadt C, & Kaslin J. (2019). Midbrain tectal stem cells display diverse regenerative capacities in zebrafish. Scientific Reports, 9, 4420.
+ Open protocol
+ Expand
2

Cryosectioning and Histological Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Frozen sections of haired skin were stored in a −80 °C ultralow freezer prior to sectioning on a cryotome. The sections of skin were cut 10 μm thick using a Thermo Scientific CryoStar NX50, routinely stained with hematoxylin and eosin on a Leica ST5020 H&E stainer, and coverslipped with a Leica CV5030 coverslipper. A veterinary anatomic pathologist who is board certified by the American College of Veterinary Pathologists performed all histological evaluations.
Derakhshandeh H., Aghabaglou F., McCarthy A., Mostafavi A., Wiseman C., Bonick Z., Ghanavati I., Harris S., Kreikemeier-Bower C., Basri S.M., Rosenbohm J., Yang R., Mostafalu P., Orgill D, & Tamayol A. (2020). A Wirelessly Controlled Smart Bandage with 3D-Printed Miniaturized Needle Arrays. Advanced functional materials, 30(13), 1905544.
+ Open protocol
+ Expand
3

Immunohistochemistry of H3.3 Mutations

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry (IHC) was performed at Histology Platform (RI-MUHC) and the Segal Cancer Centre Research Pathology Facility (Jewish General Hospital). PDOX and mouse brain tissue samples were cut at 4-6 μm, placed on SuperFrost/Plus slides (Fisher) and dried overnight at 37°C, before IHC processing. After de-paraffinization and epitope retrieval, sections were incubated with primary antibodies: Gsx2 (ABN162, Millipore Sigma RRID:AB_11203296) in 1:100; H3.3 G34R (RM240 , RevMAb Biosciences, RRID:AB_2716433) in 1:50; and H3.3 G34V (RM307, RevMAb Biosciences RRID:AB_2716435) in 1:40. Slides were then loaded onto the Discovery XT Autostainer or Ventana Discovery Ultra Instrument (Ventana Medical Systems). Slides were counterstained with hematoxylin, blued with Bluing Reagent, washed, dehydrated through graded alcohols, cleared in xylene, and mounted with mounting medium (Eukitt, Fluka Analytical) or Leica CV 5030 coverslipper. Sections were analyzed by conventional light microscopy or scanned using the Aperio AT Turbo Scanner (Leica Biosystems).
Chen C.C., Deshmukh S., Jessa S., Hadjadj D., Lisi V., Andrade A.F., Faury D., Jawhar W., Dali R., Suzuki H., Pathania M., A D., Dubois F., Woodward E., Hébert S., Coutelier M., Karamchandani J., Albrecht S., Brandner S., De Jay N., Gayden T., Bajic A., Harutyunyan A.S., Marchione D.M., Mikael L.G., Juretic N., Zeinieh M., Russo C., Maestro N., Bassenden A.V., Hauser P., Virga J., Bognar L., Klekner A., Zapotocky M., Vicha A., Krskova L., Vanova K., Zamecnik J., Sumerauer D., Ekert P.G., Ziegler D.S., Ellezam B., Filbin M.G., Blanchette M., Hansford J.R., Khuong-Quang D.A., Berghuis A.M., Weil A.G., Garcia B.A., Garzia L., Mack S.C., Beroukhim R., Ligon K.L., Taylor M.D., Bandopadhayay P., Kramm C., Pfister S.M., Korshunov A., Sturm D., Jones D.T., Salomoni P., Kleinman C.L, & Jabado N. (2020). Histone H3.3G34-mutant interneuron progenitors co-opt PDGFRA for gliomagenesis. Cell, 183(6), 1617-1633.e22.
+ Open protocol
+ Expand
4

Immunohistochemical Staining of LV9 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
7μm frozen sections were dried overnight then fixed in a 3:1 mix of anhydrous acetone/ethanol for 5 minutes. Endogenous peroxidase was blocked using 0.5% (v/v) hydrogen peroxide in methanol for 10 minutes and non-specific binding of the primary antibody minimized by incubating sections in Biocare Medical Background Sniper plus 2.0% (w/v) BSA for 10 minutes. The primary antibody, hamster anti-mouse LV9 diluted 1:1000 in Biocare Medical Da Vinci Green antibody diluent, was applied for 60 minutes then detected by applying Jackson Immunoresearch goat anti-hamster secondary, diluted 1:300 in TBS, for 30 minutes followed by a 30 minute application of Vector ImmPRESS Goat HRP polymer. LV9 signal was visualized using Vector ImmPACT DAB. Sections were counterstained with Mayer’s Haematoxylin in a Leica Autostainer XL and mounted using a Leica CV5030 coverslipper.
Montes de Oca M., de Labastida Rivera F., Winterford C., Frame T.C., Ng S.S., Amante F.H., Edwards C.L., Bukali L., Wang Y., Uzonna J.E., Kuns R.D., Zhang P., Kabat A., Klein Geltink R.I., Pearce E.J., Hill G.R, & Engwerda C.R. (2020). IL-27 signalling regulates glycolysis in Th1 cells to limit immunopathology during infection. PLoS Pathogens, 16(10), e1008994.
+ Open protocol
+ Expand
5

Tissue Processing for Brightfield and Darkfield Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
10% neutral buffered formalin-fixed tissues were placed in labeled tissue processing cassettes and processed overnight into paraffin using an automated tissue processor (Leica ASP6025S). Processed tissues were then embedded into paraffin blocks and sectioned at a thickness of 4 μm. Sections from three levels spaced 50 μm apart were collected and mounted onto microscope slides (two slides per level; one for Hematoxylin & Eosin staining and another for coverslipping only).
After air drying overnight, the slides were loaded into a robotic stainer and coverslipper (Autostainer XL and CV5030 coverslipper; Leica), baked at 65 °C for 15 min and then de-waxed in xylene. Half of the slides were then coverslipped with a resinous mounting medium for darkfield imaging and the other half were stained with Hematoxylin & Eosin (H&E) according to standard procedures for brightfield images.
Bromma K., Dos Santos N., Barta I., Alexander A., Beckham W., Krishnan S, & Chithrani D.B. (2022). Enhancing nanoparticle accumulation in two dimensional, three dimensional, and xenograft mouse cancer cell models in the presence of docetaxel. Scientific Reports, 12, 13508.
+ Open protocol
+ Expand
6

Paraffin Embedding and Histological Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
For paraffin embedding, tissue was fixed overnight in 2% PFA, placed in 70% EtOH, and processed using the Leica ASP6025 autoprocessor. The program was set to 12 h standard dehydration and paraffin saturation protocol. After processing, tissue samples were embedded into formalin-fixed paraffin blocks upside down by cutting their surface and orienting the blocks transversally. Standard hematoxylin and eosin staining, dehydration, and placing the samples on coverslips were accomplished using the Leica ST5020 automated staining instrument in combination with the Leica CV5030 cover-slipper.
Vacurova E., Trnovska J., Svoboda P., Skop V., Novosadova V., Reguera D.P., Petrezselyová S., Piavaux B., Endaya B., Spoutil F., Zudova D., Stursa J., Melcova M., Bielcikova Z., Werner L., Prochazka J., Sedlacek R., Huttl M., Hubackova S.S., Haluzik M, & Neuzil J. (2022). Mitochondrially targeted tamoxifen alleviates markers of obesity and type 2 diabetes mellitus in mice. Nature Communications, 13, 1866.
+ Open protocol
+ Expand
7

Immunohistochemistry Assay for G34R/V Gliomas

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry (IHC) was performed at Histology Platform (RI-MUHC) and the Segal Cancer Centre Research Pathology Facility (Jewish General Hospital). G34R/V high-grade glioma samples were cut at 4-6 μm, placed on SuperFrost/Plus slides (Fisher) and dried overnight at 37°C, before IHC processing. After de-paraffinization and epitope retrieval, sections were incubated with primary antibodies: phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204, 4376 Cell Signaling, RRID:AB_331772) in 1:100; and PDGFRA (AF-307-NA, R&D Systems, 1:100, RRID:AB_354459). Slides were then loaded onto the Discovery XT Autostainer or Ventana Discovery Ultra Instrument (Ventana Medical Systems). Slides were counterstained with hematoxylin, blued with Bluing Reagent, washed, dehydrated through graded alcohols, cleared in xylene, and mounted with mounting medium (Eukitt, Fluka Analytical) or Leica CV 5030 coverslipper. Sections were analyzed by conventional light microscopy or scanned using the Aperio AT Turbo Scanner (Leica Biosystems).
Chen C.C., Deshmukh S., Jessa S., Hadjadj D., Lisi V., Andrade A.F., Faury D., Jawhar W., Dali R., Suzuki H., Pathania M., A D., Dubois F., Woodward E., Hébert S., Coutelier M., Karamchandani J., Albrecht S., Brandner S., De Jay N., Gayden T., Bajic A., Harutyunyan A.S., Marchione D.M., Mikael L.G., Juretic N., Zeinieh M., Russo C., Maestro N., Bassenden A.V., Hauser P., Virga J., Bognar L., Klekner A., Zapotocky M., Vicha A., Krskova L., Vanova K., Zamecnik J., Sumerauer D., Ekert P.G., Ziegler D.S., Ellezam B., Filbin M.G., Blanchette M., Hansford J.R., Khuong-Quang D.A., Berghuis A.M., Weil A.G., Garcia B.A., Garzia L., Mack S.C., Beroukhim R., Ligon K.L., Taylor M.D., Bandopadhayay P., Kramm C., Pfister S.M., Korshunov A., Sturm D., Jones D.T., Salomoni P., Kleinman C.L, & Jabado N. (2020). Histone H3.3G34-mutant interneuron progenitors co-opt PDGFRA for gliomagenesis. Cell, 183(6), 1617-1633.e22.
+ Open protocol
+ Expand
8

Immunohistochemical Labeling of Cryosections

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cryosections were air-dried for 45 min, washed in PBS for 10 min, treated with freshly prepared 1 mg/ml sodium borohydride/PBS solution for 4 min, washed in PBS again and subsequently blocked with M.O.M. blocking reagent (Vector Laboratories, Burlingame, CA, USA) in 0.1 %TritonX-100/PBS for 60 min in a humid chamber. After that, sections were washed 3×5 min in PBS and incubated with primary antibodies (see labeling below) in M.O.M. diluent (Vector Laboratories) over night at 4°C in a humid chamber. Binding of the primary antibodies was visualized using highly cross-absorbed labeled secondary antibodies (see labeling below) for 60 min in a damp and light-protected chamber at RT. After further washing in PBS, slides were incubated with 4, 6-Diamidin-2-phenylindol (DAPI, PanReac AppliChem GmbH, Darmstadt, Germany) for 15 min to stain cell nuclei and afterwards covered with Mowiol and cover slips using a Leica CV5030 coverslipper.
Hinteregger B., Loeffler T., Flunkert S., Neddens J., Bayer T.A., Madl T, & Hutter-Paier B. (2021). Metabolic, Phenotypic, and Neuropathological Characterization of the Tg4-42 Mouse Model for Alzheimer’s Disease. Journal of Alzheimer's Disease, 80(3), 1151-1168.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!